The IS1167 Insertion Sequence Is a Phylogenetically Informative Marker Among Isolates of Serotype 6B Streptococcus pneumoniae

1998 ◽  
Vol 47 (2) ◽  
pp. 222-229 ◽  
Author(s):  
D. Ashley Robinson ◽  
Susan K. Hollingshead ◽  
James M. Musser ◽  
Alan J. Parkinson ◽  
David E. Briles ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Insertion Sequence ◽  
Informative Marker

scholarly journals Insertion Sequence 1515 in the ply Gene of a Type 1 Clinical Isolate of Streptococcus pneumoniae Abolishes Pneumolysin Expression

2007 ◽  
Vol 45 (7) ◽  
pp. 2296-2297 ◽  
Author(s):  
F. Garnier ◽  
R. P. Janapatla ◽  
E. Charpentier ◽  
G. Masson ◽  
C. Grelaud ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Clinical Isolate ◽  
Insertion Sequence

scholarly journals Characterization of IS1515, a Functional Insertion Sequence in Streptococcus pneumoniae

1998 ◽  
Vol 180 (6) ◽  
pp. 1381-1388 ◽  
Author(s):  
Rosario Muñoz ◽  
Rubens López ◽  
Ernesto García
Keyword(s):  
Streptococcus Pneumoniae ◽  
Insertion Sequence ◽  
Frameshift Mutation ◽  
Lactobacillus Helveticus ◽  
Inverted Repeats ◽  
Insertion Sequences ◽  
Amino Acid Residues ◽  
Streptomyces Albus

ABSTRACT We describe the characterization of a new insertion sequence, IS1515, identified in the genome of Streptococcus pneumoniae I41R, an unencapsulated mutant isolated many years ago (R. Austrian, H. P. Bernheimer, E. E. B. Smith, and G. T. Mills, J. Exp. Med. 110:585–602, 1959). A copy of this element located in the cap1E I41R gene was sequenced. The 871-bp-long IS1515 element possesses 12-bp perfect inverted repeats and generates a 3-bp target duplication upon insertion. The IS encodes a protein of 271 amino acid residues similar to the putative transposases of other insertion sequences, namely IS1381 from S. pneumoniae, ISL2from Lactobacillus helveticus, IS702 from the cyanobacterium Calothrix sp. strain PCC 7601, and IS112 from Streptomyces albus G. IS1515 appears to be present in the genome of most type 1 pneumococci in a maximum of 13 copies, although it has also been found in the chromosome of pneumococcal isolates belonging to other serotypes. We have found that the unencapsulated phenotype of strain I41R is the result of both the presence of an IS1515 copy and a frameshift mutation in the cap1E I41Rgene. Precise excision of the IS was observed in the type 1 encapsulated transformants isolated in experiments designed to repair the frameshift. These results reveal that IS1515 behaves quite differently from other previously described pneumococcal insertion sequences. Several copies of IS1515 were also able to excise and move to another locations in the chromosome ofS. pneumoniae. To our knowledge, this is the first report of a functional IS in pneumococcus.

scholarly journals Interruption of the Streptococcus gordonii M5 sspA/sspB intergenic region by an insertion sequence related to IS1167 of Streptococcus pneumoniae

Microbiology ◽  
1997 ◽  
Vol 143 (6) ◽  
pp. 2047-2055 ◽  
Author(s):  
D. R. Demuth ◽  
Y. Duan ◽  
H. F. Jenkinson ◽  
R. McNab ◽  
S. Gil ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Intergenic Region ◽  
Insertion Sequence ◽  
Streptococcus Gordonii

scholarly journals erm(B)-Carrying Elements in Tetracycline-Resistant Pneumococci and Correspondence between Tn1545 and Tn6003

2008 ◽  
Vol 52 (4) ◽  
pp. 1285-1290 ◽  
Author(s):  
Ileana Cochetti ◽  
Emily Tili ◽  
Marina Mingoia ◽  
Pietro E. Varaldo ◽  
Maria Pia Montanari
Keyword(s):  
Streptococcus Pneumoniae ◽  
Resistance Gene ◽  
Insertion Sequence ◽  
Reference Strain ◽  
Clinical Isolates ◽  
Erythromycin Resistance ◽  
M Gene ◽  
Genetic Organization ◽  
Gene Combination ◽  
Related Element

ABSTRACT This study investigated the genetic organization of erm(B)-carrying transposons of Streptococcus pneumoniae and their distribution in tetracycline-resistant clinical isolates. By comparatively analyzing reference pneumococci carrying erm(B)/tet(M) transposon Tn1545, Tn6003, Tn6002, or Tn3872, we demonstrated a substantial correspondence between Tn1545 and Tn6003, which have the same resistance gene combination [tet(M) (tetracycline), erm(B) (erythromycin), and aphA-3 (kanamycin)]; share the macrolide-aminoglycoside-streptothricin element, containing a second erm(B); and only differ by a ca. 1.2-kb insertion (containing a putative IS1239 insertion sequence) detected in Tn1545 from S. pneumoniae reference strain BM4200. These results enabled elucidation of the structure of Tn1545, the first erm(B)-carrying transposon described in S. pneumoniae. A collection of 83 erythromycin- and tetracycline-resistant clinical pneumococci, representative of recent Italian isolates carrying erm(B) as the sole erythromycin resistance gene, was used to investigate the distribution of the different transposons. All 83 organisms were positive for tet(M) and bore an erm(B)/tet(M) transposon that could be characterized by using a specific set of primer pairs; Tn3872 was detected in 18 isolates, Tn6002 in 59 isolates, and Tn6003 in 6 (the sole kanamycin-resistant) isolates. The genetic organization of transposon Tn1545, with its specific insertion, was not detected in any of the isolates tested. The erm(B)-carrying elements of tetracycline-resistant pneumococci substantially corresponded to those [bearing a silent tet(M) gene] recently detected in tetracycline-susceptible pneumococci. Overall, in erm(B)-positive pneumococci, Tn6003 was the least common erm(B)-carrying Tn916-related element and Tn6002 the most common.

scholarly journals Isolation, characterization, and nucleotide sequence of IS1202, an insertion sequence of Streptococcus pneumoniae.

1994 ◽  
Vol 176 (14) ◽  
pp. 4437-4443 ◽  
Author(s):  
J K Morona ◽  
A Guidolin ◽  
R Morona ◽  
D Hansman ◽  
J C Paton
Keyword(s):  
Streptococcus Pneumoniae ◽  
Nucleotide Sequence ◽  
Insertion Sequence

Characterization of IS1167, a New Insertion Sequence in Streptococcus pneumoniae

Plasmid ◽  
1995 ◽  
Vol 33 (2) ◽  
pp. 127-138 ◽  
Author(s):  
Lixin Zhou ◽  
Francis M. Hui ◽  
Donald A. Morrison
Keyword(s):  
Streptococcus Pneumoniae ◽  
Insertion Sequence

Impacto de los serotipos 22F y 33F en la epidemiología y patogénesis de Streptococcus pneumoniae causantes de enfermedad neumocócica invasiva en España

Vacunas ◽  
2019 ◽  
Vol 20 ◽  
pp. 36
Author(s):  
J. Sempere ◽  
F. González-Camacho ◽  
M.D. Vicioso ◽  
I. del Río Menéndez ◽  
J. Yuste ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae
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