scholarly journals High Prevalence of Metallo-β-Lactamase and 16S rRNA Methylase Coproduction among Imipenem-Resistant Pseudomonas aeruginosa Isolates in Brazil

2007 ◽  
Vol 51 (9) ◽  
pp. 3388-3390 ◽  
Author(s):  
Yohei Doi ◽  
Angela C. R. Ghilardi ◽  
Jennifer Adams ◽  
Doroti de Oliveira Garcia ◽  
David L. Paterson
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Geographic Area ◽  
Clinical Isolates ◽  
High Prevalence ◽  
16S Rrna Methylase

ABSTRACT Rates of metallo-β-lactamase and 16S rRNA methylase production were investigated in 51 imipenem-resistant Pseudomonas aeruginosa clinical isolates collected from hospitals in São Paulo, Brazil. Of them, 57% and 75% produced SPM-1 and RmtD, respectively. Of note, 51% produced both enzymes, suggesting that their coproduction is already common in this geographic area.

scholarly journals High Prevalence of 16S rRNA methylase genes High Prevalence of 16S rRNA methylase genes Among Carbapenem-resistant Hypervirulent Klebsiella pneumoniae Isolates in China

2019 ◽  
Author(s):  
Wenjian Liao ◽  
Dan Li ◽  
Dan Dan Wei ◽  
Fang-lin Du ◽  
Dan Long ◽  
...  
Keyword(s):  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
Clinical Isolates ◽  
Aminoglycoside Resistance ◽  
Pfge Typing ◽  
Carbapenem Resistant ◽  
Carbapenemase Gene ◽  
High Prevalence ◽  
High Level ◽  
16S Rrna Methylase

Abstract Background : the existence of 16S rRNA methylase genes would increase treatment difficulty of patients infected with CR-hvKP strains, this study was aimed to testify the prevalence of the 16S rRNA methylase genes genes in the CR-hvKP strains in China.Methods : Thirty-nine carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) isolates collected from a Chinese hospital during the whole year of 2018 were evaluated to characterize the prevalence of 16S rRNA methylase genes. Results : In tatal 66.7% (26/39) of the CR-hvKP isolates were found to carry 16S rRNA methylase genes, and the most frequently detected gene was armA (11,42.3%), followed by rmtB (8,30.8%),and 7 CR-hvKP strains were found to carry both armA and rmtB (26.9%). All the clinical isolates were found to carry at least one carbapenemase gene,with KPC-2 (79.5%,31/39), NDM-1 (10.3%,4/39), and cocarrying KPC-2 and NDM-1 (10.3%,4/39). A total of 89.7% (35/39) isolates carried ESBL genes, including 61.5% (24/39) blaSHV-1 ,71.8% (28/39) blaTEM-1 and 89.7% (35/39) blaCTX-M-1 4. All except four isolates (89.7%,35/39) harbored PMQR genes,with qnrS (82.1%,32/39), aac(6’)-Ib-cr (79.5%,31/39), qnrB (2.6%,1/39).All the 16S rRNA methylase genes-positive CR-hvKP strains were firstly found to cocarry carbapenemase genes, ESBL genes and PMQR genes simultaneously. The most prevalent virulence genes were rmpA2 and entB (100%, 39/39),followed by silS (97.4%, 38/39), ybtS (94.9%, 37/39), iutA (92.3%, 36/39), kpn (92.3%, 36/39), rmpA (87.2%, 34/39), terW (84.6%, 33/39), aerobactin (23.1%, 9/39), repA (17.9%, 7/39), magA (10.3%, 4/39), kfuB C (10.3%, 4/39), w ca G (10.3%, 4/39), allS (10.3%, 4/39). Multilocus sequence typing (MLST) analysis assigned the 39 CR-hvKP isolates into 4 sequence types (STs), with ST11 encompassing 79.5% of the strains. Pulsed field gel electrophoresis (PFGE) typing showed that strains closely related by MLST clustered in major PFGE clusters, of which cluster A accounts for 31 ST11 isolates.The analysis of the transconjugants showed a high-level aminoglycoside resistance and a popular cotransfer of bla KPC-2 with the 16S rRNA methylase genes.Conclusions : 16S rRNA methylase genes are highly prevalent in CR-hvKP clinical isolates especially for ST11, it is therefore critical to continuously monitor the 16S rRNA methylase-producing CR-hvKP epidemiology and minimize potential risks from aminoglycoside -resistant CR-hvKP.

scholarly journals High Prevalence ofblaNDM-1Carbapenemase-Encoding Gene and 16S rRNAarmAMethyltransferase Gene among Acinetobacter baumannii Clinical Isolates in Egypt

2015 ◽  
Vol 59 (6) ◽  
pp. 3602-3605 ◽  
Author(s):  
Mohamed Abd El-Gawad El-Sayed-Ahmed ◽  
Magdy Ali Amin ◽  
Wael Mustafa Tawakol ◽  
Lotfi Loucif ◽  
Sofiane Bakour ◽  
...  
Keyword(s):  
16S Rrna ◽  
Acinetobacter Baumannii ◽  
Clinical Isolates ◽  
Content Type ◽  
High Prevalence ◽  
Distinct Sequence ◽  
Encoding Gene ◽  
First Time ◽  
Sequence Types ◽  
16S Rrna Methylase

ABSTRACTThe main objective of this study was to decipher the molecular mechanism of resistance to carbapenems and aminoglycosides in a large series of 150Acinetobacter baumanniiclinical isolates collected from July 2012 to September 2013 in Egypt. We report for the first time the emergence ofblaNDM-1and the cooccurrence of 16S rRNA methylasearmAwithblaNDM-1andblaOXA-23in Egyptian hospitals. Multilocus sequence typing identified 27 distinct sequence types, 11 of which were novel.

Characterization of Acinetobacter baumannii Clinical Isolates Carrying blaOXA-23 Carbapenemase and 16S rRNA Methylase armA genes in Yemen

2014 ◽  
Vol 20 (6) ◽  
pp. 604-609 ◽  
Author(s):  
Sofiane Bakour ◽  
Samer Ahmed Alsharapy ◽  
Abdelaziz Touati ◽  
Jean-Marc Rolain
Keyword(s):  
16S Rrna ◽  
Acinetobacter Baumannii ◽  
Clinical Isolates ◽  
16S Rrna Methylase

Characterization of 16S rRNA methylase genes in Enterobacterales and Pseudomonas aeruginosa in Athens Metropolitan area, 2015–2016

2020 ◽  
Vol 40 (1) ◽  
pp. 111-121
Author(s):  
Konstantina Nafplioti ◽  
Maria Souli ◽  
Panagiota Adamou ◽  
Eleni Moraitou ◽  
Panagiota Giannopoulou ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Metropolitan Area ◽  
16S Rrna Methylase

scholarly journals Pseudomonas aeruginosa Clinical Isolates in Nepal Coproducing Metallo-β-Lactamases and 16S rRNA Methyltransferases

2017 ◽  
Vol 61 (9) ◽  
Author(s):  
Tatsuya Tada ◽  
Kayo Shimada ◽  
Kazuhito Satou ◽  
Takashi Hirano ◽  
Bharat M. Pokhrel ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Content Type ◽  
First Report ◽  
Genes Encoding

ABSTRACT A total of 11 multidrug-resistant Pseudomonas aeruginosa clinical isolates were obtained in Nepal. Four of these isolates harbored genes encoding one or more carbapenemases (DIM-1, NDM-1, and/or VIM-2), and five harbored genes encoding a 16S rRNA methyltransferase (RmtB4 or RmtF2). A novel RmtF variant, RmtF2, had a substitution (K65E) compared with the same gene in RmtF. To our knowledge, this is the first report describing carbapenemase- and 16S rRNA methyltransferase-coproducing P. aeruginosa clinical isolates in Nepal.

scholarly journals Prevalence of 16S rRNA methylase genes among β-lactamase-producing Enterobacteriaceae clinical isolates in Saudi Arabia

2014 ◽  
Vol 9 (1) ◽  
pp. 24432 ◽  
Author(s):  
Yazeed A. Al Sheikh ◽  
Mohammed Ali M. Marie ◽  
James John ◽  
Lakshmana Gowda Krishnappa ◽  
Khaled Homoud M. Dabwab
Keyword(s):  
Saudi Arabia ◽  
16S Rrna ◽  
Clinical Isolates ◽  
16S Rrna Methylase

scholarly journals Emergence of 16S rRNA methylase-producing Acinetobacter baumannii and Pseudomonas aeruginosa isolates in hospitals in Vietnam

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Tatsuya Tada ◽  
Tohru Miyoshi-Akiyama ◽  
Yasuyuki Kato ◽  
Norio Ohmagari ◽  
Nozomi Takeshita ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
Acinetobacter Baumannii ◽  
16S Rrna Methylase

Emergence of 16S rRNA methylase gene armA and cocarriage of blaIMP-1 in Pseudomonas aeruginosa isolates from South Korea

2010 ◽  
Vol 68 (4) ◽  
pp. 468-470 ◽  
Author(s):  
Mamata Gurung ◽  
Dong Chan Moon ◽  
Migma Dorji Tamang ◽  
Jungmin Kim ◽  
Yoo Chul Lee ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
16S Rrna ◽  
South Korea ◽  
Methylase Gene ◽  
16S Rrna Methylase
Sign in / Sign up

Export Citation Format

Share Document