scholarly journals Real-time tracking of bacterial membrane vesicles reveals enhanced membrane traffic upon antibiotic exposure

2021 ◽  
Vol 7 (4) ◽  
pp. eabd1033
Author(s):  
Julia Bos ◽  
Luis H. Cisneros ◽  
Didier Mazel
Keyword(s):  
Real Time ◽  
Spatial Organization ◽  
Membrane Vesicles ◽  
In Vivo Experiments ◽  
Cell Clustering ◽  
Bacterial Surfaces ◽  
Real Time Tracking ◽  
Vesicle Movement ◽  
Broad Understanding

Membrane vesicles are ubiquitous carriers of molecular information. A broad understanding of the biological functions of membrane vesicles in bacteria remains elusive because of the imaging challenges during real-time in vivo experiments. Here, we provide a quantitative analysis of the motion of individual vesicles in living microbes using fluorescence microscopy, and we show that while vesicle free diffusion in the intercellular space is rare, vesicles mostly disperse along the bacterial surfaces. Most remarkably, when bacteria are challenged with low doses of antibiotics, vesicle production and traffic, quantified by instantaneous vesicle speeds and total traveled distance per unit time, are significantly enhanced. Furthermore, the enhanced vesicle movement is independent of cell clustering properties but rather is associated with a reduction of the density of surface appendages in response to antibiotics. Together, our results provide insights into the emerging field of spatial organization and dynamics of membrane vesicles in microcolonies.

Real-time tracking and dual-mode imaging of hypochlorous acid in vivo by a small-sized fluorescence probe

2021 ◽  
Vol 188 ◽  
pp. 109219
Author(s):  
Haoyang Tang ◽  
Xingyu Qiang ◽  
Ying Gao ◽  
Hao Teng ◽  
Xi Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Hypochlorous Acid ◽  
Fluorescence Probe ◽  
Dual Mode ◽  
Real Time Tracking

scholarly journals Real-Time Optical Monitoring of Endotracheal Tube Displacement

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 174
Author(s):  
Ramzan Ullah ◽  
Karl Doerfer ◽  
Pawjai Khampang ◽  
Faraneh Fathi ◽  
Wenzhou Hong ◽  
...  
Keyword(s):  
Endotracheal Tube ◽  
Real Time ◽  
Near Infrared ◽  
Ex Vivo ◽  
Light Emitting Diode ◽  
Clinical Care ◽  
Cost Effective ◽  
Infrared Light ◽  
In Vivo Experiments

Proper ventilation of a patient with an endotracheal tube (ETT) requires proper placement of the ETT. We present a sensitive, noninvasive, operator-free, and cost-effective optical sensor, called Opt-ETT, for the real-time assessment of ETT placement and alerting of the clinical care team should the ETT become displaced. The Opt-ETT uses a side-firing optical fiber, a near-infrared light-emitting diode, two photodetectors with an integrated amplifier, an Arduino board, and a computer loaded with a custom LabVIEW program to monitor the position of the endotracheal tube inside the windpipe. The Opt-ETT generates a visual and audible warning if the tube moves over a distance set by the operator. Displacement prediction is made using a second-order polynomial fit to the voltages measured from each detector. The system is tested on ex vivo porcine tissues, and the accuracy is determined to be better than 1.0 mm. In vivo experiments with a pig are conducted to test the performance and usability of the system.

scholarly journals Loading Graphene Quantum Dots into Optical-Magneto Nanoparticles for Real-Time Tracking In Vivo

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2191 ◽  
Author(s):  
Yu Wang ◽  
Nan Xu ◽  
Yongkai He ◽  
Jingyun Wang ◽  
Dan Wang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Real Time ◽  
Fluorescent Dyes ◽  
Graphene Quantum Dots ◽  
Shell Nanoparticles ◽  
One Pot ◽  
Real Time Tracking

Fluorescence imaging offers a new approach to visualize real-time details on a cellular level in vitro and in vivo without radioactive damage. Poor light stability of organic fluorescent dyes makes long-term imaging difficult. Due to their outstanding optical properties and unique structural features, graphene quantum dots (GQDs) are promising in the field of imaging for real-time tracking in vivo. At present, GQDs are mainly loaded on the surface of nanoparticles. In this study, we developed an efficient and convenient one-pot method to load GQDs into nanoparticles, leading to longer metabolic processes in blood and increased delivery of GQDs to tumors. Optical-magneto ferroferric oxide@polypyrrole (Fe3O4@PPy) core-shell nanoparticles were chosen for their potential use in cancer therapy. The in vivo results demonstrated that by loading GQDs, it was possible to monitor the distribution and metabolism of nanoparticles. This study provided new insights into the application of GQDs in long-term in vivo real-time tracking.

In vivo Real-time Tracking of Single Quantum Dots Conjugated with Monoclonal Anti-HER2 Antibody in Tumors of Mice

2007 ◽  
Vol 67 (3) ◽  
pp. 1138-1144 ◽  
Author(s):  
Hiroshi Tada ◽  
Hideo Higuchi ◽  
Tomonobu M. Wanatabe ◽  
Noriaki Ohuchi
Keyword(s):  
Quantum Dots ◽  
Real Time ◽  
Single Quantum ◽  
Single Quantum Dots ◽  
Real Time Tracking

scholarly journals Expandable and implantable bioelectronic complex for analyzing and regulating real-time activity of the urinary bladder

2020 ◽  
Vol 6 (46) ◽  
pp. eabc9675
Author(s):  
Tae-Min Jang ◽  
Joong Hoon Lee ◽  
Honglei Zhou ◽  
Jaesun Joo ◽  
Bong Hee Lim ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Real Time ◽  
Detrusor Underactivity ◽  
Seamless Integration ◽  
Time Activity ◽  
Time Dynamic ◽  
In Vivo Experiments ◽  
Urinary Infections ◽  
Life Threatening

Underactive bladder or detrusor underactivity (DUA), that is, not being able to micturate, has received less attention with little research and remains unknown or limited on pathological causes and treatments as opposed to overactive bladder, although the syndrome may pose a risk of urinary infections or life-threatening kidney damage. Here, we present an integrated expandable electronic and optoelectronic complex that behaves as a single body with the elastic, time-dynamic urinary bladder with substantial volume changes up to ~300%. The system configuration of the electronics validated by the theoretical model allows conformal, seamless integration onto the urinary bladder without a glue or suture, enabling precise monitoring with various electrical components for real-time status and efficient optogenetic manipulation for urination at the desired time. In vivo experiments using diabetic DUA models demonstrate the possibility for practical uses of high-fidelity electronics in clinical trials associated with the bladder and other elastic organs.

A selective and sensitive near-infrared fluorescent probe for in vivo real time tracking of exogenous and metabolized hydrazine, a genotoxic impurity

2020 ◽  
Vol 8 (45) ◽  
pp. 10353-10359
Author(s):  
Shun Wang ◽  
Jian Liu ◽  
Linjiang Song ◽  
Qingrong Qi ◽  
Zicheng Li ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Real Time Tracking

The hydrazine level in the liver and kidneys of mice after administration of isoniazid was monitored by using probe Hcy-DB.

Photocaged prodrug under NIR light-triggering with dual-channel fluorescence: in vivo real-time tracking for precise drug delivery

2018 ◽  
Vol 61 (10) ◽  
pp. 1293-1300 ◽  
Author(s):  
Zhiqian Guo ◽  
Yaguang Ma ◽  
Yajing Liu ◽  
Chenxu Yan ◽  
Ping Shi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Real Time ◽  
Dual Channel ◽  
Nir Light ◽  
Real Time Tracking
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