scholarly journals The β-Defensin Gallinacin-6 Is Expressed in the Chicken Digestive Tract and Has Antimicrobial Activity against Food-Borne Pathogens

2006 ◽  
Vol 51 (3) ◽  
pp. 912-922 ◽  
Author(s):  
Albert van Dijk ◽  
Edwin J. A. Veldhuizen ◽  
Stefanie I. C. Kalkhove ◽  
Johanna L. M. Tjeerdsma-van Bokhoven ◽  
Roland A. Romijn ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Digestive Tract ◽  
Clostridium Perfringens ◽  
Developed Countries ◽  
High Expression ◽  
Upstream Region ◽  
Pcr Analysis ◽  
Nuclear Factor ◽  
Food Borne Pathogens ◽  
Food Borne

ABSTRACT Food-borne pathogens are responsible for most cases of food poisoning in developed countries and are often associated with poultry products, including chicken. Little is known about the role of β-defensins in the chicken digestive tract and their efficacy. In this study, the expression of chicken β-defensin gallinacin-6 (Gal-6) and its antimicrobial activity against food-borne pathogens were investigated. Reverse transcription-PCR analysis showed high expression of Gal-6 mRNA in the esophagus and crop, moderate expression in the glandular stomach, and low expression throughout the intestinal tract. Putative transcription factor binding sites for nuclear factor kappa beta, activator protein 1, and nuclear factor interleukin-6 were found in the Gal-6 gene upstream region, which suggests a possible inducible nature of the Gal-6 gene. In colony-counting assays, strong bactericidal and fungicidal activity was observed, including bactericidal activity against food-borne pathogens Campylobacter jejuni, Salmonella enterica serovar Typhimurium, Clostridium perfringens, and Escherichia coli. Treatment with 16 μg/ml synthetic Gal-6 resulted in a 3 log unit reduction in Clostridium perfringens survival within 60 min, indicating fast killing kinetics. Transmission electron microscopy examination of synthetic-Gal-6-treated Clostridium perfringens cells showed dose-dependent changes in morphology after 30 min, including intracellular granulation, cytoplasm retraction, irregular septum formation in dividing cells, and cell lysis. The high expression in the proximal digestive tract and broad antimicrobial activity suggest that chicken β-defensin gallinacin-6 plays an important role in chicken innate host defense.

scholarly journals Antimicrobial activity of essential oil from Indonesian medicinal plants against food-borne pathogens

2018 ◽  
Vol 207 ◽  
pp. 012036 ◽  
Author(s):  
T Budiati ◽  
W Suryaningsih ◽  
S. Umaroh ◽  
B. Poerwanto ◽  
A Bakri ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Essential Oil ◽  
Food Borne Pathogens ◽  
Food Borne

Antimicrobial activity of thyme essential oil nanoemulsions on spoilage bacteria of fish and food-borne pathogens

2020 ◽  
Vol 36 ◽  
pp. 100635 ◽  
Author(s):  
Yesim Ozogul ◽  
Esmeray Kuley Boğa ◽  
Ismail Akyol ◽  
Mustafa Durmus ◽  
Yılmaz Ucar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Food Borne Pathogens ◽  
Thyme Essential Oil ◽  
Food Borne ◽  
Spoilage Bacteria

scholarly journals Antimicrobial activity of select edible plants from Odisha, India against food-borne pathogens

LWT ◽  
2019 ◽  
Vol 113 ◽  
pp. 108246 ◽  
Author(s):  
Sujogya Kumar Panda ◽  
Yugal Kishore Mohanta ◽  
Laxmipriya Padhi ◽  
Walter Luyten
Keyword(s):  
Antimicrobial Activity ◽  
Edible Plants ◽  
Food Borne Pathogens ◽  
Food Borne

In vitro antimicrobial activity of Muntingia calabura fruit extracts against food borne pathogens

2013 ◽  
Vol 5 (3) ◽  
pp. 135-136 ◽  
Author(s):  
G. Sibi ◽  
Kalpana Kaushik ◽  
Kalegowda Dhananjaya ◽  
Kodagahally R. Ravikumar ◽  
Honnaiah Mallesha
Keyword(s):  
Antimicrobial Activity ◽  
In Vitro Antimicrobial Activity ◽  
Fruit Extracts ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Muntingia Calabura

scholarly journals Escherichia coli and Salmonella spp. in meat in Jeddah, Saudi Arabia

2013 ◽  
Vol 7 (11) ◽  
pp. 812-818 ◽  
Author(s):  
Archana Iyer ◽  
Taha Kumosani ◽  
Soonham Yaghmoor ◽  
Elie Barbour ◽  
Esam Azhar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gastrointestinal Diseases ◽  
Developed Countries ◽  
Salmonella Spp ◽  
E Coli ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Microbiological Methods ◽  
Meat Samples ◽  
Butcher Shops

Introduction: Food-borne pathogens are the leading cause of illness and death in developing countries, killing approximately 1.8 million people annually. In developed countries, food-borne pathogens are responsible for millions of cases of infectious gastrointestinal diseases each year, costing billions of dollars. The objective of this study was to screen for two major food-borne pathogens, Escherichia coli and Salmonella spp., from meat samples obtained from different strata of the consumer market in Jeddah. Methodology: A total of 60 meat samples, 20 each from large hypermarkets, groceries and small butcher shops were used in the study. Samples were transported to the laboratory in a cooler. They were macerated in peptone water and then seeded on selective media appropriate for each organism. Colonies were identified using conventional microbiological methods and suspected colonies were confirmed as E. coli and Salmonella spp. by polymerase chain reaction (PCR) using specific primers. Results: The results indicated a high degree of contamination in samples from butcher shops as compared to those from groceries or hypermarkets (high scale supermarkets). Both pathogens E. coli and Salmonella spp. were found in higher rates in the samples from butcher shops. In small butcher shops, E. coli was found at an incidence of 65%, and Salmonella at 45%. Conclusion: The results indicate an urgent need for applying proper food hygienic practices in food outlets, especially in small ones, to reduce the incidence of food-borne diseases. Vigilance by the right agencies must be implemented in order to prevent future food-borne outbreaks.

Sign in / Sign up

Export Citation Format

Share Document