scholarly journals Comparative In Vitro Antifungal Activity of Amphotericin B and Amphotericin B Methyl Ester

1975 ◽  
Vol 7 (1) ◽  
pp. 58-63 ◽  
Author(s):  
W. R. Howarth ◽  
R. P. Tewari ◽  
M. Solotorovsky
Keyword(s):  
Antifungal Activity ◽  
Methyl Ester ◽  
Amphotericin B ◽  
Amphotericin B Methyl Ester ◽  
In Vitro Antifungal Activity

scholarly journals Antifungal Activity of Flocculosin, a Novel Glycolipid Isolated from Pseudozyma flocculosa

2005 ◽  
Vol 49 (4) ◽  
pp. 1597-1599 ◽  
Author(s):  
Benjamin Mimee ◽  
Caroline Labbé ◽  
René Pelletier ◽  
Richard R. Bélanger
Keyword(s):  
Antifungal Activity ◽  
Amphotericin B ◽  
Cell Lines ◽  
Human Cell ◽  
Synergistic Activity ◽  
Human Cell Lines ◽  
Antifungal Properties ◽  
Pseudozyma Flocculosa ◽  
In Vitro Antifungal Activity

ABSTRACT Flocculosin, a glycolipid isolated from the yeast-like fungus Pseudozyma flocculosa, was investigated for in vitro antifungal activity. The compound displayed antifungal properties against several pathogenic yeasts. Synergistic activity was observed between flocculosin and amphotericin B, and no significant cytotoxicity was demonstrated when tested against human cell lines.

Amphotericin B in Lipid Emulsion: Stability, Compatibility, and In Vitro Antifungal Activity

1998 ◽  
Vol 42 (4) ◽  
pp. 762-766 ◽  
Author(s):  
Scott Walker ◽  
Sandra A. N. Tailor ◽  
Mark Lee ◽  
Lisa Louie ◽  
Marie Louie ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Amphotericin B ◽  
Clinical Laboratory ◽  
National Committee ◽  
Compatibility Study ◽  
The Stability ◽  
Few Data ◽  
Reference Strains ◽  
In Vitro Antifungal Activity

ABSTRACT Newer formulations of amphotericin B (AmB) complexed with liposomes or lipid suspensions have been developed. Preliminary studies have suggested that AmB in Intralipid (IL) may be as effective as, but less toxic than, conventional formulations of AmB, but few data are available regarding its stability, compatibility, or in vitro antifungal activity. A compatibility study was done to evaluate the effects of AmB concentrations in IL containing either 10 or 20% soybean oil. The effects of temperature, shaking, and AmB and IL concentrations on the stability of AmB-IL suspensions were analyzed by visual inspection and liquid chromatography. The in vitro antifungal activity of AmB-IL, compared to that of AmB alone against reference strains of Candida species was determined by using a broth macrodilution method in accordance with National Committee for Clinical Laboratory Standards guidelines (M27-T). Samples of AmB-IL which were lightly shaken retained more than 90% of the AmB concentration over 21 days when stored at either 4 or 23°C. Varying the AmB concentration did not appear to affect the stability of AmB-IL. However, a precipitate was formed when mixtures with more than 30% lipid as a proportion of the total volume were centrifuged. AmB-IL and AmB alone had similar in vitro antifungal activities against reference strains of yeasts. Further pharmacologic and clinical studies with AmB-IL are warranted, although AmB should not be combined with IL in concentrations capable of producing a precipitate.

Synergistic Antiviral Effect in vitro of Azidothymidine and Amphotericin B Methyl Ester in Combination on HIV infection

1992 ◽  
Vol 24 (1) ◽  
pp. 35-39 ◽  
Author(s):  
John-Erik Stig Hansen ◽  
Claus Nielsen ◽  
Annette Svenningsen ◽  
Niels Witzke ◽  
Lars R. Mathiesen
Keyword(s):  
Methyl Ester ◽  
Hiv Infection ◽  
Amphotericin B ◽  
Antiviral Effect ◽  
Amphotericin B Methyl Ester

scholarly journals Comparative in vitro studies on liposomal formulations of amphotericin B and its derivative, N-methyl-N-D-fructosyl amphotericin B methyl ester (MFAME).

2002 ◽  
Vol 49 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Barbara Cybulska ◽  
Karolina Kupczyk ◽  
Joanna Szlinder-Richert ◽  
Edward Borowski
Keyword(s):  
Methyl Ester ◽  
Amphotericin B ◽  
Mammalian Cells ◽  
Biological Properties ◽  
Antagonistic Effect ◽  
Molar Ratio ◽  
Selective Toxicity ◽  
Liposomal Formulations ◽  
Amphotericin B Methyl Ester

N-Methyl-N-D-fructosyl amphotericin B methyl ester (MFAME) is a semisynthetic derivative of the antifungal antibiotic amphotericin B (AMB). In contrast to the parent antibiotic, the derivative is characterised by low toxicity to mammalian cells and good solubility in water of its salts. Comparative studies on biological properties of free MFAME, AMB and their liposomal formulations were performed. To obtain liposomal forms, the antibiotics were incorporated into small unilamellar vesicles composed of dimyristoyl phosphatidylcholine (DMPC) and DMPC:cholesterol or ergosterol, 8:2 molar ratio. The effectivity of the liposomal and free forms of AMB and MFAME were compared by determination of fungistatic and fungicidal activity against Candida albicans ATCC 10261, potassium release from erythrocytes, and haemolysis. The results obtained indicate that in contrast to AMB, incorporation of MFAME into liposomes did not further improve its selective toxicity. Studies on the antagonistic effect of ergosterol and cholesterol on the antifungal activity of the antibiotics indicated that sterol interference was definitely less pronounced in the case of MFAME than in the case of AMB.

scholarly journals Comparative in vitro and in vivo evaluation of N-D-ornithyl amphotericin B methyl ester, amphotericin B methyl ester, and amphotericin B.

1987 ◽  
Vol 31 (11) ◽  
pp. 1756-1760 ◽  
Author(s):  
R M Parmegiani ◽  
D Loebenberg ◽  
B Antonacci ◽  
T Yarosh-Tomaine ◽  
R Scupp ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Amphotericin B ◽  
In Vivo Evaluation ◽  
Amphotericin B Methyl Ester

Comparison of the in vitro antifungal activity of free and liposome-encapsulated amphotericin B

1991 ◽  
Vol 10 (8) ◽  
pp. 665-668 ◽  
Author(s):  
E. Anaissie ◽  
V. Paetznick ◽  
R. Proffitt ◽  
J. Adler-Moore ◽  
G. P. Bodey
Keyword(s):  
Antifungal Activity ◽  
Amphotericin B ◽  
In Vitro Antifungal Activity

scholarly journals In vitro anti-mycoplasmal activity of amphotericin B methyl ester.

1976 ◽  
Vol 29 (6) ◽  
pp. 656-661 ◽  
Author(s):  
NEIL I. GOLDSTEIN ◽  
ARTHUR H. MCINTOSH ◽  
PAUL B. FISHER ◽  
KARL MARAMOROSCH ◽  
CARL P. SCHAFFNER
Keyword(s):  
Methyl Ester ◽  
Amphotericin B ◽  
Amphotericin B Methyl Ester

In vitro antifungal activity of amphotericin B and 11 comparators against Aspergillus terreus species complex

Mycoses ◽  
2017 ◽  
Vol 61 (2) ◽  
pp. 134-142 ◽  
Author(s):  
Afsane Vaezi ◽  
Hamed Fakhim ◽  
Amir Arastehfar ◽  
Tahereh Shokohi ◽  
Mohammad T. Hedayati ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Amphotericin B ◽  
Species Complex ◽  
Aspergillus Terreus ◽  
In Vitro Antifungal Activity
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