Detection of HIV RNA by in situ hybridization in peripheral blood mononuclear cells of seronegative children born to HIV-infected mothers

1990 ◽  
Vol 149 (5) ◽  
pp. 330-332 ◽  
Author(s):  
V. Wahn ◽  
S. Sauer ◽  
S. Vollbach ◽  
A. Immelmann ◽  
B. Neumann ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Hiv Rna ◽  
Blood Mononuclear Cells

scholarly journals Detection of hepatitis C virus RNA in peripheral blood mononuclear cells of infected patients by in situ hybridization

Blood ◽  
1994 ◽  
Vol 83 (1) ◽  
pp. 269-273 ◽  
Author(s):  
J Moldvay ◽  
P Deny ◽  
S Pol ◽  
C Brechot ◽  
E Lamas
Keyword(s):  
In Situ Hybridization ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Hcv Rna ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

We used in situ hybridization to detect hepatitis C virus (HCV) infection of peripheral blood mononuclear cells (PBMNC) from 11 patients with chronic active hepatitis. Using 35S-labeled HCV-RNA probe, HCV-RNA-positive and -negative strands were observed in unstimulated PBMNC from three patients, all of whom were receiving immunosuppressive drugs after orthotopic liver transplantation (OLT). HCV-RNA sequences were also identified in PBMNC from three patients who were not undergoing immunosuppression, after stimulation with either phytohemagglutinin (PHA) or pokeweed mitogen (PWM). In contrast, HCV- RNA was not found in the remaining five patients, who had not undergone OLT and whose cells were not stimulated with mitogens. These results show that mononuclear cells can be infected by HCV and that mitogenic stimulation of infected cells increases HCV-RNA replication.

scholarly journals Detection of hepatitis C virus RNA in peripheral blood mononuclear cells of infected patients by in situ hybridization

Blood ◽  
1994 ◽  
Vol 83 (1) ◽  
pp. 269-273 ◽  
Author(s):  
J Moldvay ◽  
P Deny ◽  
S Pol ◽  
C Brechot ◽  
E Lamas
Keyword(s):  
In Situ Hybridization ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Hcv Rna ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Abstract We used in situ hybridization to detect hepatitis C virus (HCV) infection of peripheral blood mononuclear cells (PBMNC) from 11 patients with chronic active hepatitis. Using 35S-labeled HCV-RNA probe, HCV-RNA-positive and -negative strands were observed in unstimulated PBMNC from three patients, all of whom were receiving immunosuppressive drugs after orthotopic liver transplantation (OLT). HCV-RNA sequences were also identified in PBMNC from three patients who were not undergoing immunosuppression, after stimulation with either phytohemagglutinin (PHA) or pokeweed mitogen (PWM). In contrast, HCV- RNA was not found in the remaining five patients, who had not undergone OLT and whose cells were not stimulated with mitogens. These results show that mononuclear cells can be infected by HCV and that mitogenic stimulation of infected cells increases HCV-RNA replication.

Arylamine N-acetyltransferase 1 in situ N-acetylation on CD3+ peripheral blood mononuclear cells correlate with NATb mRNA and NAT1 haplotype

2017 ◽  
Vol 92 (2) ◽  
pp. 661-668 ◽  
Author(s):  
Raúl A. Salazar-González ◽  
Eneida Turiján-Espinoza ◽  
David W. Hein ◽  
Perla C. Niño-Moreno ◽  
Silvia Romano-Moreno ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells
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