scholarly journals Complete Sequencing of Plasmids ContainingblaOXA-163andblaOXA-48in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (11) ◽  
pp. 6948-6951 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Resistance Genes ◽  
Sequence Type ◽  
Content Type ◽  
Extended Spectrum ◽  
Complete Sequencing

ABSTRACTOXA-48-like enzymes have emerged as important extended-spectrum β-lactamases/carbapenemases inEscherichia colisequence type 131 (ST131). We report the structures of the first fully sequencedblaOXA-163plasmid and of two otherblaOXA-48plasmids in this lineage.blaOXA-163was located on a 71-kb IncN plasmid with other resistance genes.blaOXA-48was present on IncL/M plasmids, genetically similar to otherblaOXA-48plasmid sequences, and consistent with interspecies/interlineage spread. The presence ofblaOXA-48-likegenes on epidemic plasmids in ST131 is of concern.

scholarly journals Characterization of IncI1 Sequence Type 71 Epidemic Plasmid Lineage Responsible for the Recent Dissemination of CTX-M-65 Extended-Spectrum β-Lactamase in the Bolivian Chaco Region

2015 ◽  
Vol 59 (9) ◽  
pp. 5340-5347 ◽  
Author(s):  
Eleonora Riccobono ◽  
Vincenzo Di Pilato ◽  
Tiziana Di Maggio ◽  
Carmen Revollo ◽  
Alessandro Bartoloni ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Oxytoca ◽  
Sequence Type ◽  
Healthy Children ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Chaco Region ◽  
Complete Sequencing ◽  
Biological Cost

ABSTRACTDuring the last decade, a significant diffusion of CTX-M-type extended-spectrum β-lactamases (ESBLs) was observed in commensalEscherichia colifrom healthy children in the Bolivian Chaco region, with initial dissemination of CTX-M-2, which was then replaced by CTX-M-15 and CTX-M-65. In this work, we demonstrate that the widespread dissemination of CTX-M-65 observed in this context was related to the polyclonal spreading of an IncI1 sequence type 71 (ST71) epidemic plasmid lineage. The structure of the epidemic plasmid population was characterized by complete sequencing of four representatives and PCR mapping of the remainder (n= 16). Sequence analysis showed identical plasmid backbones (similar to that of the reference IncI1 plasmid, R64) and a multiresistance region (MRR), which underwent local microevolution. The MRR harbored genes responsible for resistance to β-lactams, aminoglycosides, florfenicol, and fosfomycin (with microevolution mainly consisting of deletion events of resistance modules). TheblaCTX-M-65module harbored by the IncI1 ST71 epidemic plasmid was apparently derived from IncN-type plasmids, likely via IS26-mediated mobilization. The plasmid could be transferred by conjugation to several different enterobacterial species (Escherichia coli,Cronobacter sakazakii,Enterobacter cloacae,Klebsiella oxytoca,Klebsiella pneumoniae, andSalmonella enterica) and was stably maintained without selective pressure in these species, with the exception ofK. oxytocaandS. enterica. Fitness assays performed inE. colirecipients demonstrated that the presence of the epidemic plasmid was apparently not associated with a significant biological cost.

Prevalence and molecular epidemiology of plasmid-mediated fosfomycin resistance genes among blood and urinary Escherichia coli isolates

2013 ◽  
Vol 62 (11) ◽  
pp. 1707-1713 ◽  
Author(s):  
Pak-Leung Ho ◽  
Jane Chan ◽  
Wai-U Lo ◽  
Eileen L. Lai ◽  
Yuk-Yam Cheung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Blood Culture ◽  
Molecular Epidemiology ◽  
Resistance Genes ◽  
Rflp Analysis ◽  
Sequence Type ◽  
E Coli ◽  
Complete Sequencing ◽  
Fosfomycin Resistance ◽  
Urinary Isolates

A total of 1878 non-duplicate clinical Escherichia coli isolates (comprising 1711 urinary isolates and 167 blood-culture isolates), which were collected from multiple centres in Hong Kong during 1996–2008, were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin (fos) resistance genes. Eighteen of the 1878 clinical E. coli isolates were fosfomycin resistant, of which six were fosA3 positive and two were positive for another fosA variant (designated fosKP96). No isolates had the fosC2 gene. The clones of the eight isolates were diverse: sequence type (ST) 95 (n = 2), ST118 (n = 1), ST131 (n = 1), ST617 (n = 1), ST648 (n = 1), ST1488 (n = 1) and ST2847 (n = 1). In the isolates, fosA3 and bla CTX-M genes were co-harboured on conjugative plasmids with F2:A−:B− (n = 2), N (n = 1), F–:A−:B1 and N (n = 1) and untypable (n = 2) replicons. Both fosKP96-carrying plasmids belonged to replicon N. RFLP analysis showed that the two F2:A−:B− plasmids carrying fosA3 and bla CTX-M-3 genes shared the same pattern. Complete sequencing of one of the two F2:A−:B− plasmids, pFOS-HK151325 (69 768 bp) demonstrated it to be >99 % identical to the previously sequenced plasmid pHK23a originating from a pig E. coli isolate in the same region. This study demonstrated the dissemination of fosA3 genes in diverse E. coli clones on multiple bla CTX-M-carrying plasmid types, of which F2:A−:B− plasmids closely related to pHK23a were shared by isolates from human and animal sources.

scholarly journals Spread of NDM-5 and OXA-181 Carbapenemase-Producing Escherichia coli in Chad

2019 ◽  
Vol 63 (11) ◽  
Author(s):  
Oumar Ouchar Mahamat ◽  
Manon Lounnas ◽  
Mallorie Hide ◽  
Abelsalam Tidjani ◽  
Julio Benavides ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Healthy Volunteers ◽  
Resistance Genes ◽  
Sequence Type ◽  
Hospitalized Patients ◽  
Content Type ◽  
E Coli ◽  
First Time

ABSTRACT We detected for the first time blaNDM-5 and blaOXA-181 in Escherichia coli isolates from hospitalized patients and healthy volunteers in Chad. These resistance genes were located on IncX3 and IncF plasmids. Despite the large diversity of E. coli clones, the identified resistant intestinal isolates belonged mainly to the same sequence type.

scholarly journals Occurrence of blaKPC-2, blaCTX-M, and mcr-1 in Enterobacteriaceae from Well Water in Rural China

2017 ◽  
Vol 61 (4) ◽  
Author(s):  
Pan Sun ◽  
Zhenwang Bi ◽  
Maud Nilsson ◽  
Beiwen Zheng ◽  
Björn Berglund ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Rural China ◽  
Multidrug Resistant ◽  
Sequence Type ◽  
Well Water ◽  
Content Type ◽  
Potential Source ◽  
Extended Spectrum

ABSTRACT We report on the coexistence of mcr-1 and bla CTX-M in multidrug-resistant, extended-spectrum β-lactamase-producing Escherichia coli belonging to the sequence type 10 complex isolated from well water in rural China. Raoultella ornithinolytica with bla KPC-2 was also detected in well water from the same area. This study shows that genes coding for resistance to last-resort antibiotics are present in wells in rural China, indicating a potential source of antibiotic resistance.

scholarly journals Travelers Can Import Colistin-Resistant Enterobacteriaceae, Including Those Possessing the Plasmid-Mediatedmcr-1Gene

2016 ◽  
Vol 60 (8) ◽  
pp. 5080-5084 ◽  
Author(s):  
Odette J. Bernasconi ◽  
Esther Kuenzli ◽  
João Pires ◽  
Regula Tinguely ◽  
Alessandra Carattoli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Type ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Extended Spectrum ◽  
Stool Samples ◽  
Plasmid Backbone

ABSTRACTStool samples from 38 travelers returning from India were screened for extended-spectrum cephalosporin- and carbapenem-resistantEnterobacteriaceaeimplementing standard selective plates. Twenty-six (76.3%) people were colonized with CTX-M or DHA producers, but none of the strains was colistin resistant and/ormcr-1positive. Nevertheless, using overnight enrichment and CHROMagar Orientation plates supplemented with colistin, four people (10.5%) were found to be colonized with colistin-resistantEscherichia coli. One cephalosporin-susceptible sequence type 10 (ST10) strain carried a 4,211-bp ISApl1-mcr-1-ISApl1element in an IncHI2 plasmid backbone.

scholarly journals ESKAPE Bacteria and Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolated from Wastewater and Process Water from German Poultry Slaughterhouses

2020 ◽  
Vol 86 (8) ◽  
Author(s):  
Mykhailo Savin ◽  
Gabriele Bierbaum ◽  
Jens Andre Hammerl ◽  
Céline Heinemann ◽  
Marijo Parcina ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Wastewater Treatment ◽  
Resistance Genes ◽  
Wastewater Treatment Plants ◽  
Process Water ◽  
Resistant Bacteria ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Clinically Relevant Bacteria

ABSTRACT The wastewater of livestock slaughterhouses is considered a source of antimicrobial-resistant bacteria with clinical relevance and may thus be important for their dissemination into the environment. To get an overview of their occurrence and characteristics, we investigated process water (n = 50) from delivery and unclean areas as well as wastewater (n = 32) from the in-house wastewater treatment plants (WWTPs) of two German poultry slaughterhouses (slaughterhouses S1 and S2). The samples were screened for ESKAPE bacteria (Enterococcus spp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp.) and Escherichia coli. Their antimicrobial resistance phenotypes and the presence of extended-spectrum-β-lactamase (ESBL), carbapenemase, and mobilizable colistin resistance genes were determined. Selected ESKAPE bacteria were epidemiologically classified using different molecular typing techniques. At least one of the target species was detected in 87.5% (n = 28/32) of the wastewater samples and 86.0% (n = 43/50) of the process water samples. The vast majority of the recovered isolates (94.9%, n = 448/472) was represented by E. coli (39.4%), the A. calcoaceticus-A. baumannii (ACB) complex (32.4%), S. aureus (12.3%), and K. pneumoniae (10.8%), which were widely distributed in the delivery and unclean areas of the individual slaughterhouses, including their wastewater effluents. Enterobacter spp., Enterococcus spp., and P. aeruginosa were less abundant and made up 5.1% of the isolates. Phenotypic and genotypic analyses revealed that the recovered isolates exhibited diverse resistance phenotypes and β-lactamase genes. In conclusion, wastewater effluents from the investigated poultry slaughterhouses exhibited clinically relevant bacteria (E. coli, methicillin-resistant S. aureus, K. pneumoniae, and species of the ACB and Enterobacter cloacae complexes) that contribute to the dissemination of clinically relevant resistances (i.e., blaCTX-M or blaSHV and mcr-1) in the environment. IMPORTANCE Bacteria from livestock may be opportunistic pathogens and carriers of clinically relevant resistance genes, as many antimicrobials are used in both veterinary and human medicine. They may be released into the environment from wastewater treatment plants (WWTPs), which are influenced by wastewater from slaughterhouses, thereby endangering public health. Moreover, process water that accumulates during the slaughtering of poultry is an important reservoir for livestock-associated multidrug-resistant bacteria and may serve as a vector of transmission to occupationally exposed slaughterhouse employees. Mitigation solutions aimed at the reduction of the bacterial discharge into the production water circuit as well as interventions against their further transmission and dissemination need to be elaborated. Furthermore, the efficacy of in-house WWTPs needs to be questioned. Reliable data on the occurrence and diversity of clinically relevant bacteria within the slaughtering production chain and in the WWTP effluents in Germany will help to assess their impact on public and environmental health.

scholarly journals New Small Plasmid HarboringblaKPC-2in Pseudomonas aeruginosa

2016 ◽  
Vol 60 (5) ◽  
pp. 3211-3214 ◽  
Author(s):  
Renata Galetti ◽  
Leonardo Neves Andrade ◽  
Michael Chandler ◽  
Alessandro de Mello Varani ◽  
Ana Lúcia Costa Darini
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Inverted Repeat ◽  
Sequence Type ◽  
Small Plasmid ◽  
Content Type ◽  
Complete Sequencing ◽  
Genetic Context

ABSTRACTThe aim of this study was to characterize the genetic context ofblaKPC-2inPseudomonas aeruginosasequence type 244 from Brazil. TheblaKPC-2gene was detected in a new small plasmid, pBH6. Complete sequencing revealed that pBH6 was 3,652 bp long and included the Tn3resolvase and Tn3inverted repeat (IR), a partial copy of ISKpn6, and a putativeoriregion but norepgenes. pBH6 replicated stably intoEscherichia colistrain DH10B andP. aeruginosastrain PAO.

scholarly journals War Wound Treatment Complications Due to Transfer of an IncN Plasmid HarboringblaOXA-181from Morganella morganii to CTX-M-27-Producing Sequence Type 131 Escherichia coli

2015 ◽  
Vol 59 (6) ◽  
pp. 3556-3562 ◽  
Author(s):  
Patrick McGann ◽  
Erik Snesrud ◽  
Ana C. Ong ◽  
Lakshmi Appalla ◽  
Michael Koren ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Molecular Techniques ◽  
Morganella Morganii ◽  
Genetic Changes ◽  
Content Type ◽  
Surgical Interventions ◽  
E Coli ◽  
Extended Spectrum

ABSTRACTA 22-year-old male developed a recurrent sacral abscess associated with embedded shrapnel following a blast injury. Cultures grew extended-spectrum β-lactamase (ESBL)-producing, carbapenem-susceptibleEscherichia coli. Ertapenem was administered, but the infection recurred after each course of antibiotics. Initial surgical interventions were unsuccessful, and subsequent cultures yieldedE. coliandMorganella morganii, both nonsusceptible to carbapenems. The isolates were Carba NP test negative, gave ambiguous results with the modified Hodge test, and amplified theblaOXA48-like gene by real-time PCR. AllE. coliisolates were sequence type 131 (ST131), carried nine resistance genes (includingblaCTX-M-27) on an IncF plasmid, and were identical by genome sequencing, except for 150 kb of plasmid DNA in carbapenem-nonsusceptible isolates only. Sixty kilobases of this was shared byM. morganiiand represented an IncN plasmid harboringblaOXA-181. InM. morganii, the gene was flanked by IS3000and ISKpn19, but in all but one of theE. coliisolates containingblaOXA-181, a second copy of ISKpn19had inserted adjacent to IS3000. To the best of our knowledge, this is the first report ofblaOXA-181in the virulent ST131 clonal group and carried by the promiscuous IncN family of plasmids. The tendency ofM. morganiito have high MICs of imipenem, ablaOXA-181substrate profile that includes penicillins but not extended-spectrum cephalosporins, and weak carbapenemase activity almost resulted in the presence ofblaOXA-181being overlooked. We highlight the importance of surveillance for carbapenem resistance in all species, even those with intrinsic resistances, and the value of advanced molecular techniques in detecting subtle genetic changes.

scholarly journals Multiresistant Uropathogenic Escherichia coli from a Region in India Where Urinary Tract Infections Are Endemic: Genotypic and Phenotypic Characteristics of Sequence Type 131 Isolates of the CTX-M-15 Extended-Spectrum-β-Lactamase-Producing Lineage

2012 ◽  
Vol 56 (12) ◽  
pp. 6358-6365 ◽  
Author(s):  
Arif Hussain ◽  
Christa Ewers ◽  
Nishant Nandanwar ◽  
Sebastian Guenther ◽  
Savita Jadhav ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Sequence Type ◽  
Health Concern ◽  
Beta Lactam ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Tract Infections

ABSTRACTEscherichia colisequence type 131 (O25b:H4), associated with the CTX-M-15 extended-spectrum beta-lactamases (ESBLs) and linked predominantly to the community-onset antimicrobial-resistant infections, has globally emerged as a public health concern. However, scant attention is given to the understanding of the molecular epidemiology of these strains in high-burden countries such as India. Of the 100 clinicalE. coliisolates obtained by us from a setting where urinary tract infections are endemic, 16 ST131E. coliisolates were identified by multilocus sequence typing (MLST). Further, genotyping and phenotyping methods were employed to characterize their virulence and drug resistance patterns. All the 16 ST131 isolates harbored the CTX-M-15 gene, and half of them also carried TEM-1; 11 of these were positive forblaOXAgroups 1 and 12 foraac(6′)-Ib-cr. At least 12 isolates were refractory to four non-beta-lactam antibiotics: ciprofloxacin, gentamicin, sulfamethoxazole-trimethoprim, and tetracycline. Nine isolates carried the class 1 integron. Plasmid analysis indicated a large pool of up to six plasmids per strain with a mean of approximately three plasmids. Conjugation and PCR-based replicon typing (PBRT) revealed that the spread of resistance was associated with the FIA incompatibility group of plasmids. Pulsed-field gel electrophoresis (PFGE) and genotyping of the virulence genes showed a low level of diversity among these strains. The association of ESBL-encoding plasmid with virulence was demonstrated in transconjugants by serum assay. None of the 16 ST131 ESBL-producingE. colistrains were known to synthesize carbapenemase enzymes. In conclusion, our study reports a snapshot of the highly virulent/multiresistant clone ST131 of uropathogenicE. colifrom India. This study suggests that the ST131 genotypes from this region are clonally evolved and are strongly associated with the CTX-M-15 enzyme, carry a high antibiotic resistance background, and have emerged as an important cause of community-acquired urinary tract infections.

scholarly journals Characteristics of CTX-M Extended-Spectrum β-Lactamase-Producing Escherichia coli Strains Isolated from Multiple Rivers in Southern Taiwan

2016 ◽  
Vol 82 (6) ◽  
pp. 1889-1897 ◽  
Author(s):  
Po-An Chen ◽  
Chih-Hsin Hung ◽  
Ping-Chih Huang ◽  
Jung-Ren Chen ◽  
I-Fei Huang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
River Water ◽  
Sequence Type ◽  
Content Type ◽  
Mlst Analysis ◽  
River Waters ◽  
E Coli ◽  
Extended Spectrum ◽  
Southern Taiwan ◽  
Tract Infections

ABSTRACTExtended-spectrum β-lactamase (ESBL)-producingEscherichia colisequence type ST131 has emerged as the leading cause of community-acquired urinary tract infections and bacteremia worldwide. Whether environmental water is a potential reservoir of these strains remains unclear. River water samples were collected from 40 stations in southern Taiwan from February to August 2014. PCR assay and multilocus sequence typing (MLST) analysis were conducted to determine the CTX-M group and sequence type, respectively. In addition, we identified the seasonal frequency of ESBL-producingE. colistrains and their geographical relationship with runoffs from livestock and poultry farms between February and August 2014. ESBL-producingE. coliaccounted for 30% of the 621E. colistrains isolated from river water in southern Taiwan. ESBL-producingE. coliST131 was not detected among the isolates. The most commonly detected strain wasE. coliCTX-M group 9. Among the 92 isolates selected for MLST analysis, the most common ESBL-producing clonal complexes were ST10 and ST58. The proportion of ESBL-producingE. coliwas significantly higher in areas with a lower river pollution index (P= 0.025) and regions with a large number of chickens being raised (P= 0.013). ESBL-producingE. colistrains were commonly isolated from river waters in southern Taiwan. The most commonly isolated ESBL-producing clonal complexes were ST10 and ST58, which were geographically related to chicken farms. ESBL-producingE. coliST131, the major clone causing community-acquired infections in Taiwan and worldwide, was not detected in river waters.

Sign in / Sign up

Export Citation Format

Share Document