Regulation of ribosomal RNA synthesis and processing during inhibition of protein synthesis by 1,3-bis(2-chloroethyl)-1-nitrosourea

Biochemistry ◽  
1976 ◽  
Vol 15 (12) ◽  
pp. 2661-2668 ◽  
Author(s):  
Maria Penman ◽  
Roberta Huffman ◽  
Ajit Kumar
Keyword(s):  
Protein Synthesis ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Inhibition Of Protein Synthesis ◽  
Synthesis And Processing

Activated ribosomal RNA synthesis in regenerated rat liver upon inhibition of protein synthesis

1991 ◽  
Vol 15 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Emil H. Nikolov ◽  
Bistra B. Nankova ◽  
Mariana D. Dabeva
Keyword(s):  
Protein Synthesis ◽  
Rat Liver ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Inhibition Of Protein Synthesis

Stabilization of tubulin mRNA by inhibition of protein synthesis in sea urchin embryos

1988 ◽  
Vol 8 (8) ◽  
pp. 3518-3525
Author(s):  
Z Y Gong ◽  
B P Brandhorst
Keyword(s):  
Protein Synthesis ◽  
Sea Urchin ◽  
Mrna Stability ◽  
Rna Synthesis ◽  
Rapid Loss ◽  
Transcription Analysis ◽  
Inhibition Of Protein Synthesis ◽  
Tubulin Mrna ◽  
Autogenous Regulation

An increased level of unpolymerized tubulin caused by depolymerization of microtubules in sea urchin larvae resulted in a rapid loss of tubulin mRNA, which was prevented by nearly complete inhibition of protein synthesis. Results of an RNA run-on assay indicated that inhibition of protein synthesis does not alter tubulin gene transcription. Analysis of the decay of tubulin mRNA in embryos in which RNA synthesis was inhibited by actinomycin D indicated that inhibition of protein synthesis prevents the destabilization of tubulin mRNA. The effect was similar whether mRNA was maintained on polysomes in the presence of emetine or anisomycin or displaced from the polysomes in the presence of puromycin or pactamycin; thus, the stabilization of tubulin mRNA is not dependent on the state of the polysomes after inhibition of protein synthesis. Even after tubulin mRNA declined to a low level after depolymerization of microtubules, it could be rescued by treatment of embryos with inhibitors of protein synthesis. Tubulin mRNA could be induced to accumulate prematurely in gastrulae but not in plutei if protein synthesis was inhibited, an observation that is indicative of the importance of the autogenous regulation of tubulin mRNA stability during embryogenesis. Possible explanations for the role of protein synthesis in the control of mRNA stability are discussed.

Conditions necessary for inhibition of protein synthesis and production of cytopathic effect in Aedes albopictus cells infected with vesicular stomatitis virus

1982 ◽  
Vol 2 (1) ◽  
pp. 66-75
Author(s):  
S Gillies ◽  
V Stollar
Keyword(s):  
Protein Synthesis ◽  
Vesicular Stomatitis Virus ◽  
Aedes Albopictus ◽  
Rna Synthesis ◽  
Cytopathic Effect ◽  
Viral Gene ◽  
Inhibition Of Protein Synthesis ◽  
Infected Cells ◽  
Vesicular Stomatitis ◽  
Aedes Albopictus Cells

The relationship between the development of cytopathic effect (CPE) and the inhibition of host macromolecular synthesis was examined in a CPE-susceptible cloned line of Aedes albopictus cells after infection with vesicular stomatitis virus. To induce rapid and maximal CPE, two conditions were required: (i) presence of serum in the medium and (ii) incubation at 34 degrees C rather than at 28 degrees C. In the absence of serum, incubation of infected cultures at 34 degrees C resulted in a significant increase in viral protein and RNA synthesis compared with that observed at 28 degrees C. However, when serum was present in the medium, by 6 h after infection protein synthesis (both host and viral) was markedly inhibited when infected cells were maintained at 34 degrees C. RNA synthesis (host and viral) was also inhibited in vesicular stomatitis virus-infected cells maintained at 34 degrees C with serum, but somewhat more slowly than protein synthesis. Examination of polysome patterns indicated that when infected cultures were maintained under conditions which predispose to CPE, more than half of the ribosomes existed as monosomes, suggesting that protein synthesis was being inhibited at the level of initiation. In addition, the phosphorylation of one (or two) polysome-associated proteins was reduced when protein synthesis was inhibited. Our findings indicate a strong correlation between virus-induced CPE in the LT-C7 clone of A. albopictus cells and the inhibition of protein synthesis. Although the mechanism of the serum effect is not understood, incubation at 34 degrees C probably predisposes to CPE and inhibition of protein synthesis by increasing the amount of viral gene products made.

scholarly journals Inhibition of protein synthesis by an efficiently expressed mutation in the yeast 5.8S ribosomal RNA

1994 ◽  
Vol 22 (4) ◽  
pp. 686-693 ◽  
Author(s):  
Sherif Abou Elela ◽  
Liam Good ◽  
Yuri F. Melekhovets ◽  
Ross N. Nazar
Keyword(s):  
Protein Synthesis ◽  
Ribosomal Rna ◽  
Inhibition Of Protein Synthesis

scholarly journals Stabilization of tubulin mRNA by inhibition of protein synthesis in sea urchin embryos.

1988 ◽  
Vol 8 (8) ◽  
pp. 3518-3525 ◽  
Author(s):  
Z Y Gong ◽  
B P Brandhorst
Keyword(s):  
Protein Synthesis ◽  
Sea Urchin ◽  
Mrna Stability ◽  
Rna Synthesis ◽  
Rapid Loss ◽  
Transcription Analysis ◽  
Inhibition Of Protein Synthesis ◽  
Tubulin Mrna ◽  
Autogenous Regulation

An increased level of unpolymerized tubulin caused by depolymerization of microtubules in sea urchin larvae resulted in a rapid loss of tubulin mRNA, which was prevented by nearly complete inhibition of protein synthesis. Results of an RNA run-on assay indicated that inhibition of protein synthesis does not alter tubulin gene transcription. Analysis of the decay of tubulin mRNA in embryos in which RNA synthesis was inhibited by actinomycin D indicated that inhibition of protein synthesis prevents the destabilization of tubulin mRNA. The effect was similar whether mRNA was maintained on polysomes in the presence of emetine or anisomycin or displaced from the polysomes in the presence of puromycin or pactamycin; thus, the stabilization of tubulin mRNA is not dependent on the state of the polysomes after inhibition of protein synthesis. Even after tubulin mRNA declined to a low level after depolymerization of microtubules, it could be rescued by treatment of embryos with inhibitors of protein synthesis. Tubulin mRNA could be induced to accumulate prematurely in gastrulae but not in plutei if protein synthesis was inhibited, an observation that is indicative of the importance of the autogenous regulation of tubulin mRNA stability during embryogenesis. Possible explanations for the role of protein synthesis in the control of mRNA stability are discussed.

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