scholarly journals Derepression of Excision of Integrative and Potentially Conjugative Elements from Streptococcus thermophilus by DNA Damage Response: Implication of a cI-Related Repressor

2006 ◽  
Vol 189 (4) ◽  
pp. 1478-1481 ◽  
Author(s):  
Xavier Bellanger ◽  
Catherine Morel ◽  
Bernard Decaris ◽  
Gérard Guédon
Keyword(s):  
Dna Damage ◽  
Mitomycin C ◽  
Dna Damage Response ◽  
Streptococcus Thermophilus ◽  
Phage Lambda ◽  
Site Specific ◽  
Damage Response

ABSTRACT A DNA-damaging agent, mitomycin C, derepresses the site-specific excision of two integrative and potentially conjugative elements from Streptococcus thermophilus, ICESt1 and ICESt3. The regulation pathway involves a repressor related to phage lambda cI repressor. It could also involve a putative regulator related to another type of phage repressors, the “cI-like” repressors.

scholarly journals Site-specific DICER and DROSHA RNA products control the DNA-damage response

Nature ◽  
2012 ◽  
Vol 488 (7410) ◽  
pp. 231-235 ◽  
Author(s):  
Sofia Francia ◽  
Flavia Michelini ◽  
Alka Saxena ◽  
Dave Tang ◽  
Michiel de Hoon ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Damage Response ◽  
Site Specific ◽  
Damage Response

scholarly journals MicroRNA-128-3p regulates mitomycin C-induced DNA damage response in lung cancer cells through repressingSPTAN1

Oncotarget ◽  
2016 ◽  
Vol 8 (35) ◽  
pp. 58098-58107 ◽  
Author(s):  
Rui Zhang ◽  
Chang Liu ◽  
Yahan Niu ◽  
Ying Jing ◽  
Haiyang Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dna Damage ◽  
Cancer Cells ◽  
Mitomycin C ◽  
Dna Damage Response ◽  
Lung Cancer Cells ◽  
Damage Response

scholarly journals Site-Specific Phosphorylation of the DNA Damage Response Mediator Rad9 by Cyclin-Dependent Kinases Regulates Activation of Checkpoint Kinase 1

PLoS Genetics ◽  
2013 ◽  
Vol 9 (4) ◽  
pp. e1003310 ◽  
Author(s):  
Carla Manuela Abreu ◽  
Ramesh Kumar ◽  
Danielle Hamilton ◽  
Andrew William Dawdy ◽  
Kevin Creavin ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Damage Response ◽  
Site Specific ◽  
Checkpoint Kinase ◽  
Cyclin Dependent Kinases ◽  
Checkpoint Kinase 1 ◽  
Damage Response

scholarly journals Experimental strategies to achieve efficient targeted knock-in via tandem paired nicking

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Md. Lutfur Rahman ◽  
Toshinori Hyodo ◽  
Sivasundaram Karnan ◽  
Akinobu Ota ◽  
Muhammad Nazmul Hasan ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Damage Response ◽  
Life Science ◽  
Homologous Region ◽  
Site Specific ◽  
Damage Response ◽  
Reporter Systems ◽  
Endogenous Genes ◽  
Dna Strand ◽  
Experimental Strategies

AbstractTandem paired nicking (TPN) is a method of genome editing that enables precise and relatively efficient targeted knock-in without appreciable restraint by p53-mediated DNA damage response. TPN is initiated by introducing two site-specific nicks on the same DNA strand using Cas9 nickases in such a way that the nicks encompass the knock-in site and are located within a homologous region between a donor DNA and the genome. This nicking design results in the creation of two nicks on the donor DNA and two in the genome, leading to relatively efficient homology-directed recombination between these DNA fragments. In this study, we sought to identify the optimal design of TPN experiments that would improve the efficiency of targeted knock-in, using multiple reporter systems based on exogenous and endogenous genes. We found that efficient targeted knock-in via TPN is supported by the use of 1700–2000-bp donor DNAs, exactly 20-nt-long spacers predicted to be efficient in on-target cleavage, and tandem-paired Cas9 nickases nicking at positions close to each other. These findings will help establish a methodology for efficient and precise targeted knock-in based on TPN, which could broaden the applicability of targeted knock-in to various fields of life science.

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