scholarly journals Identification of Drug Resistance Loci in Various Clinical Isolates of Neisseria gonorrhoeae

1977 ◽  
Vol 12 (3) ◽  
pp. 444-446 ◽  
Author(s):  
T. W. Maier ◽  
P. Warner ◽  
L. Zubryzycki ◽  
M. Chila
Keyword(s):  
Drug Resistance ◽  
Neisseria Gonorrhoeae ◽  
Clinical Isolates

Antimicrobial Drug Resistance and Resistance Factor Transfer among Clinical Isolates of Salmonellae in Iran

1990 ◽  
Vol 22 (2) ◽  
pp. 197-203 ◽  
Author(s):  
Ali A. Farhoudi-Moghaddam ◽  
Mohammad Katouli ◽  
Anis Jafari ◽  
Mohammad A. Bahavar ◽  
Mostafa Parsi ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antimicrobial Drug ◽  
Resistance Factor ◽  
Clinical Isolates ◽  
Antimicrobial Drug Resistance ◽  
Factor Transfer

scholarly journals Mosaic-Like Structure of Penicillin-Binding Protein 2 Gene (penA) in Clinical Isolates of Neisseria gonorrhoeae with Reduced Susceptibility to Cefixime

2002 ◽  
Vol 46 (12) ◽  
pp. 3744-3749 ◽  
Author(s):  
Satoshi Ameyama ◽  
Shoichi Onodera ◽  
Masahiro Takahata ◽  
Shinzaburo Minami ◽  
Nobuko Maki ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Neisseria Meningitidis ◽  
Binding Protein ◽  
Clinical Isolates ◽  
Penicillin Binding Protein ◽  
Gene Sequences ◽  
Neisseria Flavescens ◽  
Neisseria Perflava

ABSTRACT Neisseria gonorrhoeae strains with reduced susceptibility to cefixime (MICs, 0.25 to 0.5 μg/ml) were isolated from male urethritis patients in Tokyo, Japan, in 2000 and 2001. The resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, N. gonorrhoeae ATCC 19424, by transformation of the penicillin-binding protein 2 gene (penA) that had been amplified by PCR from a strain with reduced susceptibility to cefixime (MIC, 0.5 μg/ml). The sequences of penA in the strains with reduced susceptibilities to cefixime were different from those of other susceptible isolates and did not correspond to the reported N. gonorrhoeae penA gene sequences. Some regions in the transpeptidase-encoding domain in this penA gene were similar to those in the penA genes of Neisseria perflava (N. sicca), Neisseria cinerea, Neisseria flavescens, and Neisseria meningitidis. These results showed that a mosaic-like structure in the penA gene conferred reductions in the levels of susceptibility of N. gonorrhoeae to cephems and penicillin in a manner similar to that found for N. meningitidis and Streptococcus pneumoniae.

Resistance, molecular characterization and viability of Neisseria gonorrhoeae recent clinical isolates

2021 ◽  
Vol 156 (5) ◽  
pp. 249-250
Author(s):  
Elizabeth Calatrava-Hernández ◽  
Carla Foronda-García-Hidalgo ◽  
José Gutiérrez-Fernández
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Molecular Characterization ◽  
Clinical Isolates

scholarly journals Sequencing of Cytomegalovirus UL97 Gene for Genotypic Antiviral Resistance Testing

2001 ◽  
Vol 45 (10) ◽  
pp. 2775-2780 ◽  
Author(s):  
Nell S. Lurain ◽  
Adriana Weinberg ◽  
Clyde S. Crumpacker ◽  
Sunwen Chou
Keyword(s):  
Drug Resistance ◽  
Clinical Isolates ◽  
Resistance Testing ◽  
Virus Infectivity ◽  
Resistance Mutations ◽  
Sequence Identity ◽  
Complete Sequences ◽  
Immunosuppressed Patients ◽  
Ul97 Gene ◽  
Genotypic Assay

ABSTRACT The widespread use of ganciclovir (GCV) to treat cytomegalovirus (CMV) infections in immunosuppressed patients has led to the development of drug resistance. Phenotypic assays for CMV drug resistance are presently too time-consuming to be therapeutically useful. To support the development of genotypic assays for GCV resistance, the complete sequences of the UL97 phosphotransferase genes in 28 phenotypically GCV-sensitive CMV clinical isolates were determined. The gene was found to be highly conserved, with nucleotide sequence identity among strains ranging from 98.6 to 100% and amino acid sequence identity of >99%. Primers for a genotypic assay were designed to amplify codons 400 to 707, because all known UL97 mutations conferring drug resistance occur at three sites within this region. This part of the UL97 gene was amplified from over 50 clinical isolates, and two sequencing reactions for the coding strand were successfully used to identify GCV resistance mutations. This genotypic assay can be performed in 48 h using genomic DNA extracted from cell monolayers at very low levels of virus infectivity, thus rapidly providing therapeutically useful results.

Drug-Resistance Patterns of Clinical Isolates of Pseudomonas aeruginosa with Reference to Their Lipopolysaccharide Compositions

Chemotherapy ◽  
1997 ◽  
Vol 43 (5) ◽  
pp. 323-331 ◽  
Author(s):  
Miyuki Hasegawa ◽  
Intetsu Kobayashi ◽  
Takeshi Saika ◽  
Minoru Nishida
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistance ◽  
Clinical Isolates ◽  
Resistance Patterns

scholarly journals Prevalence of Virulence Genes and Drug Resistance Profiles of Pseudomonas aeruginosa Isolated From Clinical Specimens

2021 ◽  
Vol 14 (8) ◽  
Author(s):  
Seyed Ali Bazghandi ◽  
Mohsen Arzanlou ◽  
Hadi Peeridogaheh ◽  
Hamid Vaez ◽  
Amirhossein Sahebkar ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Clinical Specimens ◽  
Resistance Rates

Background: Drug resistance and virulence genes are two key factors for the colonization of Pseudomonas aeruginosa in settings with high antibiotic pressure, such as hospitals, and the development of hospital-acquired infections. Objectives: The objective of this study was to investigate the prevalence of drug resistance and virulence gene profiles in clinical isolates of P. aeruginosa in Ardabil, Iran. Methods: A total of 84 P. aeruginosa isolates were collected from clinical specimens of Ardabil hospitals and confirmed using laboratory standard tests. The disk diffusion method was used for antibiotic susceptibility testing and polymerase chain reaction (PCR) for the identification of P. aeruginosa virulence genes. Results: The highest and the lowest antibiotic resistance rates of P. aeruginosa strains were against ticarcillin-clavulanate (94%) and doripenem (33.3%), respectively. In addition, the frequency of multidrug-resistant (MDR) P. aeruginosa was 55.9%. The prevalence of virulence factor genes was as follows: algD 84.5%, lasB 86.9%, plcH 86.9%, plcN 86.9%, exoU 56%, exoS 51.2%, toxA 81%, nan1 13.1%, and pilB 33.3%. A significant association was observed between resistance to some antibiotics and the prevalence of virulence genes in P. aeruginosa. Conclusions: Our results revealed a high prevalence of antibiotic resistance, especially MDR, and virulence-associated genes in clinical isolates of P. aeruginosa in Ardabil hospitals. Owing to the low resistance rates against doripenem, gentamicin, and tobramycin, these antibiotics are recommended for the treatment of infections caused by highly resistant and virulent P. aeruginosa strains.

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