Ribosomal RNA Synthesis and Processing in a Particulate Site in the HeLa Cell Nucleus

Science ◽  
1966 ◽  
Vol 154 (3750) ◽  
pp. 786-789 ◽  
Author(s):  
S. Penman ◽  
I. Smith ◽  
E. Holtzman
Keyword(s):  
Hela Cell ◽  
Ribosomal Rna ◽  
Cell Nucleus ◽  
Rna Synthesis ◽  
Synthesis And Processing

The Reactivation of the Red Cell Nucleus

1967 ◽  
Vol 2 (1) ◽  
pp. 23-28 ◽  
Author(s):  
H. HARRIS
Keyword(s):  
Hela Cell ◽  
Cell Nucleus ◽  
Rna Synthesis ◽  
Direct Relationship ◽  
Nuclear Volume ◽  
Red Cell ◽  
Primary Event ◽  
Rna And Dna ◽  
Nuclear Enlargement ◽  
Made In

When the nucleus of a mature hen erythrocyte is introduced into the cytoplasm of a HeLa cell it resumes the synthesis of RNA and DNA. This reactivation of the red cell nucleus in the heterokaryon is associated with a marked increase in its volume. There is a direct relationship between the volume of the nucleus and the amount of RNA which it makes. The nuclear enlargement is not a consequence of increased RNA synthesis, or of DNA synthesis: enlargement is the primary event, and the increase in RNA synthesis is determined by it. The possibility is considered that changes in nuclear volume may regulate not only the amount of RNA made in the nucleus but also the areas of chromatin on which it is made.

Control of RNA Synthesis by Amino Acids in Landschutz Ascites Tumor Cells

1974 ◽  
Vol 52 (10) ◽  
pp. 867-876 ◽  
Author(s):  
Paul Jolicoeur ◽  
Fernand Labrie
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Inhibitory Effect ◽  
Amino Acid Starvation ◽  
Cytoplasmic Rna ◽  
Polysomal Mrna ◽  
Deficient Medium ◽  
Synthesis And Processing

Landschutz cells incubated in amino acid-deficient medium for 2.5 h show a markedly reduced incorporation of [3H]uridine into 18 S and 28 S cytoplasmic ribosomal RNA (rRNA) and into 28 S, 32 S, and 36 S nuclear RNA measured during the last 90 min of incubation, whereas the radioactivity associated with 45 S pre-rRNA is not affected. Ten-minute pulse-labeling and 15-min pulse-chase experiments show that amino acid starvation inhibits both the synthesis and processing of 45 S pre-rRNA. Amino acid starvation has no significant effect on the labeling of the nucleotide pools. This effect of amino acids was specific for rRNA since the synthesis of 4 S and 5 S cytoplasmic RNA separated on polyacrylamide gels and of polysomal mRNA analyzed on sucrose gradients was not significantly affected during amino acid starvation. These data also indicate that RNA synthesis is non-coordinated in Landschutz cells. Among the 13 amino acids essential for growth of these cells, arginine and glutamine appear to be mainly responsible for the inhibition of synthesis of 18 S and 28 S rRNA measured during incubation in complete amino acid-deficient medium. The removal of any one of the other amino acids has a small inhibitory effect on the incorporation of [3H]uridine into rRNA and their effect on the synthesis of 18 S rRNA is more pronounced than on that of 28 S rRNA. Such effect results in an unbalanced production of these two ribosomal RNA species.

scholarly journals Nuclear spreads: I. Visualization of bipartite ribosomal RNA domains.

1995 ◽  
Vol 128 (1) ◽  
pp. 15-27 ◽  
Author(s):  
M A Garcia-Blanco ◽  
D D Miller ◽  
M P Sheetz
Keyword(s):  
Ribosomal Rna ◽  
Rna Processing ◽  
Rna Synthesis ◽  
Nuclear Architecture ◽  
Strong Interactions ◽  
Synthesis And Processing ◽  
Ribosomal Rna Processing ◽  
Rna Domains ◽  
Polymerase I ◽  
Nascent Transcripts

Visualization of nuclear architecture is key to the understanding of the association between RNA synthesis and processing. This architecture is obscured by the high density of components in most nuclei. We have developed a method of spreading nuclei and nucleoli that reduces overlap of weakly associated components. Strong interactions among nuclear components are not disrupted by this method. Spread nucleoli remained structurally distinct and functionally competent in ribosomal RNA synthesis. Nascent ribosomal RNA colocalized with RNA polymerase I and fibrillarin, a protein required for processing of ribosomal RNA. Colocalization of nascent transcripts and fibrillarin was seen in nucleoli spread over several microns, suggesting a strong interaction. These data suggest that nucleoli are superassemblies of bipartite domains, each composed of a ribosomal RNA synthesis center tightly associated with areas likely to be involved in ribosomal RNA processing.

Non-isotopic mapping of ribosomal RNA synthesis and processing in the nucleolus

Chromosoma ◽  
2001 ◽  
Vol 110 (7) ◽  
pp. 460-470 ◽  
Author(s):  
David Staněk ◽  
Karel Koberna ◽  
Artem Pliss ◽  
Jan Malínský ◽  
Martin Mašata ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Synthesis And Processing

scholarly journals Accelerated rates of ribosomal RNA synthesis during growth of contracting heart cells in culture

1989 ◽  
Vol 264 (30) ◽  
pp. 18220-18227
Author(s):  
P J McDermott ◽  
L I Rothblum ◽  
S D Smith ◽  
H E Morgan
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Heart Cells ◽  
Cells In Culture ◽  
Contracting Heart
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