scholarly journals Insertion Sequence 1515 in the ply Gene of a Type 1 Clinical Isolate of Streptococcus pneumoniae Abolishes Pneumolysin Expression

2007 ◽  
Vol 45 (7) ◽  
pp. 2296-2297 ◽  
Author(s):  
F. Garnier ◽  
R. P. Janapatla ◽  
E. Charpentier ◽  
G. Masson ◽  
C. Grelaud ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Clinical Isolate ◽  
Insertion Sequence

scholarly journals Characterization of IS1515, a Functional Insertion Sequence in Streptococcus pneumoniae

1998 ◽  
Vol 180 (6) ◽  
pp. 1381-1388 ◽  
Author(s):  
Rosario Muñoz ◽  
Rubens López ◽  
Ernesto García
Keyword(s):  
Streptococcus Pneumoniae ◽  
Insertion Sequence ◽  
Frameshift Mutation ◽  
Lactobacillus Helveticus ◽  
Inverted Repeats ◽  
Insertion Sequences ◽  
Amino Acid Residues ◽  
Streptomyces Albus

ABSTRACT We describe the characterization of a new insertion sequence, IS1515, identified in the genome of Streptococcus pneumoniae I41R, an unencapsulated mutant isolated many years ago (R. Austrian, H. P. Bernheimer, E. E. B. Smith, and G. T. Mills, J. Exp. Med. 110:585–602, 1959). A copy of this element located in the cap1E I41R gene was sequenced. The 871-bp-long IS1515 element possesses 12-bp perfect inverted repeats and generates a 3-bp target duplication upon insertion. The IS encodes a protein of 271 amino acid residues similar to the putative transposases of other insertion sequences, namely IS1381 from S. pneumoniae, ISL2from Lactobacillus helveticus, IS702 from the cyanobacterium Calothrix sp. strain PCC 7601, and IS112 from Streptomyces albus G. IS1515 appears to be present in the genome of most type 1 pneumococci in a maximum of 13 copies, although it has also been found in the chromosome of pneumococcal isolates belonging to other serotypes. We have found that the unencapsulated phenotype of strain I41R is the result of both the presence of an IS1515 copy and a frameshift mutation in the cap1E I41Rgene. Precise excision of the IS was observed in the type 1 encapsulated transformants isolated in experiments designed to repair the frameshift. These results reveal that IS1515 behaves quite differently from other previously described pneumococcal insertion sequences. Several copies of IS1515 were also able to excise and move to another locations in the chromosome ofS. pneumoniae. To our knowledge, this is the first report of a functional IS in pneumococcus.

scholarly journals Acquisition of Broad-Spectrum Cephalosporin Resistance Leading to Colistin Resistance in Klebsiella pneumoniae

2016 ◽  
Vol 60 (5) ◽  
pp. 3199-3201 ◽  
Author(s):  
Aurélie Jayol ◽  
Patrice Nordmann ◽  
Marine Desroches ◽  
Jean-Winoc Decousser ◽  
Laurent Poirel
Keyword(s):  
Klebsiella Pneumoniae ◽  
Clinical Isolate ◽  
Broad Spectrum ◽  
Insertion Sequence ◽  
Selective Pressure ◽  
Acquired Resistance ◽  
Colistin Resistance ◽  
Content Type ◽  
Esbl Gene ◽  
Cephalosporin Resistance

ABSTRACTAn extended-spectrum β-lactamase (ESBL)-producing and colistin-resistantKlebsiella pneumoniaeclinical isolate was recovered from a patient who was treated with cefotaxime. This isolate harbored ablaCTX-M-15ESBL gene that was associated with an ISEcp1insertion sequence. Transposition of that tandem occurred within the chromosomalmgrBgene, leading to inactivation of themgrBgene and consequently to acquired resistance to colistin. We showed here a coselection of colistin resistance as a result of a broad-spectrum cephalosporin selective pressure.

scholarly journals Heterogeneous Macrolide Resistance and Gene Conversion in the Pneumococcus

2006 ◽  
Vol 50 (1) ◽  
pp. 359-361 ◽  
Author(s):  
Nicole Wolter ◽  
Anthony M. Smith ◽  
David J. Farrell ◽  
Keith P. Klugman
Keyword(s):  
Streptococcus Pneumoniae ◽  
Gene Conversion ◽  
Clinical Isolate ◽  
Resistant Strain ◽  
Macrolide Resistance ◽  
Fitness Cost ◽  
Rrna Genes ◽  
23S Rrna ◽  
Resistant Phenotype ◽  
23S Rrna Genes

ABSTRACT A macrolide-resistant clinical isolate of Streptococcus pneumoniae with 23S rRNA mutations showed a heterogeneous phenotype and genotype. The mutant 23S rRNA genes from this isolate transformed susceptible strain R6 to resistance. Culture of resistant strain R6 in the absence of antibiotic pressure showed gene conversion to occur between the four 23S rRNA alleles, resulting in reversion to susceptibility with the resistant phenotype showing a fitness cost. These data explain the disappearance on subculture of heterogeneous macrolide resistance in the pneumococcus.

scholarly journals Characterization of a Genetic Element Carrying the Macrolide Efflux Gene mef(A) in Streptococcus pneumoniae

2000 ◽  
Vol 44 (9) ◽  
pp. 2585-2587 ◽  
Author(s):  
Maria Santagati ◽  
Francesco Iannelli ◽  
Marco R. Oggioni ◽  
Stefania Stefani ◽  
Gianni Pozzi
Keyword(s):  
Streptococcus Pneumoniae ◽  
Resistance Gene ◽  
Clinical Isolate ◽  
Open Reading Frames ◽  
Genetic Element ◽  
Reading Frames

ABSTRACT The mef(A) gene from a clinical isolate ofStreptococcus pneumoniae exhibiting the M-type resistance to macrolides was found to be part of the 7,244-bp chromosomal element Tn1207.1, which contained 8 open reading frames.orf2 encodes a resolvase/invertase, and orf5 is a homolog of the macrolide-streptogramin B resistance genemsr(SA).

The IS1167 Insertion Sequence Is a Phylogenetically Informative Marker Among Isolates of Serotype 6B Streptococcus pneumoniae

1998 ◽  
Vol 47 (2) ◽  
pp. 222-229 ◽  
Author(s):  
D. Ashley Robinson ◽  
Susan K. Hollingshead ◽  
James M. Musser ◽  
Alan J. Parkinson ◽  
David E. Briles ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Insertion Sequence ◽  
Informative Marker

scholarly journals Mutation in 23S rRNA Responsible for Resistance to 16-Membered Macrolides and Streptogramins in Streptococcus pneumoniae

2001 ◽  
Vol 45 (1) ◽  
pp. 319-323 ◽  
Author(s):  
Florence Depardieu ◽  
Patrice Courvalin
Keyword(s):  
Escherichia Coli ◽  
Streptococcus Pneumoniae ◽  
Clinical Isolate ◽  
23S Rrna ◽  
Resistance Phenotype ◽  
Domain V

ABSTRACT Streptococcus pneumoniae clinical isolate BM4455 was resistant to 16-membered macrolides and to streptogramins. This unusual resistance phenotype was due to an A2062C (Escherichia coli numbering) mutation in domain V of the four copies of 23S rRNA.

Sign in / Sign up

Export Citation Format

Share Document