scholarly journals Yersinia pseudotuberculosisPrevalence and Diversity in Wild Boars in Northeast Germany

2018 ◽  
Vol 84 (18) ◽  
Author(s):  
Marie Reinhardt ◽  
Jens Andre Hammerl ◽  
Katharina Kunz ◽  
Andrea Barac ◽  
Karsten Nöckler ◽  
...  
Keyword(s):  
Yersinia Pseudotuberculosis ◽  
Foodborne Pathogen ◽  
Virulence Plasmid ◽  
Whole Genome ◽  
Pathogenic Potential ◽  
Diagnostic Pcr ◽  
Content Type ◽  
Wild Boars ◽  
Contaminated Food ◽  
Human Infections

ABSTRACTIn this study, the prevalence ofYersinia pseudotuberculosisin wild boars in northeast Germany was determined. For that purpose, the tonsils of 503 wild boars were sampled. The presence ofY. pseudotuberculosiswas studied by diagnostic PCR. Positive samples were analyzed by cultural detection using a modified cold enrichment protocol. TenY. pseudotuberculosisisolates were obtained, which were characterized by biotyping, molecular serotyping, and multilocus sequence typing (MLST). In addition, whole-genome sequences and the antimicrobial susceptibility of the isolates were analyzed.Yersinia pseudotuberculosiswas isolated from male and female animals, most of which were younger than 1 year. A prevalence of 2% (10/503) was determined by cultural detection, while 6.4% (32/503) of the animals were positive by PCR. The isolates belonged to the biotypes 1 and 2 and serotypes O:1a (n= 7), O:1b (n= 2), and O:4a (n= 1). MLST analysis revealed three sequence types, ST9, ST23, and ST42. Except one isolate, all isolates revealed a strong resistance to colistin. The relationship of the isolates was studied by whole-genome sequencing demonstrating that they belonged to four clades, exhibiting five different pulsed-field gel electrophoresis (PFGE) restriction patterns and a diverse composition of virulence genes. Six isolates harbored the virulence plasmid pYV. Besides two isolates, all isolates containedailandinvgenes and a complete or incomplete high-pathogenicity island (HPI). None of them possessed a gene for the superantigen YPM. The study shows that variousY. pseudotuberculosisstrains exist in wild boars in northeast Germany, which may pose a risk to humans.IMPORTANCEYersinia pseudotuberculosisis a foodborne pathogen whose occurrence is poorly understood. One reason for this situation is the difficulty in isolating the species. The methods developed for the isolation ofYersinia enterocoliticaare not well suited forY. pseudotuberculosis. We therefore designed a protocol which enabled the isolation ofY. pseudotuberculosisfrom a relatively high proportion of PCR-positive wild boar tonsils. The study indicates that wild boars in northeast Germany may carry a variety ofY. pseudotuberculosisstrains, which differ in terms of their pathogenic potential and other properties. Since wild boars are widely distributed in German forests and even populate cities such as Berlin, they may transmit yersiniae to other animals and crop plants and may thus cause human infections through the consumption of contaminated food. Therefore, the prevalence ofY. pseudotuberculosisshould be determined also in other animals and regions to learn more about the natural reservoir of this species.

scholarly journals Complete Genome Sequences of 10 Yersinia pseudotuberculosis Isolates Recovered from Wild Boars in Germany

2018 ◽  
Vol 6 (19) ◽  
Author(s):  
Marie Reinhardt ◽  
Jens A. Hammerl ◽  
Stefan Hertwig
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Complete Genome ◽  
Yersinia Pseudotuberculosis ◽  
Draft Genome ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Wild Boars ◽  
Human Infections

ABSTRACT We report here the draft genome sequences of 10 Yersinia pseudotuberculosis isolates recovered from tonsils of wild boars hunted between 2015 and 2016 in Germany. Whole-genome sequencing and bioinformatic analyses were performed to assess the diversity of Y. pseudotuberculosis , which may result in human infections caused by the consumption of game meat.

scholarly journals Draft Whole-Genome Sequences of Seven Listeria monocytogenes Strains with Variations in Virulence and Stress Responses

2018 ◽  
Vol 7 (13) ◽  
Author(s):  
Yanhong Liu ◽  
Aixia Xu ◽  
Pina M. Fratamico ◽  
Christopher H. Sommers ◽  
Luca Rotundo ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Stress Responses ◽  
Draft Genome ◽  
Foodborne Pathogen ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type

Listeria monocytogenes is an important foodborne pathogen that causes listeriosis. Here, we report the draft genome sequences of seven L. monocytogenes strains isolated from food, environmental, and clinical sources.

scholarly journals Genome Sequence of Listeria monocytogenes Strain F4244, a 4b Serotype

2017 ◽  
Vol 5 (49) ◽  
Author(s):  
Taylor W. Bailey ◽  
Naila C. do Nascimento ◽  
Arun K. Bhunia
Keyword(s):  
Listeria Monocytogenes ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Genome Sequence ◽  
Illumina Sequencing ◽  
Foodborne Pathogen ◽  
Whole Genome ◽  
Content Type ◽  
Serotype 1 ◽  
Serotype 4B

ABSTRACT Listeria monocytogenes is an opportunistic invasive foodborne pathogen. Here, we performed whole-genome sequencing of L. monocytogenes strain F4244 (serotype 4b) using Illumina sequencing. The sequence showed 94.5% identity with strain F2365, serotype 4b, and 90.6% with EGD-e, serotype 1/2a.

scholarly journals High-Quality Whole-Genome Sequences for 77 Shiga Toxin-Producing Escherichia coli Strains Generated with PacBio Sequencing

2018 ◽  
Vol 6 (19) ◽  
Author(s):  
Pooja N. Patel ◽  
Rebecca L. Lindsey ◽  
Lisley Garcia-Toledo ◽  
Lori A. Rowe ◽  
Dhwani Batra ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Foodborne Pathogen ◽  
Severe Illness ◽  
Whole Genome ◽  
Genome Sequences ◽  
Pacbio Sequencing ◽  
High Quality ◽  
Content Type ◽  
Sequencing Platform

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) is an enteric foodborne pathogen that can cause mild to severe illness. Here, we report the availability of high-quality whole-genome sequences for 77 STEC strains generated using the PacBio sequencing platform.

scholarly journals Genotypic Characterization of Multidrug-Resistant Pseudomonas aeruginosa Using Whole-Genome Sequencing

2021 ◽  
Vol 10 (32) ◽  
Author(s):  
Baha Abdalhamid ◽  
Itidal Reslane ◽  
Emily Mccutchen ◽  
Peter C. Iwen
Keyword(s):  
Health Care ◽  
Pseudomonas Aeruginosa ◽  
Draft Genome ◽  
Multidrug Resistant ◽  
Whole Genome ◽  
Significant Morbidity ◽  
Genome Sequences ◽  
Content Type ◽  
Human Infections

Multidrug-resistant Pseudomonas aeruginosa is a serious threat worldwide causing health care-acquired infections and is associated with significant morbidity and mortality. This report describes the draft genome sequences of five multidrug-resistant Pseudomonas aeruginosa strains isolated from human infections.

scholarly journals Precision Food Safety: a Paradigm Shift in Detection and Control of Foodborne Pathogens

mSystems ◽  
2019 ◽  
Vol 4 (3) ◽  
Author(s):  
Jasna Kovac
Keyword(s):  
Food Safety ◽  
Foodborne Pathogens ◽  
Functional Characterization ◽  
Foodborne Pathogen ◽  
Genomic Data ◽  
Metagenomic Data ◽  
Whole Genome ◽  
Pathogenic Potential ◽  
Outbreak Investigations ◽  
Ongoing Surveillance

ABSTRACT The implementation of whole-genome sequencing in food safety has revolutionized foodborne pathogen tracking and outbreak investigations. The vast amounts of genomic data that are being produced through ongoing surveillance efforts continue advancing our understanding of pathogen diversity and genome biology. Produced genomic data are also supporting the use of metagenomics and metatranscriptomics for detection and functional characterization of microbiological hazards in foods and food processing environments. In addition to that, many studies have shown that metabolic and pathogenic potential, antimicrobial resistance, and other phenotypes relevant to food safety can be predicted from whole-genome sequences, omitting the need for multiple laboratory tests. Nevertheless, further work in the area of functional inference is necessary to enable accurate interpretation of functional information inferred from genomic and metagenomic data, as well as real-time detection and tracking of high-risk pathogen subtypes and microbiomes.

scholarly journals Whole-Genome Sequences of Listeria monocytogenes Sequence Type 6 Isolates Associated with a Large Foodborne Outbreak in South Africa, 2017 to 2018

2018 ◽  
Vol 6 (25) ◽  
Author(s):  
Mushal Allam ◽  
Nomsa Tau ◽  
Shannon L. Smouse ◽  
Phillip S. Mtshali ◽  
Florah Mnyameni ◽  
...  
Keyword(s):  
South Africa ◽  
Listeria Monocytogenes ◽  
Food Products ◽  
Sequence Type ◽  
Whole Genome ◽  
Foodborne Outbreak ◽  
Genome Sequences ◽  
Content Type ◽  
Contaminated Food

ABSTRACT We report whole-genome sequences for 10 Listeria monocytogenes sequence type 6 isolates associated with a large listeriosis outbreak in South Africa, which occurred over the period of 2017 to 2018. The possibility of listeriosis spreading beyond South Africa’s borders as a result of exported contaminated food products prompted us to make the genome sequences publicly available.

scholarly journals Genome Sequences of 13 Isolates of Salmonella enterica Serovar Typhimurium var. Copenhagen Obtained from Wild Pigeons in Canada

2018 ◽  
Vol 6 (20) ◽  
Author(s):  
Bridget Xie ◽  
Andrée Ann Dupras ◽  
Marc-Olivier Duceppe ◽  
Nooshin Fattahi-Ghazi ◽  
Lawrence Goodridge ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Virulence Plasmid ◽  
Avian Host ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Phage Types ◽  
Serovar Typhimurium

ABSTRACT Pigeon-adapted strains of Salmonella enterica serovar Typhimurium var. Copenhagen phage types 2 and 99 obtained from the provinces of Alberta, British Columbia, and Ontario, Canada, were analyzed using whole-genome sequencing. All isolates contained the Salmonella virulence plasmid despite the low pathogenicity of this lineage in their avian host.

scholarly journals Yersinia enterocolitica Isolates from Wild Boars Hunted in Lower Saxony, Germany

2015 ◽  
Vol 81 (14) ◽  
pp. 4835-4840 ◽  
Author(s):  
Alexandra von Altrock ◽  
Diana Seinige ◽  
Corinna Kehrenberg
Keyword(s):  
Yersinia Enterocolitica ◽  
Clinical Signs ◽  
Virulence Plasmid ◽  
Lower Saxony ◽  
Culture Methods ◽  
Hunting Season ◽  
Content Type ◽  
Wild Boars ◽  
Heat Stable ◽  
High Degree

ABSTRACTYersiniosis is strongly associated with the consumption of pork contaminated with enteropathogenicYersinia enterocolitica, which is harbored by domestic pigs without showing clinical signs of disease. In contrast to data onY. enterocoliticaisolated from conventionally reared swine, investigations into the occurrence ofY. enterocoliticain wild boars in Germany are rare. The objectives of the study were to get knowledge about these bacteria and their occurrence in wild boars hunted in northern Germany by isolation of the bacteria from the tonsils, identification of the bioserotypes, determination of selected virulence factors, macrorestriction analysis, multilocus sequence typing (MLST), and testing of antimicrobial susceptibility. Altogether, tonsils from 17.1% of 111 tested wild boars were positive forY. enterocoliticaby culture methods. All but two isolates belonged to biotype (BT) 1A, with the majority of isolates bearing aystBnucleotide sequence which was revealed to have 85% identity to internal regions ofY. enterocoliticaheat-stable enterotoxin type B genes. The remainingY. enterocoliticaisolates were identified to be BT 1B and did not carry the virulence plasmid. However, two BT 1A isolates carried theailgene. Macrorestriction analysis and results from MLST showed a high degree of genetic diversity of the isolates, although the region where the samples were taken was restricted to Lower Saxony, Germany, and wild boars were shot during one hunting season. In conclusion, mostY. enterocoliticaisolates from wild boars investigated in this study belonged to biotype 1A. EnteropathogenicY. enterocoliticabioserotypes 4/O:3 and 2/O:9, usually harbored by commercially raised pigs in Europe, could not be identified.

scholarly journals FSL J1-208, a Virulent Uncommon Phylogenetic Lineage IV Listeria monocytogenes Strain with a Small Chromosome Size and a Putative Virulence Plasmid Carrying Internalin-Like Genes

2012 ◽  
Vol 78 (6) ◽  
pp. 1876-1889 ◽  
Author(s):  
Henk C. den Bakker ◽  
Barbara M. Bowen ◽  
Lorraine D. Rodriguez-Rivera ◽  
Martin Wiedmann
Keyword(s):  
Genome Size ◽  
Virulence Plasmid ◽  
Chromosome Size ◽  
Total Size ◽  
Pathogenic Potential ◽  
Unique Type ◽  
Content Type ◽  
Small Genome ◽  
Phylogenetic Lineage ◽  
Lineage Iv

ABSTRACTThe bacterial genusListeriacontains both saprotrophic and facultative pathogenic species. A small genome size has been suggested to be associated with the loss of pathogenic potential ofL. welshimeriandL. seeligeri. In this paper we present data on the genome ofL. monocytogenesstrain FSL J1-208, a representative of phylogenetic lineage IV. Although this strain was isolated from a clinical case in a caprine host and has no decreased invasiveness in human intestinal epithelial cells, our analyses show that this strain has one of the smallestListeriachromosomes reported to date (2.78 Mb). The chromosome contains 2,772 protein-coding genes, including well-characterized virulence-associated genes, such asinlA,inlB, andinlCand the fullprfAgene cluster. The small genome size is mainly caused by the absence of prophages in the genome ofL. monocytogenesFSL J1-208, and further analyses showed that the total size of prophage-related regions is highly correlated to chromosome size in the genusListeria. L. monocytogenesFSL J1-208 carries a unique type of plasmid of approximately 80 kbp that does not carry genes annotated as being involved in resistance to antibiotics or heavy metals. The accessory genes in this plasmid belong to the internalin family, a family of virulence-associated proteins, and therefore this is the first report of a potential virulence plasmid in the genusListeria.

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