scholarly journals Antibiotic Resistance Patterns of Clinical Isolates of Serratia marcescens

1975 ◽  
Vol 7 (4) ◽  
pp. 396-399 ◽  
Author(s):  
R. C. Cooksey ◽  
E. R. Bannister ◽  
W. E. Farrar
Keyword(s):  
Antibiotic Resistance ◽  
Serratia Marcescens ◽  
Clinical Isolates ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

scholarly journals Resistance to Azithromycin and β-Lactam Antibiotics by Clinical Isolates of E. coli Isolated from Dhaka, Bangladesh

2019 ◽  
Vol 34 (2) ◽  
pp. 61-66
Author(s):  
Sunjukta Ahsan ◽  
Mayen Uddin ◽  
Juthika Mandal ◽  
Marufa Zerin Akhter
Keyword(s):  
Antibiotic Resistance ◽  
Efflux Pump ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Resistance Patterns ◽  
Ready Availability ◽  
Antibiotic Resistance Patterns

Antibiotic resistant E. coli are prevalent in Bangladesh. The indiscriminate use of antimicrobials and ready availability of over the counter drugs are responsible for this. This study was conducted to investigate the susceptibility of clinical Escherichia coli to the antibiotics Imipenem, Ceftriaxone, Ceftazidime and Azithromycin. Kirby-Bauer disk diffusion method was used to determine sensitivity to antimicrobials. Agar based assay was employed for the detection of efflux pumps. PCR was used amplify antibiotic resistance genes.All isolates were resistant to Ceftriaxone whereas most were sensitive to Imipenem. The MICs of Ceftazidime and Azithromycin ranged between 128 μg/ml and 256 μg/ml. The prevalence of ²-lactamase producers was 57.89 % with 36.84 % of the isolates exhibiting ESBL activity. No specific correlation could be found between plasmid sizes and antibiotic resistance patterns. Efflux pump was found to be involved in Azithromycin resistance in 63.15% of the isolates. The gene for phosphotransferase, mph(A) was the most common among the macrolide modifying genes, being present in 73.68% (14/19) of the isolates followed by both erm(A) anderm(C) esterases each present in 10.53% (2/19) isolates. This study concluded that clinical isolates of E. coli in Bangladesh could be resistant to multiple classes of antibiotics through different mechanisms of resistance. Bangladesh J Microbiol, Volume 34 Number 2 December 2017, pp 61-66

scholarly journals Incidence of ?-lactamase producing Escherichia coli amongst clinical isolates in Dhaka, Bangladesh

2016 ◽  
Vol 5 (1) ◽  
pp. 18-21
Author(s):  
Anindita Bhowmik ◽  
Sunjukta Ahsan ◽  
Marufa Zerin Akhtar ◽  
Md Shahidul Kabir
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Point Of View ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Drug Of Choice ◽  
Resistance Patterns ◽  
Lactam Antibiotic ◽  
Antibiotic Resistance Patterns

Escherichia coli is one of the most important clinical pathogens that demonstrate antibiotic resistance through their ? -lactamase activity. The present study reflected on the presence of ?-lactam antibiotic resistance in clinical samples. Antibiotic resistance patterns of 20 clinical isolates of Escherichia coli against ? -lactam antibiotics were investigated. These isolates were collected from a hospital in Dhaka city and MIC values were determined against Imipenem, Cefotaxime and Ceftriaxone. In case of Ceftriaxone the highest MIC was >4096 ?g/mL for 50% isolates with a minimum MIC of 1 ?g/ml for 10% isolates. In case of Cefotaxime, MIC values ranged between 1 ?g/ml and >4096 ?g/ml for 20% and 10% isolates, respectively. In contrast, 80% of the isolates had an MIC of Imipenem at 1 ?g/mL with only 5% isolate having MIC of 8 ?g/mL. Eight out of 20 (40%) isolates were ESBL producers, 14 (70%) were ? -lactamase producers. Two isolates exhibited ESBL activity against Cefotaxime and Ceftriaxone, 3 against only Cefotaxime and 3 against only Ceftriaxone. Ceftriaxone resistance in 10 randomly picked isolates was found to be stable for 21days in stability assay. Considering the findings of the present study, it appears that Imipenem still remains the drug of choice for E. coli infections; alternatively, combination therapy may be prescribed. In vitro stability of Ceftriaxone resistance raises concern from a public health point of view as this may aid antibiotic resistance transfer to other bacteria in the environment.Stamford Journal of Microbiology, Vol.5(1) 2015: 18-21

scholarly journals MULTIDRUG-RESISTANCE PATTERNS AND DETECTION OF PstS GENE IN CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA FROM NSUKKA, SOUTHEAST NIGERIA

2020 ◽  
pp. 115-119
Author(s):  
MARTINA C AGBO ◽  
IFEOMA M EZEONU ◽  
ANTHONY C IKE ◽  
CELESTINA C UGWU
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Multidrug Resistance ◽  
Test Strip ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Rdna Sequencing ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

Objective: This study was aimed to determine the antibiotic resistance patterns of clinical Pseudomonas aeruginosa isolates and to detect the presence of PstS gene. Methods: One hundred and ninety-two clinical isolates of P. aeruginosa were characterized using polymerase chain reaction (PCR) and 16S rDNA sequencing. Antibiotic resistance patterns were determined using the disk diffusion method, while the minimum inhibitory concentrations (MICs) of selected antibiotics against resistant isolates were determined by macro broth dilution and E-test strip methods. The resistant isolates were screened for the presence of PstS gene using PCR. Results: Of 192 clinical isolates of P. aeruginosa, 136 (70.83%) were resistant to at least two antibiotics. Of these, 135 (99%) could be classified as multidrug-resistant P. aeruginosa (MDR-PA), 63 (46%) were extensively drug-resistant (XDR-PA), while 38 (28%) were pandrug-resistant (PDR-PA). The isolates exhibited high level of resistance to cefotaxime and ticarcillin, and low levels of resistance to meropenem and imipenem. The MIC values for meropenem against the resistant isolates were generally <32 mg/L, while the values for other antibiotics ranged from 32 to >128 mg/L. Multiple antibiotic resistance indexes of the MDR-PA ranged from 0.27 to 0.91 and the most prevalent pattern of resistance was PiperacillinR – TicarcillinR – Piperacillin/TazobactamR – CefotaximeR – CeftazidimeR – GentamicnR – TobramycinR– CiprofloxacinR. About 50% of the resistant isolates possessed the PstS gene. Conclusions: The results confirmed the presence of XDR, PDRPA, and PstS gene in P. aeruginosa strains. There is an urgent need for healthcare practitioners to address the problem of multidrug resistance, by implementing a more rational and appropriate use of antibiotics.

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