GSTT1 and GSTM1 gene copy number analysis in paraffin-embedded tissue using quantitative real-time PCR

2008 ◽  
Vol 378 (2) ◽  
pp. 221-223 ◽  
Author(s):  
Naiara G. Bediaga ◽  
Miguel A. Alfonso-Sánchez ◽  
Mertxe de Renobales ◽  
Ana M. Rocandio ◽  
Marta Arroyo ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Copy Number Analysis ◽  
Quantitative Real Time Pcr ◽  
Gstm1 Gene

scholarly journals Differential quantification of CYP2D6 gene copy number by four different quantitative real-time PCR assays

2010 ◽  
pp. 1 ◽  
Author(s):  
Anuradha Ramamoorthy ◽  
David A. Flockhart ◽  
Naoya Hosono ◽  
Michiaki Kubo ◽  
Yusuke Nakamura ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Quantitative Real Time Pcr ◽  
Cyp2d6 Gene ◽  
Pcr Assays

TaqMan real-time PCR quantification strategy of CYP2D6 gene copy number for the LightCycler 2.0

2009 ◽  
Vol 403 (1-2) ◽  
pp. 207-211 ◽  
Author(s):  
Duc L. Nguyen ◽  
Julia Staeker ◽  
Barbara Laika ◽  
Werner Steimer
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Cyp2d6 Gene

scholarly journals 27P Quantitative Analysis by Real-Time Pcr for ESR1 Gene Copy Number in Serum in Breast Cancer Samples

2012 ◽  
Vol 23 ◽  
pp. ii22
Author(s):  
J. Martinez-Galan ◽  
B. Torres-Torres ◽  
J.R. Delgado ◽  
M.I. Núñez ◽  
S. Ríos
Keyword(s):  
Breast Cancer ◽  
Quantitative Analysis ◽  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Esr1 Gene

A Simple Multiplex Real-Time PCR Methodology for the SMN1 Gene Copy Number Quantification

2009 ◽  
Vol 13 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Nadia Passon ◽  
Federico Pozzo ◽  
Cristiano Molinis ◽  
Elisa Bregant ◽  
Cinzia Gellera ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Smn1 Gene

scholarly journals Real-time PCR for the detection of precise transgene copy number in durum wheat

2011 ◽  
Vol 16 (4) ◽  
Author(s):  
Agata Gadaleta ◽  
Angelica Giancaspro ◽  
Maria Cardone ◽  
Antonio Blanco
Keyword(s):  
Durum Wheat ◽  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Gene Copy Number ◽  
Correlation Coefficients ◽  
Gene Copy ◽  
Transgene Copy Number ◽  
Locus Number ◽  
Wheat Lines

AbstractRecent results obtained in various crops indicate that real-time PCR could be a powerful tool for the detection and characterization of transgene locus structures. The determination of transgenic locus number through real-time PCR overcomes the problems linked to phenotypic segregation analysis (i.e. lack of detectable expression even when the transgenes are present) and can analyse hundreds of samples in a day, making it an efficient method for estimating gene copy number. Despite these advantages, many authors speak of “estimating” copy number by real-time PCR, and this is because the detection of a precise number of transgene depends on how well real-time PCR performs.This study was conducted to determine transgene copy number in transgenic wheat lines and to investigate potential variability in sensitivity and resolution of real-time chemistry by TaqMan probes. We have applied real-time PCR to a set of four transgenic durum wheat lines previously obtained. A total of 24 experiments (three experiments for two genes in each transgenic line) were conducted and standard curves were obtained from serial dilutions of the plasmids containing the genes of interest. The correlation coefficients ranged from 0.95 to 0.97. By using TaqMan quantitative real-time PCR we were able to detect 1 to 41 copies of transgenes per haploid genome in the DNA of homozygous T4 transformants. Although a slight variability was observed among PCR experiments, in our study we found real-time PCR to be a fast, sensitive and reliable method for the detection of transgene copy number in durum wheat, and a useful adjunct to Southern blot and FISH analyses to detect the presence of transgenic DNA in plant material.

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