scholarly journals blaCTX-M-1/9/1Hybrid Genes May Have Been Generated fromblaCTX-M-15on an IncI2 Plasmid

2015 ◽  
Vol 59 (8) ◽  
pp. 4464-4470 ◽  
Author(s):  
Lanping Liu ◽  
Dandan He ◽  
Luchao Lv ◽  
Wuling Liu ◽  
Xiaojie Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Gene Sequences ◽  
Nucleotide Difference ◽  
Hybrid Gene ◽  
Content Type ◽  
Complete Sequencing ◽  
Sequence Types ◽  
M 14 ◽  
Plasmid Backbone

ABSTRACTThree hybrid CTX-M β-lactamases, CTX-M-64, CTX-M-123, and CTX-M-132, with N and C termini matching CTX-M-1 group enzymes and centers matching CTX-M-9 group enzymes, have been identified. The hybrid gene sequences suggested recombination betweenblaCTX-M-15andblaCTX-M-14, the two most commonblaCTX-Mvariants worldwide. However,blaCTX-M-64andblaCTX-M-123are found in an ISEcp1-blaCTX-Mtransposition unit with a 45-bp “spacer,” rather than the 48 bp usually associated withblaCTX-M-15, and 112 bp of IncA/C plasmid backbone. This is closer to the context ofblaCTX-M-55, which has one nucleotide difference fromblaCTX-M-15, on IncI2 plasmid pHN1122-1. Here, we characterized an IncI2 plasmid carryingblaCTX-M-15with a 45-bp spacer (pHNY2-1) by complete sequencing and also sequenced IncI2 plasmids carryingblaCTX-M-64(pHNAH46-1) orblaCTX-M-132(pHNLDH19) and an IncI1 plasmid carryingblaCTX-M-123(pHNAH4-1). pHNY2-1 has the same ISEcp1-blaCTX-M-IncA/C insertion as pHN1122-1, pHNAH46-1, and pHNLDH19, and all four plasmid backbones are almost identical. pHNAH4-1 (IncI1 sequence type 108 [ST108]) carries a transposition unit that includes a 2,720-bp fragment of the IncI2 backbone, suggesting ISEcp1-mediated transfer ofblaCTX-M-IncA/C-IncI2 to an IncI1 plasmid. All three hybridblaCTX-Mgenes may have resulted from recombination betweenblaCTX-M-14andblaCTX-M-15with a 45-bp spacer on an IncI2 plasmid. Five additionalEscherichia coliisolates of different sequence types from different provinces, farms, and/or animals hadblaCTX-M-64on a pHNAH46-1-like IncI2 plasmid and 9 hadblaCTX-M-123on a pHNAH4-1-like IncI1 ST108 plasmid. Thus, epidemic IncI plasmids may be responsible for the spread ofblaCTX-M-64andblaCTX-M-123between different animals and different locations in China.

scholarly journals Travelers Can Import Colistin-Resistant Enterobacteriaceae, Including Those Possessing the Plasmid-Mediatedmcr-1Gene

2016 ◽  
Vol 60 (8) ◽  
pp. 5080-5084 ◽  
Author(s):  
Odette J. Bernasconi ◽  
Esther Kuenzli ◽  
João Pires ◽  
Regula Tinguely ◽  
Alessandra Carattoli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Extended Spectrum ◽  
Stool Samples ◽  
Plasmid Backbone

ABSTRACTStool samples from 38 travelers returning from India were screened for extended-spectrum cephalosporin- and carbapenem-resistantEnterobacteriaceaeimplementing standard selective plates. Twenty-six (76.3%) people were colonized with CTX-M or DHA producers, but none of the strains was colistin resistant and/ormcr-1positive. Nevertheless, using overnight enrichment and CHROMagar Orientation plates supplemented with colistin, four people (10.5%) were found to be colonized with colistin-resistantEscherichia coli. One cephalosporin-susceptible sequence type 10 (ST10) strain carried a 4,211-bp ISApl1-mcr-1-ISApl1element in an IncHI2 plasmid backbone.

scholarly journals Complete Sequencing of Plasmids ContainingblaOXA-163andblaOXA-48in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (11) ◽  
pp. 6948-6951 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Sequence Type ◽  
Content Type ◽  
Extended Spectrum ◽  
Complete Sequencing

ABSTRACTOXA-48-like enzymes have emerged as important extended-spectrum β-lactamases/carbapenemases inEscherichia colisequence type 131 (ST131). We report the structures of the first fully sequencedblaOXA-163plasmid and of two otherblaOXA-48plasmids in this lineage.blaOXA-163was located on a 71-kb IncN plasmid with other resistance genes.blaOXA-48was present on IncL/M plasmids, genetically similar to otherblaOXA-48plasmid sequences, and consistent with interspecies/interlineage spread. The presence ofblaOXA-48-likegenes on epidemic plasmids in ST131 is of concern.

scholarly journals New Small Plasmid HarboringblaKPC-2in Pseudomonas aeruginosa

2016 ◽  
Vol 60 (5) ◽  
pp. 3211-3214 ◽  
Author(s):  
Renata Galetti ◽  
Leonardo Neves Andrade ◽  
Michael Chandler ◽  
Alessandro de Mello Varani ◽  
Ana Lúcia Costa Darini
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Inverted Repeat ◽  
Sequence Type ◽  
Small Plasmid ◽  
Content Type ◽  
Complete Sequencing ◽  
Genetic Context

ABSTRACTThe aim of this study was to characterize the genetic context ofblaKPC-2inPseudomonas aeruginosasequence type 244 from Brazil. TheblaKPC-2gene was detected in a new small plasmid, pBH6. Complete sequencing revealed that pBH6 was 3,652 bp long and included the Tn3resolvase and Tn3inverted repeat (IR), a partial copy of ISKpn6, and a putativeoriregion but norepgenes. pBH6 replicated stably intoEscherichia colistrain DH10B andP. aeruginosastrain PAO.

scholarly journals High-Resolution Melting Analysis for Rapid Detection of Sequence Type 131 Escherichia coli

2017 ◽  
Vol 61 (6) ◽  
Author(s):  
Lucas B. Harrison ◽  
Nancy D. Hanson
Keyword(s):  
Escherichia Coli ◽  
Principal Component Analysis ◽  
High Resolution ◽  
High Resolution Melting ◽  
Principal Component ◽  
Cost Effective ◽  
Sequence Type ◽  
Content Type ◽  
E Coli ◽  
Sequence Types

ABSTRACT Escherichia coli isolates belonging to the sequence type 131 (ST131) clonal complex have been associated with the global distribution of fluoroquinolone and β-lactam resistance. Whole-genome sequencing and multilocus sequence typing identify sequence type but are expensive when evaluating large numbers of samples. This study was designed to develop a cost-effective screening tool using high-resolution melting (HRM) analysis to differentiate ST131 from non-ST131 E. coli in large sample populations in the absence of sequence analysis. The method was optimized using DNA from 12 E. coli isolates. Singleplex PCR was performed using 10 ng of DNA, Type-it HRM buffer, and multilocus sequence typing primers and was followed by multiplex PCR. The amplicon sizes ranged from 630 to 737 bp. Melt temperature peaks were determined by performing HRM analysis at 0.1°C resolution from 50 to 95°C on a Rotor-Gene Q 5-plex HRM system. Derivative melt curves were compared between sequence types and analyzed by principal component analysis. A blinded study of 191 E. coli isolates of ST131 and unknown sequence types validated this methodology. This methodology returned 99.2% specificity (124 true negatives and 1 false positive) and 100% sensitivity (66 true positives and 0 false negatives). This HRM methodology distinguishes ST131 from non-ST131 E. coli without sequence analysis. The analysis can be accomplished in about 3 h in any laboratory with an HRM-capable instrument and principal component analysis software. Therefore, this assay is a fast and cost-effective alternative to sequencing-based ST131 identification.

scholarly journals blaNDM-5Carried by an IncX3 Plasmid in Escherichia coli Sequence Type 167

2014 ◽  
Vol 58 (12) ◽  
pp. 7548-7552 ◽  
Author(s):  
Ping Yang ◽  
Yi Xie ◽  
Ping Feng ◽  
Zhiyong Zong
Keyword(s):  
Escherichia Coli ◽  
Chinese Patient ◽  
Sequence Type ◽  
Genomic Sequencing ◽  
Travel History ◽  
Content Type ◽  
Resistance Determinants ◽  
Genetic Context ◽  
M 14

ABSTRACTblaNDM-5was found inEscherichia colistrain 0215 from a Chinese patient without travel history. Genomic sequencing and conjugation experiments were performed. Strain 0215 belonged to sequence type 167 (ST167) and had other resistance determinants, includingblaTEM-135,blaCTX-M-14, andaac(6′)-Ib. blaNDM-5was carried by a 47-kb self-transmissible IncX3 plasmid and was in a complex genetic context similar to that ofblaNDM-1on IncX3 plasmids. IncX3 plasmids might have emerged as a common vehicle mediating the spread ofblaNDM.

scholarly journals Widespread Dissemination of CTX-M-15 Genotype Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae among Patients Presenting to Community Hospitals in the Southeastern United States

2013 ◽  
Vol 58 (2) ◽  
pp. 1200-1202 ◽  
Author(s):  
Luke F. Chen ◽  
Joshua T. Freeman ◽  
Brad Nicholson ◽  
Anna Keiger ◽  
Sarah Lancaster ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
North Carolina ◽  
Southeastern United States ◽  
Sequence Type ◽  
Community Hospitals ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Widespread Dissemination ◽  
M 14

ABSTRACTExtended-spectrum-β-lactamase (ESBL)-producing organisms are increasingly prevalent. We determined the characteristics of 66 consecutive ESBL-producing isolates from six community hospitals in North Carolina and Virginia from 2010 to 2012. Fifty-three (80%) ESBL-producing isolates contained CTX-M enzymes; CTX-M-15 was found in 68% ofEscherichia coliand 73% ofKlebsiellaisolates. Sequence type 131 (ST131) was the commonest type ofE. coli, accounting for 48% of CTX-M-15-producing and 66% of CTX-M-14-producing isolates. In conclusion, the CTX-M genotype and ST131E. coliwere common among ESBL isolates from U.S. community hospitals.

scholarly journals Various Sequence Types of Enterobacteriaceae Isolated from Commercial Chicken Farms in China and Carrying the blaNDM-5 Gene

2018 ◽  
Vol 62 (10) ◽  
Author(s):  
Rong Xiang ◽  
An-Yun Zhang ◽  
Xiao-Lan Ye ◽  
Zhuang-Zhuang Kang ◽  
Chang-Wei Lei ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Sequence Type ◽  
Content Type ◽  
Commercial Chicken ◽  
Rectal Swabs ◽  
Sequence Types

ABSTRACT A total of 108 meropenem-resistant Enterobacteriaceae isolates were obtained from 1,658 rectal swabs collected from 15 unrelated commercial chicken farms in China between 2014 and 2016. These samples yielded 16 Escherichia coli and 2 Klebsiella pneumoniae isolates of diverse sequence types carrying a blaNDM-5-bearing IncX3 plasmid. K. pneumoniae strain sequence type 709 (ST709) has two blaNDM-5-carrying plasmids that were transferred together to E.coli.

scholarly journals Genome Sequences of Enteroinvasive Escherichia coli Sequence Type 6, 99, and 311 Strains Acquired in Asia Pacific

2019 ◽  
Vol 8 (36) ◽  
Author(s):  
Rajat Dhakal ◽  
Qinning Wang ◽  
Peter Howard ◽  
Vitali Sintchenko
Keyword(s):  
Escherichia Coli ◽  
Draft Genome ◽  
Laboratory Diagnosis ◽  
Research Community ◽  
Sequence Type ◽  
Asia Pacific ◽  
Genome Sequences ◽  
Content Type ◽  
Distinct Sequence ◽  
Sequence Types

Laboratory diagnosis of enteroinvasive Escherichia coli (EIEC) remains difficult and limits the availability of EIEC genomes to the research community. We report the draft genome sequences of three EIEC strains which represent three distinct sequence types and serotypes circulating in Asia Pacific and causing enterocolitis in humans.

scholarly journals Molecular Features of Community-Associated Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strains in the United States

2014 ◽  
Vol 58 (11) ◽  
pp. 6953-6957 ◽  
Author(s):  
Fupin Hu ◽  
Jessica A. O'Hara ◽  
Jesabel I. Rivera ◽  
Yohei Doi
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Restriction Analysis ◽  
The United States ◽  
Sequence Type ◽  
Content Type ◽  
Molecular Features ◽  
Extended Spectrum ◽  
Sequence Types ◽  
Replicon Type

ABSTRACTWe characterized 30 community-associated extended-spectrum-β-lactamase-producingEscherichia coliisolates collected from five hospitals in the United States. Nineteen sequence types were identified. All sequence type 131 (ST131) isolates had thefimH30 allele. IncFII-FIA-FIB was the most common replicon type among theblaCTX-M-carrying plasmids, followed by IncFII-FIA and IncA/C. Restriction analysis of the IncFII-FIA-FIB and IncFII-FIA plasmids yielded related profiles for plasmids originating from different hospitals. The plasmids containingblaCTX-MorblaSHVwere stably maintained after serial passages.

scholarly journals A Plasmid Bearing theblaCTX-M-15Gene and Phage P1-Like Sequences from a Sequence Type 11 Klebsiella pneumoniae Isolate

2015 ◽  
Vol 59 (10) ◽  
pp. 6608-6610 ◽  
Author(s):  
Juyoun Shin ◽  
Kwan Soo Ko
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
South Korea ◽  
Resistance Genes ◽  
Sequence Type ◽  
Content Type ◽  
Plasmid Backbone

ABSTRACTPlasmid pKP12226 was extracted and analyzed from a CTX-M-15-producingKlebsiella pneumoniaesequence type 11 (ST11) isolate collected in South Korea. The plasmid represents chimeric characteristics consisting of a pIP1206-like backbone and lysogenized phage P1-like sequences. It bears a resistance region that includes resistance genes to several antibiotics and is different from previously characterized plasmids from South Korea bearingblaCTX-M-15. It may have resulted from recombination between anEscherichia coliplasmid backbone, ablaCTX-M-15-bearing resistance region, and lysogenized phage P1-like sequences.

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