The Cytoplasm of the Plant Cell. A New Series of Plant Science Books, Volume VI. Alexandre Guilliermond , Lenette Rogers Atkinson

1949 ◽  
Vol 24 (2) ◽  
pp. 148-148
Author(s):  
C. P. Swanson
Keyword(s):  
Plant Cell ◽  
Plant Science

Histochemical techniques in plant science: more than meets the eye

2021 ◽  
Author(s):  
Vaishali Yadav ◽  
Namira Arif ◽  
Vijay Pratap Singh ◽  
Gea Guerriero ◽  
Roberto Berni ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Histochemical Staining ◽  
Cellular Level ◽  
Plant Science ◽  
Specific Cell ◽  
Environmental Constraints ◽  
Cell Wall Components ◽  
Cell Structures ◽  
Wall Components

Abstract Histochemistry is an essential analytical tool interfacing extensively with plant science. The literature is indeed constellated with examples showing its use to decipher specific physiological and developmental processes, as well as to study plant cell structures. Plant cell structures are translucent unless they are stained. Histochemistry allows the identification and localization, at the cellular level, of biomolecules and organelles in different types of cells and tissues, based on the use of specific staining reactions and imaging. Histochemical techniques are also widely used for the in-vivo localization of promoters in specific tissues, as well as to identify specific cell wall components such as lignin and polysaccharides. Histochemistry also enables the study of plants’ reactions to environmental constraints, for example, the production of reactive oxygen species (ROS) can be traced by applying histochemical staining techniques. The possibility of detecting ROS and localizing them at the cellular level is vital in establishing the mechanisms involved in the sensitivity and tolerance to different stress conditions in plants. This review comprehensively highlights the additional value of histochemistry as a complementary technique to high-throughput approaches for the study of the plant response to environmental constraints. Moreover, here we have provided and extensive survey of the available plant histochemical staining methods used for the localization of metals, minerals, secondary metabolites, cell wall components, as well as the detection of ROS production in plant cells. The use of recent technological advances like CRISPR/Cas9 based genome-editing for histological application is also addressed. This review also surveys the availale literature data on histochemical techniques used to study the response of plants to abiotic stresses and to identify the effects at the tissue and cell-level.

scholarly journals A short history of plant transformation

2019 ◽  
Author(s):  
Marc Somssich
Keyword(s):  
Transgenic Plant ◽  
Plant Cell ◽  
Plant Transformation ◽  
Plant Biotechnology ◽  
Biotechnology Industry ◽  
Plant Science ◽  
Short History ◽  
Fourth Group ◽  
History Of ◽  
Cell Genome

The 1977 discovery that Agrobacterium tumefaciens inserts a specific piece of DNA into the plant cell genome triggered a race towards the first transgenic plant. Three groups were initially involved in the race, a fourth group entered later on. This race ended in 1983 with four labs publishing their own transgenic plant cell lines. This scientific breakthrough triggered the plant-biotechnology industry, and advanced the field of plant science like hardly any other. Who won the race? Here’s 'A Short History of Plant Transformation'.

scholarly journals Science Forum: Vision, challenges and opportunities for a Plant Cell Atlas

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
◽  
Suryatapa Ghosh Jha ◽  
Alexander T Borowsky ◽  
Benjamin J Cole ◽  
Noah Fahlgren ◽  
...  
Keyword(s):  
Plant Cell ◽  
Current Knowledge ◽  
Cell Types ◽  
Environmental Stewardship ◽  
Plant Science ◽  
Energy Technology ◽  
Critical Component ◽  
Engineering Plant ◽  
Challenges And Opportunities ◽  
Environmental Responses

With growing populations and pressing environmental problems, future economies will be increasingly plant-based. Now is the time to reimagine plant science as a critical component of fundamental science, agriculture, environmental stewardship, energy, technology and healthcare. This effort requires a conceptual and technological framework to identify and map all cell types, and to comprehensively annotate the localization and organization of molecules at cellular and tissue levels. This framework, called the Plant Cell Atlas (PCA), will be critical for understanding and engineering plant development, physiology and environmental responses. A workshop was convened to discuss the purpose and utility of such an initiative, resulting in a roadmap that acknowledges the current knowledge gaps and technical challenges, but also underscores how the PCA initiative can help to overcome them.

scholarly journals A short history of plant transformation

2019 ◽  
Author(s):  
Marc Somssich
Keyword(s):  
Transgenic Plant ◽  
Plant Cell ◽  
Plant Transformation ◽  
Plant Biotechnology ◽  
Biotechnology Industry ◽  
Plant Science ◽  
Short History ◽  
Fourth Group ◽  
History Of ◽  
Cell Genome

The 1977 discovery that Agrobacterium tumefaciens inserts a specific piece of DNA into the plant cell genome triggered a race towards the first transgenic plant. Three groups were initially involved in the race, a fourth group entered later on. This race ended in 1983 with four labs publishing their own transgenic plant cell lines. This scientific breakthrough triggered the plant-biotechnology industry, and advanced the field of plant science like hardly any other. Who won the race? Here’s 'A Short History of Plant Transformation'.

scholarly journals A short history of plant transformation

2019 ◽  
Author(s):  
Marc Somssich
Keyword(s):  
Transgenic Plant ◽  
Plant Cell ◽  
Plant Transformation ◽  
Plant Biotechnology ◽  
Biotechnology Industry ◽  
Plant Science ◽  
Short History ◽  
Fourth Group ◽  
History Of ◽  
Cell Genome

The 1977 discovery that Agrobacterium tumefaciens inserts a specific piece of DNA into the plant cell genome triggered a race towards the first transgenic plant. Three groups were initially involved in the race, a fourth group entered later on. This race ended in 1983 with four labs publishing their own transgenic plant cell lines. This scientific breakthrough triggered the plant-biotechnology industry, and advanced the field of plant science like hardly any other. Who won the race? Here’s 'A Short History of Plant Transformation'.

PLANT SCIENCE: Nibbling at the Plant Cell Nucleus

Science ◽  
2007 ◽  
Vol 315 (5815) ◽  
pp. 1088-1089 ◽  
Author(s):  
J. L. Dangl
Keyword(s):  
Plant Cell ◽  
Cell Nucleus ◽  
Plant Science

Sectioned rubisco crystals in TEM

1990 ◽  
Vol 48 (3) ◽  
pp. 664-665
Author(s):  
Gunnel Karlsson ◽  
Jan-Olov Bovin ◽  
Michael Bosma
Keyword(s):  
Plant Cell ◽  
Carbon Fixation ◽  
Unit Cell ◽  
Protein Crystals ◽  
Unit Cell Parameters ◽  
Cell Parameters ◽  
Photosynthetic Carbon Fixation ◽  
Photosynthetic Carbon

RuBisCO (D-ribulose-l,5-biphosphate carboxylase/oxygenase) is the most aboundant enzyme in the plant cell and it catalyses the key carboxylation reaction of photosynthetic carbon fixation, but also the competing oxygenase reaction of photorespiation. In vitro crystallized RuBisCO has been studied earlier but this investigation concerns in vivo existance of RuBisCO crystals in anthers and leaves ofsugarbeets. For the identification of in vivo protein crystals it is important to be able to determinethe unit cell of cytochemically identified crystals in the same image. In order to obtain the best combination of optimal contrast and resolution we have studied different staining and electron accelerating voltages. It is known that embedding and sectioning can cause deformation and obscure the unit cell parameters.

Exploration of cell wall architecture with the rapid-freeze deep-etch technique

1993 ◽  
Vol 51 ◽  
pp. 128-129
Author(s):  
Béatrice Satiat-Jeunemaitre ◽  
Chris Hawes
Keyword(s):  
Plant Cell ◽  
Cell Walls ◽  
Cell Biology ◽  
Molecular Model ◽  
Three Dimensional ◽  
Secretory Activity ◽  
Wall Structure ◽  
Specimen Preparation ◽  
Molecular Architecture ◽  
Plant Cell Walls

The comprehension of the molecular architecture of plant cell walls is one of the best examples in cell biology which illustrates how developments in microscopy have extended the frontiers of a topic. Indeed from the first electron microscope observation of cell walls it has become apparent that our understanding of wall structure has advanced hand in hand with improvements in the technology of specimen preparation for electron microscopy. Cell walls are sub-cellular compartments outside the peripheral plasma membrane, the construction of which depends on a complex cellular biosynthetic and secretory activity (1). They are composed of interwoven polymers, synthesised independently, which together perform a number of varied functions. Biochemical studies have provided us with much data on the varied molecular composition of plant cell walls. However, the detailed intermolecular relationships and the three dimensional arrangement of the polymers in situ remains a mystery. The difficulty in establishing a general molecular model for plant cell walls is also complicated by the vast diversity in wall composition among plant species.

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