Interleukin-2 receptor beta chain gene: generation of three receptor forms by cloned human alpha and beta chain cDNA's

Science ◽  
1989 ◽  
Vol 244 (4904) ◽  
pp. 551-556 ◽  
Author(s):  
M Hatakeyama ◽  
M Tsudo ◽  
S Minamoto ◽  
T Kono ◽  
T Doi ◽  
...  
Keyword(s):  
Interleukin 2 ◽  
Chain Gene ◽  
Beta Chain ◽  
Interleukin 2 Receptor ◽  
Receptor Beta

scholarly journals Characterization of the human interleukin-2 receptor beta-chain gene promoter: regulation of promoter activity by ets gene products.

1993 ◽  
Vol 13 (10) ◽  
pp. 6201-6210 ◽  
Author(s):  
J X Lin ◽  
N K Bhat ◽  
S John ◽  
W S Queale ◽  
W J Leonard
Keyword(s):  
Binding Site ◽  
Phorbol Myristate Acetate ◽  
Promoter Activity ◽  
Interleukin 2 ◽  
Chain Gene ◽  
Beta Chain ◽  
Myristate Acetate ◽  
Interleukin 2 Receptor ◽  
Mg63 Cells ◽  
Beta Gene

The interleukin-2 receptor (IL-2R) beta chain (IL-2R beta) is an essential signaling component of high- and intermediate-affinity IL-2Rs. Our laboratory previously reported that a DNA fragment containing 857 bp of 5'-flanking sequence of the human IL-2R beta gene exhibited promoter activity. We have now further characterized the promoter and delineated cis-acting regulatory regions. The region downstream of -363 is critical for basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and contains at least three enhancer-like regions. Among them, the -56 to -34 enhancer was the most potent and had high-level activity in two T-cell lines but not in nonlymphoid HeLaS3 and MG63 cells. This enhancer contains a GGAA Ets binding site which bound two Ets family proteins, Ets-1 and GA-binding protein in vitro. Mutation of the Ets motif strongly diminished both promoter and enhancer activities. We conclude that this Ets binding site plays a key role in regulating basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and may also contribute to tissue-specific expression of the IL-2R beta gene.

Characterization of the human interleukin-2 receptor beta-chain gene promoter: regulation of promoter activity by ets gene products

1993 ◽  
Vol 13 (10) ◽  
pp. 6201-6210
Author(s):  
J X Lin ◽  
N K Bhat ◽  
S John ◽  
W S Queale ◽  
W J Leonard
Keyword(s):  
Binding Site ◽  
Phorbol Myristate Acetate ◽  
Promoter Activity ◽  
Interleukin 2 ◽  
Chain Gene ◽  
Beta Chain ◽  
Myristate Acetate ◽  
Interleukin 2 Receptor ◽  
Mg63 Cells ◽  
Beta Gene

The interleukin-2 receptor (IL-2R) beta chain (IL-2R beta) is an essential signaling component of high- and intermediate-affinity IL-2Rs. Our laboratory previously reported that a DNA fragment containing 857 bp of 5'-flanking sequence of the human IL-2R beta gene exhibited promoter activity. We have now further characterized the promoter and delineated cis-acting regulatory regions. The region downstream of -363 is critical for basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and contains at least three enhancer-like regions. Among them, the -56 to -34 enhancer was the most potent and had high-level activity in two T-cell lines but not in nonlymphoid HeLaS3 and MG63 cells. This enhancer contains a GGAA Ets binding site which bound two Ets family proteins, Ets-1 and GA-binding protein in vitro. Mutation of the Ets motif strongly diminished both promoter and enhancer activities. We conclude that this Ets binding site plays a key role in regulating basal and phorbol myristate acetate-inducible IL-2R beta promoter activity and may also contribute to tissue-specific expression of the IL-2R beta gene.

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