PLANT SCIENCE: Nibbling at the Plant Cell Nucleus

Science ◽  
2007 ◽  
Vol 315 (5815) ◽  
pp. 1088-1089 ◽  
Author(s):  
J. L. Dangl
Keyword(s):  
Plant Cell ◽  
Cell Nucleus ◽  
Plant Science

Histochemical techniques in plant science: more than meets the eye

2021 ◽  
Author(s):  
Vaishali Yadav ◽  
Namira Arif ◽  
Vijay Pratap Singh ◽  
Gea Guerriero ◽  
Roberto Berni ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Histochemical Staining ◽  
Cellular Level ◽  
Plant Science ◽  
Specific Cell ◽  
Environmental Constraints ◽  
Cell Wall Components ◽  
Cell Structures ◽  
Wall Components

Abstract Histochemistry is an essential analytical tool interfacing extensively with plant science. The literature is indeed constellated with examples showing its use to decipher specific physiological and developmental processes, as well as to study plant cell structures. Plant cell structures are translucent unless they are stained. Histochemistry allows the identification and localization, at the cellular level, of biomolecules and organelles in different types of cells and tissues, based on the use of specific staining reactions and imaging. Histochemical techniques are also widely used for the in-vivo localization of promoters in specific tissues, as well as to identify specific cell wall components such as lignin and polysaccharides. Histochemistry also enables the study of plants’ reactions to environmental constraints, for example, the production of reactive oxygen species (ROS) can be traced by applying histochemical staining techniques. The possibility of detecting ROS and localizing them at the cellular level is vital in establishing the mechanisms involved in the sensitivity and tolerance to different stress conditions in plants. This review comprehensively highlights the additional value of histochemistry as a complementary technique to high-throughput approaches for the study of the plant response to environmental constraints. Moreover, here we have provided and extensive survey of the available plant histochemical staining methods used for the localization of metals, minerals, secondary metabolites, cell wall components, as well as the detection of ROS production in plant cells. The use of recent technological advances like CRISPR/Cas9 based genome-editing for histological application is also addressed. This review also surveys the availale literature data on histochemical techniques used to study the response of plants to abiotic stresses and to identify the effects at the tissue and cell-level.

scholarly journals A short history of plant transformation

2019 ◽  
Author(s):  
Marc Somssich
Keyword(s):  
Transgenic Plant ◽  
Plant Cell ◽  
Plant Transformation ◽  
Plant Biotechnology ◽  
Biotechnology Industry ◽  
Plant Science ◽  
Short History ◽  
Fourth Group ◽  
History Of ◽  
Cell Genome

The 1977 discovery that Agrobacterium tumefaciens inserts a specific piece of DNA into the plant cell genome triggered a race towards the first transgenic plant. Three groups were initially involved in the race, a fourth group entered later on. This race ended in 1983 with four labs publishing their own transgenic plant cell lines. This scientific breakthrough triggered the plant-biotechnology industry, and advanced the field of plant science like hardly any other. Who won the race? Here’s 'A Short History of Plant Transformation'.

scholarly journals The DNA form of a retroviroid-like element characterized in cultivated carnation species

2001 ◽  
Vol 82 (3) ◽  
pp. 687-691 ◽  
Author(s):  
Krisztina Hegedűs ◽  
László Palkovics ◽  
Endre Kristóf Tóth ◽  
Géza Dallmann ◽  
Ervin Balázs
Keyword(s):  
Plant Cell ◽  
Cell Nucleus ◽  
Dna Sequences ◽  
Unit Length ◽  
Dianthus Caryophyllus ◽  
Shoot Proliferation ◽  
Circular Molecule ◽  
Minor Sequence ◽  
Rna And Dna ◽  
Growth Abnormality

Carnation small viroid-like RNA (CarSV RNA) is a small (275 nt), circular molecule which is unique among plant viroid-like RNAs in having a tandemly repeated homologous DNA. This DNA form was found fused to DNA sequences of carnation etched ring caulimovirus (CERV) in certain Spanish carnation plants. The observation of a growth abnormality consisting of extensive shoot proliferation in cultivated carnations in Hungary prompted the molecular analysis of these plants, in which both CarSV RNA and DNA forms were detected. Several CarSV DNA sequences were characterized in various Dianthus caryophyllus cultivars which were symptomless or showed different symptoms. CarSV DNA forms showing minor sequence heterogeneities and deletions occurred in the same plant. Unit-length CarSV DNA sequences were proven to accumulate in the plant cell nucleus. The plants studied here were not infected by any of the viruses (including CERV) or other cellular pathogens described previously in carnation.

Histones and DNA Ultrastructural Distribution in Plant Cell Nucleus: A Combination of Immunogold and Cytochemical Methods

1998 ◽  
Vol 242 (1) ◽  
pp. 45-59 ◽  
Author(s):  
P. González-Melendi ◽  
P.S. Testillano ◽  
C.G. Mena ◽  
S. Muller ◽  
I. Raska ◽  
...  
Keyword(s):  
Plant Cell ◽  
Cell Nucleus

scholarly journals The plant cell nucleus

2011 ◽  
Vol 6 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Laurent Deslandes ◽  
Susana Rivas
Keyword(s):  
Plant Cell ◽  
Cell Nucleus

Assembly and disassembly of the peripheral architecture of the plant cell nucleus during mitosis

Planta ◽  
1999 ◽  
Vol 210 (1) ◽  
pp. 165-167 ◽  
Author(s):  
Shizuka Haruyama ◽  
Kiyoshi Masuda ◽  
Kaien Fujino
Keyword(s):  
Plant Cell ◽  
Cell Nucleus
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