scholarly journals The SMC-Like Protein Complex SbcCD Enhances DNA Polymerase IV-Dependent Spontaneous Mutation in Escherichia coli

2010 ◽  
Vol 193 (3) ◽  
pp. 660-669 ◽  
Author(s):  
K. A. M. Storvik ◽  
P. L. Foster
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Protein Complex ◽  
Spontaneous Mutation ◽  
Dna Polymerase Iv

scholarly journals Human and mouse homologs of Escherichia coli DinB (DNA polymerase IV), members of the UmuC/DinB superfamily

1999 ◽  
Vol 96 (21) ◽  
pp. 11922-11927 ◽  
Author(s):  
V. L. Gerlach ◽  
L. Aravind ◽  
G. Gotway ◽  
R. A. Schultz ◽  
E. V. Koonin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Dna Polymerase Iv ◽  
Human And Mouse

scholarly journals A strategically located serine residue is critical for the mutator activity of DNA polymerase IV from Escherichia coli

2013 ◽  
Vol 41 (9) ◽  
pp. 5104-5114 ◽  
Author(s):  
Amit Sharma ◽  
Jithesh Kottur ◽  
Naveen Narayanan ◽  
Deepak T. Nair
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Serine Residue ◽  
Mutator Activity ◽  
Dna Polymerase Iv

scholarly journals Discrimination against the Fluorescent Cytosine Analog tC by Escherichia coli DNA Polymerase IV DinB

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Jason Walsh ◽  
Imenne Bouamaied ◽  
Tom Brown ◽  
Marcus Wilhelmsson ◽  
Penny J Beuning
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Dna Polymerase Iv

scholarly journals Escherichia coli DNA Polymerase IV Mutator Activity: Genetic Requirements and Mutational Specificity

2000 ◽  
Vol 182 (16) ◽  
pp. 4587-4595 ◽  
Author(s):  
Jérôme Wagner ◽  
Takehiko Nohmi
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Amino Acid Level ◽  
Nucleotide Substitutions ◽  
Pol Iv ◽  
Mutator Activity ◽  
Sequence Homologies ◽  
In The Wild ◽  
Dna Polymerase Iv ◽  
Dna Pol Iv

ABSTRACT The dinB gene of Escherichia coli is known to be involved in the untargeted mutagenesis of λ phage. Recently, we have demonstrated that this damage-inducible and SOS-controlled gene encodes a novel DNA polymerase, DNA Pol IV, which is able to dramatically increase the untargeted mutagenesis of F′ plasmid. At the amino acid level, DNA Pol IV shares sequence homologies with E. coli UmuC (DNA Pol V), Rev1p, and Rad30p (DNA polymerase η) ofSaccharomyces cerevisiae and human Rad30A (XPV) proteins, all of which are involved in translesion DNA synthesis. To better characterize the Pol IV-dependent untargeted mutagenesis, i.e., the DNA Pol IV mutator activity, we analyzed the genetic requirements of this activity and determined the forward mutation spectrum generated by this protein within the cII gene of λ phage. The results indicated that the DNA Pol IV mutator activity is independent ofpolA, polB, recA,umuDC, uvrA, and mutS functions. The analysis of more than 300 independent mutations obtained in the wild-type or mutS background revealed that the mutator activity clearly promotes single-nucleotide substitutions as well as one-base deletions in the ratio of about 1:2. The base changes were strikingly biased for substitutions toward G:C base pairs, and about 70% of them occurred in 5′-GX-3′ sequences, where X represents the base (T, A, or C) that is mutated to G. These results are discussed with respect to the recently described biochemical characteristics of DNA Pol IV.

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