scholarly journals Quantitative Real-Time PCR Detection of Toxic Nodularia Cyanobacteria in the Baltic Sea

2007 ◽  
Vol 73 (7) ◽  
pp. 2173-2179 ◽  
Author(s):  
Kerttu Koskenniemi ◽  
Christina Lyra ◽  
Pirjo Rajaniemi-Wacklin ◽  
Jouni Jokela ◽  
Kaarina Sivonen
Keyword(s):  
Baltic Sea ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Primers ◽  
Gene Copy ◽  
Quantitative Real Time Pcr ◽  
The Baltic Sea ◽  
Gene Copies ◽  
Qpcr Method ◽  
The Baltic

ABSTRACT A specific quantitative real-time PCR (qPCR) method was developed for the quantification of hepatotoxin nodularin-producing Nodularia, one of the main bloom-forming cyanobacteria in the Baltic Sea. Specific PCR primers were designed for subunit F of the nodularin synthetase gene (ndaF), which encodes the NdaF subunit of the nodularin synthetase gene complex needed for nodularin production. The qPCR method was applied to water samples (a total of 120 samples) collected from the Baltic Sea in July 2004. As few as 30 ndaF gene copies ml−1 of seawater could be detected, and thus, the method was very sensitive. The ndaF gene copy numbers and nodularin concentrations were shown to correlate in the Baltic seawater, indicating the constant production of nodularin by Nodularia. This qPCR method for the ndaF gene can be used for detailed studies of Nodularia blooms and their formation. ndaF gene copies and nodularin were detected mostly in the surface water but also in deeper water layers (down to 30 m). Toxic Nodularia blooms are not only horizontally but also vertically widely distributed, and thus, the Baltic fauna is extensively exposed to nodularin.

scholarly journals Meteo-Marine Parameters from Sentinel-1 SAR Imagery: Towards Near Real-Time Services for the Baltic Sea

2018 ◽  
Vol 10 (5) ◽  
pp. 757 ◽  
Author(s):  
Sander Rikka ◽  
Andrey Pleskachevsky ◽  
Sven Jacobsen ◽  
Victor Alari ◽  
Rivo Uiboupin
Keyword(s):  
Baltic Sea ◽  
Real Time ◽  
The Baltic Sea ◽  
Sar Imagery ◽  
The Baltic

scholarly journals Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report

2011 ◽  
Vol 56 (No. 6) ◽  
pp. 294-301 ◽  
Author(s):  
M. Blahutkova ◽  
P. Fictum ◽  
M. Skoric ◽  
B. Bezdekova ◽  
P. Jahn ◽  
...  
Keyword(s):  
Real Time ◽  
Mycobacterium Avium ◽  
Real Time Pcr ◽  
Intestinal Content ◽  
Mycobacterium Avium Subsp ◽  
Pathological Findings ◽  
Quantitative Real Time Pcr ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Qpcr Method

This report describes new possibilities for intravital and post mortem diagnosis of avian mycobacteriosis in horses using the quantitative real time PCR (qPCR) method. Using this method, Mycobacterium avium subsp. hominissuis was diagnosed in two sibling Fjord horses. In the first horse, M. a. hominissuis was detected by qPCR in numbers of 2.89 &times; 10<sup>5</sup> and 1.47 &times; 10<sup>4</sup> cells per 1 g of intestinal content and mesenteric lymph nodes, respectively; in the second horse, faeces and mesenteric lymph node samples showed numbers of 6.31 &times; 10<sup>5</sup> and 3.36 &times; 10<sup>6</sup> cells per 1 g of tissue, respectively. Another aim of this study was to comprehensively describe clinical and pathological findings in both animals.

Detection of Cryptosporidium parvum and Giardia lamblia in Water Supply by Quantitative Real-Time PCR

2012 ◽  
Vol 518-523 ◽  
pp. 3707-3711
Author(s):  
Peng Peng ◽  
Rui Bao Jia ◽  
Yu Mei Liu ◽  
Li Li
Keyword(s):  
Water Supply ◽  
Real Time ◽  
Cryptosporidium Parvum ◽  
Real Time Pcr ◽  
Giardia Lamblia ◽  
Pcr Primers ◽  
Quantitative Real Time Pcr ◽  
Rt Pcr ◽  
High Recovery ◽  
Pcr Method

Cryptosporidium parvum and Giardia lamblia are common pathogenic protozoa in water, which pose high risk to drinking water supply. In the present study, detection of C. parvum and G. lamblia was performed by quantitative real-time PCR (RT-PCR). Pairs of PCR primers were evaluated for the detection specificity to pathogenic C. parvum and G. lamblia. The recovery of the RT-PCR detection procedure was examined and high recovery rates (i.e., more than 45％ for C. parvum and more than 50％ for G. lamblia ) were achieved. The RT-PCR method was used to detect C. parvum and G. lamblia in a secondary water supply. The results indicated the potential application of the quantitative RT- PCR method in detection of C. parvum and G. lamblia in water supply.

scholarly journals Differential quantification of CYP2D6 gene copy number by four different quantitative real-time PCR assays

2010 ◽  
pp. 1 ◽  
Author(s):  
Anuradha Ramamoorthy ◽  
David A. Flockhart ◽  
Naoya Hosono ◽  
Michiaki Kubo ◽  
Yusuke Nakamura ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Quantitative Real Time Pcr ◽  
Cyp2d6 Gene ◽  
Pcr Assays

Detecting and monitoring endophytic colonization by Pseudomonas putida BP25 in black pepper (Piper nigrum L.) using quantitative real-time PCR

2017 ◽  
Vol 26 (1) ◽  
pp. 01 ◽  
Author(s):  
V N Agisha ◽  
S J Eapen ◽  
R S Bhai ◽  
A Kumar
Keyword(s):  
Pseudomonas Putida ◽  
Real Time ◽  
Real Time Pcr ◽  
Bacterial Colonization ◽  
Phytophthora Capsici ◽  
Pcr Primers ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Quantitative Real Time Pcr ◽  
Athelia Rolfsii

A quantitative real-time PCR assay was developed to quantify Pseudomonas putida BP25, an antagonistic endophyte against a broad range of pathogens in black pepper such as Phytophthora capsici, Colletotrichum gloeosporioides, Rhizoctonia solani, Gibberella moniliformis, Athelia rolfsii and a plant parasitic nematode, Radopholus similis. The real-time PCR primers were designed based on the16S rRNA sequences of P. putida strains and specificity of the primers was confirmed. The detection limit of the assay was found to be 1 pg. The assay detected and quantified the bacterial colonization in the roots at weekly intervals after inoculation. The P. putida DNA was quantified to be 0.4 ng in roots corresponding to 5.4 log10 CFU g-1 at 7th and 14th day after inoculation (DAI). A decline in endophyte population was observed during 21st and 28th DAI and the DNA concentration ranged from 3.7-4.6 pg corresponding to 3.4-3.5 log10 CFU g-1 of root. No amplification could be obtained in stem and leaf samples. The newly developed real-time PCR could be useful for detection, quantification and monitoring of endophytic P. putida BP25 in different plant tissues.  

GSTT1 and GSTM1 gene copy number analysis in paraffin-embedded tissue using quantitative real-time PCR

2008 ◽  
Vol 378 (2) ◽  
pp. 221-223 ◽  
Author(s):  
Naiara G. Bediaga ◽  
Miguel A. Alfonso-Sánchez ◽  
Mertxe de Renobales ◽  
Ana M. Rocandio ◽  
Marta Arroyo ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Copy Number Analysis ◽  
Quantitative Real Time Pcr ◽  
Gstm1 Gene
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