scholarly journals High Prevalence ofblaNDM-1Carbapenemase-Encoding Gene and 16S rRNAarmAMethyltransferase Gene among Acinetobacter baumannii Clinical Isolates in Egypt

2015 ◽  
Vol 59 (6) ◽  
pp. 3602-3605 ◽  
Author(s):  
Mohamed Abd El-Gawad El-Sayed-Ahmed ◽  
Magdy Ali Amin ◽  
Wael Mustafa Tawakol ◽  
Lotfi Loucif ◽  
Sofiane Bakour ◽  
...  
Keyword(s):  
16S Rrna ◽  
Acinetobacter Baumannii ◽  
Clinical Isolates ◽  
Content Type ◽  
High Prevalence ◽  
Distinct Sequence ◽  
Encoding Gene ◽  
First Time ◽  
Sequence Types ◽  
16S Rrna Methylase

ABSTRACTThe main objective of this study was to decipher the molecular mechanism of resistance to carbapenems and aminoglycosides in a large series of 150Acinetobacter baumanniiclinical isolates collected from July 2012 to September 2013 in Egypt. We report for the first time the emergence ofblaNDM-1and the cooccurrence of 16S rRNA methylasearmAwithblaNDM-1andblaOXA-23in Egyptian hospitals. Multilocus sequence typing identified 27 distinct sequence types, 11 of which were novel.

Characterization of Acinetobacter baumannii Clinical Isolates Carrying blaOXA-23 Carbapenemase and 16S rRNA Methylase armA genes in Yemen

2014 ◽  
Vol 20 (6) ◽  
pp. 604-609 ◽  
Author(s):  
Sofiane Bakour ◽  
Samer Ahmed Alsharapy ◽  
Abdelaziz Touati ◽  
Jean-Marc Rolain
Keyword(s):  
16S Rrna ◽  
Acinetobacter Baumannii ◽  
Clinical Isolates ◽  
16S Rrna Methylase

scholarly journals Frequency of 16S rRNA Methylase and Aminoglycoside-Modifying Enzyme Genes among Clinical Isolates of Acinetobacter baumannii in Iran

2017 ◽  
Vol 12 (4) ◽  
pp. 329-338 ◽  
Author(s):  
Mehrdad Gholami ◽  
Mohammadreza Haghshenas ◽  
Mona Moshiri ◽  
Shbnam Razavi ◽  
Abazar Pournajaf ◽  
...  
Keyword(s):  
16S Rrna ◽  
Acinetobacter Baumannii ◽  
Clinical Isolates ◽  
16S Rrna Methylase

Clonal Dissemination of Clinical Isolates of Acinetobacter baumannii Carriers of 16S rRNA Methylase Genes in an Oncological Hospital in Recife, Brazil

2019 ◽  
Vol 77 (1) ◽  
pp. 32-39 ◽  
Author(s):  
Jussyêgles Niedja da Paz Pereira ◽  
Carlos Alberto das Neves de Andrade ◽  
Jailton Lobo da Costa Lima ◽  
Reginaldo Gonçalves de Lima Neto ◽  
Paulo Sérgio Ramos de Araújo ◽  
...  
Keyword(s):  
16S Rrna ◽  
Acinetobacter Baumannii ◽  
Clinical Isolates ◽  
16S Rrna Methylase

scholarly journals Highly multidrug-resistant Morganella morganii clinical isolates from Nepal co-producing NDM-type metallo-β-lactamases and the 16S rRNA methylase ArmA

2020 ◽  
Vol 69 (4) ◽  
pp. 572-575
Author(s):  
Shovita Shrestha ◽  
Tatsuya Tada ◽  
Jatan B. Sherchan ◽  
Hiroki Uchida ◽  
Tomomi Hishinuma ◽  
...  
Keyword(s):  
16S Rrna ◽  
Control Systems ◽  
Type Species ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Morganella Morganii ◽  
Content Type ◽  
Link Type ◽  
16S Rrna Methylase ◽  
Multiple Antibiotics

Morganella morganii can harbour extended-spectrum β-lactamases and carbapenemases, resulting in increased resistance to multiple antibiotics and a high mortality rate. This study describes the emergence of highly multidrug-resistant clinical isolates of M. morganii from Nepal co-producing NDM-type metallo-β-lactamases, including NDM-1 and NDM-5, and the 16S rRNA methylase ArmA. This is the first report of M. morganii clinical isolates from Nepal co-producing NDM-1/-5 and ArmA. It is important to establish infection control systems and effective treatments against multidrug-resistant M. morganii .

scholarly journals Klebsiella pneumoniae Carbapenemase (KPC)-Producing K. pneumoniae at a Single Institution: Insights into Endemicity from Whole-Genome Sequencing

2015 ◽  
Vol 59 (3) ◽  
pp. 1656-1663 ◽  
Author(s):  
Amy J. Mathers ◽  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Louise Pankhurst ◽  
Adam Giess ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Clinical Isolates ◽  
Whole Genome ◽  
Single Institution ◽  
Content Type ◽  
Klebsiella Pneumoniae Carbapenemase ◽  
Distinct Sequence ◽  
Sequence Types

ABSTRACTThe global emergence ofKlebsiella pneumoniaecarbapenemase-producingK. pneumoniae(KPC-Kp) multilocus sequence type ST258 is widely recognized. Less is known about the molecular and epidemiological details of non-ST258K. pneumoniaein the setting of an outbreak mediated by an endemic plasmid. We describe the interplay ofblaKPCplasmids andK. pneumoniaestrains and their relationship to the location of acquisition in a U.S. health care institution. Whole-genome sequencing (WGS) analysis was applied to KPC-Kpclinical isolates collected from a single institution over 5 years following the introduction ofblaKPCin August 2007, as well as two plasmid transformants. KPC-Kpfrom 37 patients yielded 16 distinct sequence types (STs). Two novel conjugativeblaKPCplasmids (pKPC_UVA01 and pKPC_UVA02), carried by the hospital index case, accounted for the presence ofblaKPCin 21/37 (57%) subsequent cases. Thirteen (35%) isolates represented an emergent lineage, ST941, which contained pKPC_UVA01 in 5/13 (38%) and pKPC_UVA02 in 6/13 (46%) cases. Seven (19%) isolates were the epidemic KPC-Kpstrain, ST258, mostly imported from elsewhere and not carrying pKPC_UVA01 or pKPC_UVA02. Using WGS-based analysis of clinical isolates and plasmid transformants, we demonstrate the unexpected dispersal ofblaKPCto many non-ST258 lineages in a hospital through spread of at least two novelblaKPCplasmids. In contrast, ST258 KPC-Kpwas imported into the institution on numerous occasions, with otherblaKPCplasmid vectors and without sustained transmission. Instead, a newly recognized KPC-Kpstrain, ST941, became associated with both novelblaKPCplasmids and spread locally, making it a future candidate for clinical persistence and dissemination.

scholarly journals Emergence of Various NDM-Type-Metallo-β-Lactamase-Producing Escherichia coli Clinical Isolates in Nepal

2017 ◽  
Vol 61 (12) ◽  
Author(s):  
Basudha Shrestha ◽  
Tatsuya Tada ◽  
Kayo Shimada ◽  
Shovita Shrestha ◽  
Hiroshi Ohara ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Clinical Isolates ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Methylase Gene ◽  
16S Rrna Methylase

ABSTRACT Of 250 clinical isolates of Escherichia coli obtained in Nepal, 38 were carbapenem resistant, with MICs of imipenem or meropenem of ≥4 μg/ml. All 38 isolates harbored the following bla NDMs: bla NDM-1, bla NDM-3, bla NDM-4, bla NDM-5, bla NDM-7, bla NDM-12, and bla NDM-13. Most of these isolates also harbored the 16S rRNA methylase gene(s) armA, rmtB, and/or rmtC.

scholarly journals High Prevalence of 16S rRNA methylase genes High Prevalence of 16S rRNA methylase genes Among Carbapenem-resistant Hypervirulent Klebsiella pneumoniae Isolates in China

2019 ◽  
Author(s):  
Wenjian Liao ◽  
Dan Li ◽  
Dan Dan Wei ◽  
Fang-lin Du ◽  
Dan Long ◽  
...  
Keyword(s):  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Clinical Isolates ◽  
Aminoglycoside Resistance ◽  
Pfge Typing ◽  
Carbapenem Resistant ◽  
Carbapenemase Gene ◽  
High Prevalence ◽  
High Level ◽  
16S Rrna Methylase

Abstract Background : the existence of 16S rRNA methylase genes would increase treatment difficulty of patients infected with CR-hvKP strains, this study was aimed to testify the prevalence of the 16S rRNA methylase genes genes in the CR-hvKP strains in China.Methods : Thirty-nine carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) isolates collected from a Chinese hospital during the whole year of 2018 were evaluated to characterize the prevalence of 16S rRNA methylase genes. Results : In tatal 66.7% (26/39) of the CR-hvKP isolates were found to carry 16S rRNA methylase genes, and the most frequently detected gene was armA (11,42.3%), followed by rmtB (8,30.8%),and 7 CR-hvKP strains were found to carry both armA and rmtB (26.9%). All the clinical isolates were found to carry at least one carbapenemase gene,with KPC-2 (79.5%,31/39), NDM-1 (10.3%,4/39), and cocarrying KPC-2 and NDM-1 (10.3%,4/39). A total of 89.7% (35/39) isolates carried ESBL genes, including 61.5% (24/39) blaSHV-1 ,71.8% (28/39) blaTEM-1 and 89.7% (35/39) blaCTX-M-1 4. All except four isolates (89.7%,35/39) harbored PMQR genes,with qnrS (82.1%,32/39), aac(6’)-Ib-cr (79.5%,31/39), qnrB (2.6%,1/39).All the 16S rRNA methylase genes-positive CR-hvKP strains were firstly found to cocarry carbapenemase genes, ESBL genes and PMQR genes simultaneously. The most prevalent virulence genes were rmpA2 and entB (100%, 39/39),followed by silS (97.4%, 38/39), ybtS (94.9%, 37/39), iutA (92.3%, 36/39), kpn (92.3%, 36/39), rmpA (87.2%, 34/39), terW (84.6%, 33/39), aerobactin (23.1%, 9/39), repA (17.9%, 7/39), magA (10.3%, 4/39), kfuB C (10.3%, 4/39), w ca G (10.3%, 4/39), allS (10.3%, 4/39). Multilocus sequence typing (MLST) analysis assigned the 39 CR-hvKP isolates into 4 sequence types (STs), with ST11 encompassing 79.5% of the strains. Pulsed field gel electrophoresis (PFGE) typing showed that strains closely related by MLST clustered in major PFGE clusters, of which cluster A accounts for 31 ST11 isolates.The analysis of the transconjugants showed a high-level aminoglycoside resistance and a popular cotransfer of bla KPC-2 with the 16S rRNA methylase genes.Conclusions : 16S rRNA methylase genes are highly prevalent in CR-hvKP clinical isolates especially for ST11, it is therefore critical to continuously monitor the 16S rRNA methylase-producing CR-hvKP epidemiology and minimize potential risks from aminoglycoside -resistant CR-hvKP.

scholarly journals Molecular Epidemiology and Mechanisms of Tigecycline Resistance in Clinical Isolates of Acinetobacter baumannii from a Chinese University Hospital

2013 ◽  
Vol 58 (1) ◽  
pp. 297-303 ◽  
Author(s):  
Mei Deng ◽  
Man-Hua Zhu ◽  
Jun-Jie Li ◽  
Sheng Bi ◽  
Zi-Ke Sheng ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Efflux Pump ◽  
Infectious Agent ◽  
Clinical Isolates ◽  
University Hospital ◽  
Resistance Pattern ◽  
Content Type ◽  
Active Efflux ◽  
Carbonyl Cyanide ◽  
Sequence Types

ABSTRACTBecause of its remarkable ability to acquire antibiotic resistance and to survive in nosocomial environments,Acinetobacter baumanniihas become a significant nosocomial infectious agent worldwide. Tigecycline is one of the few therapeutic options for treating infections caused byA. baumanniiisolates. However, tigecycline resistance has increasingly been reported. Our aim was to assess the prevalence and characteristics of efflux-based tigecycline resistance in clinical isolates ofA. baumanniicollected from a hospital in China. A total of 74A. baumanniiisolates, including 64 tigecycline-nonsusceptibleA. baumannii(TNAB) and 10 tigecycline-susceptibleA. baumannii(TSAB) isolates, were analyzed. The majority of them were determined to be positive foradeABC,adeRS,adeIJK, andabeM, while theadeEgene was found in only one TSAB isolate. Compared with the levels in TSAB isolates, the mean expression levels ofadeB,adeJ,adeG, andabeMin TNAB isolates were observed to increase 29-, 3-, 0.7-, and 1-fold, respectively. The efflux pump inhibitors (EPIs) phenyl-arginine-β-naphthylamide (PAβN) and carbonyl cyanide 3-chlorophenylhydrazone (CCCP) could partially reverse the resistance pattern of tigecycline. Moreover, thetetX1gene was detected in 12 (18.8%) TNAB isolates. To our knowledge, this is the first report of thetetX1gene being detected inA. baumanniiisolates. ST208 and ST191, which both clustered into clonal complex 92 (CC92), were the predominant sequence types (STs). This study showed that the active efflux pump AdeABC appeared to play important roles in the tigecycline resistance ofA. baumannii. The dissemination of TNAB isolates in our hospital is attributable mainly to the spread of CC92.

scholarly journals High Prevalence of Metallo-β-Lactamase and 16S rRNA Methylase Coproduction among Imipenem-Resistant Pseudomonas aeruginosa Isolates in Brazil

2007 ◽  
Vol 51 (9) ◽  
pp. 3388-3390 ◽  
Author(s):  
Yohei Doi ◽  
Angela C. R. Ghilardi ◽  
Jennifer Adams ◽  
Doroti de Oliveira Garcia ◽  
David L. Paterson
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Geographic Area ◽  
Clinical Isolates ◽  
High Prevalence ◽  
16S Rrna Methylase

ABSTRACT Rates of metallo-β-lactamase and 16S rRNA methylase production were investigated in 51 imipenem-resistant Pseudomonas aeruginosa clinical isolates collected from hospitals in São Paulo, Brazil. Of them, 57% and 75% produced SPM-1 and RmtD, respectively. Of note, 51% produced both enzymes, suggesting that their coproduction is already common in this geographic area.

Sign in / Sign up

Export Citation Format

Share Document