scholarly journals Expandable and implantable bioelectronic complex for analyzing and regulating real-time activity of the urinary bladder

2020 ◽  
Vol 6 (46) ◽  
pp. eabc9675
Author(s):  
Tae-Min Jang ◽  
Joong Hoon Lee ◽  
Honglei Zhou ◽  
Jaesun Joo ◽  
Bong Hee Lim ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Real Time ◽  
Detrusor Underactivity ◽  
Seamless Integration ◽  
Time Activity ◽  
Time Dynamic ◽  
In Vivo Experiments ◽  
Urinary Infections ◽  
Life Threatening

Underactive bladder or detrusor underactivity (DUA), that is, not being able to micturate, has received less attention with little research and remains unknown or limited on pathological causes and treatments as opposed to overactive bladder, although the syndrome may pose a risk of urinary infections or life-threatening kidney damage. Here, we present an integrated expandable electronic and optoelectronic complex that behaves as a single body with the elastic, time-dynamic urinary bladder with substantial volume changes up to ~300%. The system configuration of the electronics validated by the theoretical model allows conformal, seamless integration onto the urinary bladder without a glue or suture, enabling precise monitoring with various electrical components for real-time status and efficient optogenetic manipulation for urination at the desired time. In vivo experiments using diabetic DUA models demonstrate the possibility for practical uses of high-fidelity electronics in clinical trials associated with the bladder and other elastic organs.

scholarly journals Real-Time Expanded Field-of-View for Minimally Invasive Surgery Using Multi-Camera Visual Simultaneous Localization and Mapping

Sensors ◽  
2021 ◽  
Vol 21 (6) ◽  
pp. 2106
Author(s):  
Ahmed Afifi ◽  
Chisato Takada ◽  
Yuichiro Yoshimura ◽  
Toshiya Nakaguchi
Keyword(s):  
Minimally Invasive Surgery ◽  
Minimally Invasive ◽  
Real Time ◽  
Invasive Surgery ◽  
Simultaneous Localization And Mapping ◽  
Field Of View ◽  
Time Dynamic ◽  
Matrix Estimation ◽  
Localization And Mapping

Minimally invasive surgery is widely used because of its tremendous benefits to the patient. However, there are some challenges that surgeons face in this type of surgery, the most important of which is the narrow field of view. Therefore, we propose an approach to expand the field of view for minimally invasive surgery to enhance surgeons’ experience. It combines multiple views in real-time to produce a dynamic expanded view. The proposed approach extends the monocular Oriented features from an accelerated segment test and Rotated Binary robust independent elementary features—Simultaneous Localization And Mapping (ORB-SLAM) to work with a multi-camera setup. The ORB-SLAM’s three parallel threads, namely tracking, mapping and loop closing, are performed for each camera and new threads are added to calculate the relative cameras’ pose and to construct the expanded view. A new algorithm for estimating the optimal inter-camera correspondence matrix from a set of corresponding 3D map points is presented. This optimal transformation is then used to produce the final view. The proposed approach was evaluated using both human models and in vivo data. The evaluation results of the proposed correspondence matrix estimation algorithm prove its ability to reduce the error and to produce an accurate transformation. The results also show that when other approaches fail, the proposed approach can produce an expanded view. In this work, a real-time dynamic field-of-view expansion approach that can work in all situations regardless of images’ overlap is proposed. It outperforms the previous approaches and can also work at 21 fps.

scholarly journals Real-Time Optical Monitoring of Endotracheal Tube Displacement

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 174
Author(s):  
Ramzan Ullah ◽  
Karl Doerfer ◽  
Pawjai Khampang ◽  
Faraneh Fathi ◽  
Wenzhou Hong ◽  
...  
Keyword(s):  
Endotracheal Tube ◽  
Real Time ◽  
Near Infrared ◽  
Ex Vivo ◽  
Light Emitting Diode ◽  
Clinical Care ◽  
Cost Effective ◽  
Infrared Light ◽  
In Vivo Experiments

Proper ventilation of a patient with an endotracheal tube (ETT) requires proper placement of the ETT. We present a sensitive, noninvasive, operator-free, and cost-effective optical sensor, called Opt-ETT, for the real-time assessment of ETT placement and alerting of the clinical care team should the ETT become displaced. The Opt-ETT uses a side-firing optical fiber, a near-infrared light-emitting diode, two photodetectors with an integrated amplifier, an Arduino board, and a computer loaded with a custom LabVIEW program to monitor the position of the endotracheal tube inside the windpipe. The Opt-ETT generates a visual and audible warning if the tube moves over a distance set by the operator. Displacement prediction is made using a second-order polynomial fit to the voltages measured from each detector. The system is tested on ex vivo porcine tissues, and the accuracy is determined to be better than 1.0 mm. In vivo experiments with a pig are conducted to test the performance and usability of the system.

scholarly journals Real-Time Dynamic Imaging of Virus Distribution In Vivo

PLoS ONE ◽  
2011 ◽  
Vol 6 (2) ◽  
pp. e17076 ◽  
Author(s):  
Sean E. Hofherr ◽  
Kristen E. Adams ◽  
Christopher Y. Chen ◽  
Shannon May ◽  
Eric A. Weaver ◽  
...  
Keyword(s):  
Real Time ◽  
Dynamic Imaging ◽  
Time Dynamic ◽  
Virus Distribution

CREATION OF CLINICALLY-DIFFERENTIAL TUMOR MIMIC MODEL USING VASELINE-BASED MATERIALS WITH BARIUM SULFATE FOR THE VALIDATION OF REAL-TIME ULTRASOUND IMAGE-GUIDED LIVER BIOPSY SYSTEM

2016 ◽  
Vol 28 (01) ◽  
pp. 1650003
Author(s):  
Cheng Li ◽  
Jin Yao Teo ◽  
Jiaze Wu ◽  
Apoorva Gogna ◽  
Bien Soo Tan ◽  
...  
Keyword(s):  
Liver Biopsy ◽  
Real Time ◽  
Barium Sulfate ◽  
Ex Vivo ◽  
Ultrasound Image ◽  
Tumor Model ◽  
Palpable Mass ◽  
Image Guided ◽  
In Vivo Experiments

Testing objects are important for the validation of developing biopsy systems. Unfortunately, they are very hard to obtain. Motivated by this issue, the purpose of this study is to develop a technique for the easy creation of a model to simulate tumors of different sizes inside porcine livers, which could be used for ultrasound image-guided liver biopsy amongst other applications, and evaluate its performance by comparing to the more widely-used approaches in-vivo and ex-vivo. In this study, a Vaseline-based tumor model, and a more widely-used agar-based tumor model to provide comparison with the proposed method were created and injected into porcine livers as biopsy targets. The clinician located simulated tumors using real-time 2D imaging under the guidance of a robotic arm to delivery the biopsy in ex-vivo and in-vivo experiments. The results show that the optimum tumor model was created from a mixture of Vaseline, glycerol, and barium sulfate which can be easily produced and injected. All Vaseline-based simulated tumors were of solid, palpable mass on gross examination, and ultrasound imaging revealed clearly visible lesions. The clinician successfully performed ultrasound image guided liver biopsy in all the trials (10/10) in the ex-vivo experiment, and 2 out of 3 trials (2/3) in the in-vivo experiment on this optimum tumor model. We described a novel technique of creating solid liver tumor models that can be used for ultrasound image-guided liver biopsy.

scholarly journals Real-time tracking of bacterial membrane vesicles reveals enhanced membrane traffic upon antibiotic exposure

2021 ◽  
Vol 7 (4) ◽  
pp. eabd1033
Author(s):  
Julia Bos ◽  
Luis H. Cisneros ◽  
Didier Mazel
Keyword(s):  
Real Time ◽  
Spatial Organization ◽  
Membrane Vesicles ◽  
In Vivo Experiments ◽  
Cell Clustering ◽  
Bacterial Surfaces ◽  
Real Time Tracking ◽  
Vesicle Movement ◽  
Broad Understanding

Membrane vesicles are ubiquitous carriers of molecular information. A broad understanding of the biological functions of membrane vesicles in bacteria remains elusive because of the imaging challenges during real-time in vivo experiments. Here, we provide a quantitative analysis of the motion of individual vesicles in living microbes using fluorescence microscopy, and we show that while vesicle free diffusion in the intercellular space is rare, vesicles mostly disperse along the bacterial surfaces. Most remarkably, when bacteria are challenged with low doses of antibiotics, vesicle production and traffic, quantified by instantaneous vesicle speeds and total traveled distance per unit time, are significantly enhanced. Furthermore, the enhanced vesicle movement is independent of cell clustering properties but rather is associated with a reduction of the density of surface appendages in response to antibiotics. Together, our results provide insights into the emerging field of spatial organization and dynamics of membrane vesicles in microcolonies.

scholarly journals Poly-L-lysine as an Effective and Safe Desquamation Inducer of Urinary Bladder Epithelium

Polymers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1506 ◽  
Author(s):  
Mojca Kerec Kos ◽  
Peter Veranič ◽  
Andreja Erman
Keyword(s):  
Urinary Bladder ◽  
Large Scale ◽  
Structural Changes ◽  
Structural Integrity ◽  
Ex Vivo ◽  
Urothelial Cells ◽  
Bladder Epithelium ◽  
Epithelial Regeneration ◽  
In Vivo Experiments

Induced desquamation of urinary bladder epithelial cells, also called urothelial cells, is frequently used in studies of bladder epithelial regeneration and also in treating recurrent bacterial cystitis. Positively charged polymer chitosan is known to cause large-scale desquamation of terminally differentiated urothelial cells called umbrella cells. Aiming to compare the desquamation ability of another polycation poly-L-lysine, we studied the effect of this polymer on the functional and structural integrity of the urothelium in ex vivo and in vivo experiments. The urothelium was analyzed by measuring transepithelial electrical resistance, and the structural changes of its luminal surface were analyzed with scanning electron microscopy. The results revealed a selective and concentration-dependent desquamation effect of poly-L-lysine on superficial urothelial cells followed by quick regeneration of the urothelium, which functionally and structurally recovers in 2 to 3 h after poly-L-lysine–induced injury. Poly-L-lysine was thus proven to be a promising polymer to be used when desquamation of urothelial cells is required in basic and potentially clinical studies.

Control Model Constructing on Real-Time Dynamic Reversible Lane in Condition of Connected Vehicle

CICTP 2020 ◽  
2020 ◽  
Author(s):  
Lina Mao ◽  
Wenquan Li ◽  
Pengsen Hu ◽  
Guiliang Zhou ◽  
Huiting Zhang ◽  
...  
Keyword(s):  
Real Time ◽  
Control Model ◽  
Connected Vehicle ◽  
Time Dynamic
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