A nutrient medium for development of cell dense inoculum in mixotrophic mode to seed mass culture units of Dunaliella salina

2015 ◽  
Vol 147 (1) ◽  
pp. 7-28
Author(s):  
Suman Keerthi ◽  
Uma Devi Koduru ◽  
Nittala S. Sarma
Keyword(s):  
Mass Culture ◽  
Nutrient Medium ◽  
Dunaliella Salina ◽  
Seed Mass

The mass culture of Dunaliella salina for fine chemicals: From laboratory to pilot plant

Hydrobiologia ◽  
1984 ◽  
Vol 116-117 (1) ◽  
pp. 115-121 ◽  
Author(s):  
L. J. Borowitzka ◽  
M. A. Borowitzka ◽  
T. P. Moulton
Keyword(s):  
Mass Culture ◽  
Pilot Plant ◽  
Dunaliella Salina ◽  
Fine Chemicals

scholarly journals Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content

Marine Drugs ◽  
2021 ◽  
Vol 19 (7) ◽  
pp. 381
Author(s):  
Anna Andreeva ◽  
Ekaterina Budenkova ◽  
Olga Babich ◽  
Stanislav Sukhikh ◽  
Vyacheslav Dolganyuk ◽  
...  
Keyword(s):  
Growth Rate ◽  
Chlorella Vulgaris ◽  
Nutrient Medium ◽  
Dunaliella Salina ◽  
Arthrospira Platensis ◽  
Carbohydrate Content ◽  
Biofuel Production ◽  
Total Carbohydrate ◽  
Microalgae Biomass ◽  
Total Carbohydrates

Our study focused on investigating the possibilities of controlling the accumulation of carbohydrates in certain microalgae species (Arthrospira platensis Gomont, Chlorella vulgaris Beijer, and Dunaliella salina Teod) to determine their potential in biofuel production (biohydrogen). It was found that after the introduction of carbohydrates (0.05 g⋅L−1) into the nutrient medium, the growth rate of the microalgae biomass increased, and the accumulation of carbohydrates reached 41.1%, 47.9%, and 31.7% for Arthrospira platensis, Chlorella vulgaris, and Dunaliella salina, respectively. Chlorella vulgaris had the highest total carbohydrate content (a mixture of glucose, fructose, sucrose, and maltose, 16.97%) among the studied microalgae, while for Arthrospira platensis and Dunaliella salina, the accumulation of total carbohydrates was 9.59% and 8.68%, respectively. Thus, the introduction of carbohydrates into the nutrient medium can stimulate their accumulation in the microalgae biomass, an application of biofuel production (biohydrogen).

The mass culture of Dunaliella salina for ?-carotene: from pilot plant to production plant

Hydrobiologia ◽  
1987 ◽  
Vol 151-152 (1) ◽  
pp. 99-105 ◽  
Author(s):  
T. P. Moulton ◽  
L. J. Borowitzka ◽  
D. J. Vincent
Keyword(s):  
Mass Culture ◽  
Pilot Plant ◽  
Dunaliella Salina ◽  
Production Plant

scholarly journals POTENSI PRODUKSI BIOFUEL DARI BIOMASSA FITOPLANKTON LAUT SPESIES Chlorella vulgaris, Dunaliella salina DAN Spirulina sp., YANG DITUMBUHKAN DALAM NUTRIEN UNGGUL “MSSIP” TERINDUKSI ION LOGAM Fe, Co, DAN Ni

KOVALEN ◽  
2017 ◽  
Vol 3 (1) ◽  
pp. 89
Author(s):  
Syahruddin Kasim ◽  
Paulina Taba ◽  
Indah Raya ◽  
Ruslan Ruslan
Keyword(s):  
Metal Ions ◽  
Chlorella Vulgaris ◽  
Mass Culture ◽  
Phytoplankton Biomass ◽  
Dunaliella Salina ◽  
Growth Parameters ◽  
Raw Materials ◽  
Light Exposure ◽  
Initial Density

Research about composition of superior nutrients, growth parameters, and best method to maximize production of biomass sea phytoplankton, Chlorella  vulgaris, Dunaliella salina, and Spirulina sp. has been performed. The nutrients was named as MSSIP which were consist of following compositions: urea fertilizer, Arschat-M nutrient, Fe:Co:Ni metal ions (6 : 3 : 9 : 6 : 3). Methods used were identification and analysis of sea phytoplankton.  Determination of optimum growth condition, pure culture, and mass culture were using local raw materials-based engineered nutrients or MSSIP (M. Sjahrul-Syahruddin Kasim-Indah Raya-Paulina Taba). Determination of product density of sea phytoplankton biomass, analyses of carbohydrate content, and lipid biomass were done by using haemocytometer and microscope, Luff Schrol method, and soxhlet method (n-hexane as solvent), respectively. Morphology of phytoplankton was identified by using a digital camera microscope, SZ60/sZ60-61. Furthermore, to understand the effect of metal ions, Fe, Co, and Ni added into MSSIP nutrient, identification of nutrient before and after culturing process were done using XRF-Thermo-Fisher.  The results showed that Chlorella  vulgaris, Dunaliella salina, and Spirulina sp. were suitable as raw materials  to produce biofuel. Those three phytoplankton contained 0,3095 g/L, 0,3782 g/L, and 0,3325 g/L biomass, 32,49%w/w, 31,58%w/w, and 29,81%w/w carbohydrates; and  25,95%w/w, 26,82%w/w and 24,53%w/w lipid, respectively. Best optimum condition of culture were salinity of 30-35%, temperature of 20-30 °C, pH of 8-9, initial density of 2.5 x 104 cell/mL, light exposure of 40 watt, and continuously aerated with CO2. Based on our study, sea phytoplankton, Chlorella  vulgaris, Dunaliella salina, and Spirulina sp. have a high potency as source of bioethanol and biodiesel.Keywords: mass culture, superior nutrient MSSIP, sea phytoplankton biomass, Fe, Co, Ni metal ions

Mass Culture Revisited

1972 ◽  
Vol 17 (7) ◽  
pp. 406-406 ◽  
Author(s):  
EDWARD E. JONES
Keyword(s):  
Mass Culture
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