scholarly journals Mutagenicity and Genotoxicity of (5′S)-8,5′-Cyclo-2′-deoxyadenosine in Escherichia coli and Replication of (5′S)-8,5′-Cyclopurine-2′-deoxynucleosides in Vitro by DNA Polymerase IV, Exo-Free Klenow Fragment, and Dpo4

2014 ◽  
Vol 27 (2) ◽  
pp. 200-210 ◽  
Author(s):  
Varsha Pednekar ◽  
Savithri Weerasooriya ◽  
Vijay P. Jasti ◽  
Ashis K. Basu
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Klenow Fragment ◽  
Dna Polymerase Iv

scholarly journals Involvement of Y-Family DNA Polymerases in Mutagenesis Caused by Oxidized Nucleotides in Escherichia coli

2006 ◽  
Vol 188 (13) ◽  
pp. 4992-4995 ◽  
Author(s):  
Masami Yamada ◽  
Tatsuo Nunoshiba ◽  
Masatomi Shimizu ◽  
Petr Gruz ◽  
Hiroyuki Kamiya ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Dna Polymerases ◽  
Dna Polymerase Iv ◽  
Y Family

ABSTRACT Escherichia coli DNA polymerase IV incorporated 2-hydroxy-dATP opposite template guanine or thymine and 8-hydroxy-dGTP exclusively opposite adenine in vitro. Mutator phenotypes in sod/fur strains were substantially diminished by deletion of dinB and/or umuDC. DNA polymerases IV and V may be involved in mutagenesis caused by incorporation of the oxidized deoxynucleoside triphosphates.

scholarly journals Minidumbbell structures formed by ATTCT pentanucleotide repeats in spinocerebellar ataxia type 10

2020 ◽  
Author(s):  
Pei Guo ◽  
Sik Lok Lam
Keyword(s):  
Spinocerebellar Ataxia ◽  
Dna Polymerase ◽  
Dna Sequences ◽  
Solution Structure ◽  
Large Scale ◽  
Genetic Disorder ◽  
Spinocerebellar Ataxia Type ◽  
Klenow Fragment ◽  
Repeat Expansions

Abstract Spinocerebellar ataxia type 10 (SCA10) is a progressive genetic disorder caused by ATTCT pentanucleotide repeat expansions in intron 9 of the ATXN10 gene. ATTCT repeats have been reported to form unwound secondary structures which are likely linked to large-scale repeat expansions. In this study, we performed high-resolution nuclear magnetic resonance spectroscopic investigations on DNA sequences containing two to five ATTCT repeats. Strikingly, we found the first two repeats of all these sequences well folded into highly compact minidumbbell (MDB) structures. The 3D solution structure of the sequence containing two ATTCT repeats was successfully determined, revealing the MDB comprises a regular TTCTA and a quasi TTCT/A pentaloops with extensive stabilizing loop-loop interactions. We further carried out in vitro primer extension assays to examine if the MDB formed in the primer could escape from the proofreading function of DNA polymerase. Results showed that when the MDB was formed at 5-bp or farther away from the priming site, it was able to escape from the proofreading by Klenow fragment of DNA polymerase I and thus retained in the primer. The intriguing structural findings bring about new insights into the origin of genetic instability in SCA10.

scholarly journals Comparison of the in Vitro Replication of the 7-(2-Oxoheptyl)-1,N2-etheno-2′-deoxyguanosine and 1,N2-Etheno-2′-deoxyguanosine Lesions bySulfolobus solfataricusP2 DNA Polymerase IV (Dpo4)

2010 ◽  
Vol 23 (8) ◽  
pp. 1330-1341 ◽  
Author(s):  
Plamen P. Christov ◽  
Katya V. Petrova ◽  
Ganesh Shanmugam ◽  
Ivan D. Kozekov ◽  
Albena Kozekova ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
In Vitro Replication ◽  
Dna Polymerase Iv
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