scholarly journals Regional and correlative sweat analysis using high-throughput microfluidic sensing patches toward decoding sweat

2019 ◽  
Vol 5 (8) ◽  
pp. eaaw9906 ◽  
Author(s):  
Hnin Yin Yin Nyein ◽  
Mallika Bariya ◽  
Liisa Kivimäki ◽  
Sanna Uusitalo ◽  
Tiffany Sun Liaw ◽  
...  
Keyword(s):  
High Throughput ◽  
Sweat Rate ◽  
Point Of Care ◽  
Wearable Sensors ◽  
Whole Body ◽  
Roll To Roll ◽  
Real Time Measurement ◽  
Chemically Induced ◽  
Sweat Testing ◽  
Glucose Dynamics

Recent technological advancements in wearable sensors have made it easier to detect sweat components, but our limited understanding of sweat restricts its application. A critical bottleneck for temporal and regional sweat analysis is achieving uniform, high-throughput fabrication of sweat sensor components, including microfluidic chip and sensing electrodes. To overcome this challenge, we introduce microfluidic sensing patches mass fabricated via roll-to-roll (R2R) processes. The patch allows sweat capture within a spiral microfluidic for real-time measurement of sweat parameters including [Na+], [K+], [glucose], and sweat rate in exercise and chemically induced sweat. The patch is demonstrated for investigating regional sweat composition, predicting whole-body fluid/electrolyte loss during exercise, uncovering relationships between sweat metrics, and tracking glucose dynamics to explore sweat-to-blood correlations in healthy and diabetic individuals. By enabling a comprehensive sweat analysis, the presented device is a crucial tool for advancing sweat testing beyond the research stage for point-of-care medical and athletic applications.

scholarly journals High-throughput roll-to-roll production of polymer biochips for multiplexed DNA detection in point-of-care diagnostics

Lab on a Chip ◽  
2020 ◽  
Vol 20 (22) ◽  
pp. 4106-4117
Author(s):  
Pelin Toren ◽  
Martin Smolka ◽  
Anja Haase ◽  
Ursula Palfinger ◽  
Dieter Nees ◽  
...  
Keyword(s):  
High Throughput ◽  
Nanoimprint Lithography ◽  
Dna Detection ◽  
Point Of Care ◽  
Roll To Roll ◽  
Flexible Polymer ◽  
Nano Structures ◽  
Point Of Care Diagnostics ◽  
Polymer Foils

Roll-to-roll UV nanoimprint lithography has superior advantages for high-throughput manufacturing of micro- or nano-structures on flexible polymer foils with various geometries and configurations.

3D Printed Smart Silk Wearable Sensors

The Analyst ◽  
2021 ◽  
Author(s):  
Tianshu Chu ◽  
Huili Wang ◽  
Yumeng Qiu ◽  
Haoxi Luo ◽  
Bingfang He ◽  
...  
Keyword(s):  
Point Of Care ◽  
Wearable Sensors ◽  
Point Of Care Testing ◽  
Biochemical Sensing ◽  
3D Printed ◽  
Physiological And Biochemical

Wearable sensors play a key role in point-of-care testing (POCT) for its flexible and integration capability on sensitive physiological and biochemical sensing. Here, we present a multifunction wearable silk patch...

scholarly journals INSIGHT: A population-scale COVID-19 testing strategy combining point-of-care diagnosis with centralized high-throughput sequencing

2021 ◽  
Vol 7 (7) ◽  
pp. eabe5054
Author(s):  
Qianxin Wu ◽  
Chenqu Suo ◽  
Tom Brown ◽  
Tengyao Wang ◽  
Sarah A. Teichmann ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Population Level ◽  
Reaction Products ◽  
Testing Strategy ◽  
Asymptomatic Patients ◽  
Testing Stage ◽  
Population Scale

We present INSIGHT [isothermal NASBA (nucleic acid sequence–based amplification) sequencing–based high-throughput test], a two-stage coronavirus disease 2019 testing strategy, using a barcoded isothermal NASBA reaction. It combines point-of-care diagnosis with next-generation sequencing, aiming to achieve population-scale testing. Stage 1 allows a quick decentralized readout for early isolation of presymptomatic or asymptomatic patients. It gives results within 1 to 2 hours, using either fluorescence detection or a lateral flow readout, while simultaneously incorporating sample-specific barcodes. The same reaction products from potentially hundreds of thousands of samples can then be pooled and used in a highly multiplexed sequencing–based assay in stage 2. This second stage confirms the near-patient testing results and facilitates centralized data collection. The 95% limit of detection is <50 copies of viral RNA per reaction. INSIGHT is suitable for further development into a rapid home-based, point-of-care assay and is potentially scalable to the population level.

scholarly journals Triboelectric Effect Enabled Self-Powered, Point-of-Care Diagnostics: Opportunities for Developing ASSURED and REASSURED Devices

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 337
Author(s):  
Navneet Soin ◽  
Sam J. Fishlock ◽  
Colin Kelsey ◽  
Suzanne Smith
Keyword(s):  
High Efficiency ◽  
Resource Constraints ◽  
Point Of Care ◽  
Wearable Sensors ◽  
Middle Income ◽  
Physiological Signal ◽  
Point Of Care Diagnostics ◽  
Self Powered ◽  
Triboelectric Nanogenerators ◽  
Triboelectric Effect

The use of rapid point-of-care (PoC) diagnostics in conjunction with physiological signal monitoring has seen tremendous progress in their availability and uptake, particularly in low- and middle-income countries (LMICs). However, to truly overcome infrastructural and resource constraints, there is an urgent need for self-powered devices which can enable on-demand and/or continuous monitoring of patients. The past decade has seen the rapid rise of triboelectric nanogenerators (TENGs) as the choice for high-efficiency energy harvesting for developing self-powered systems as well as for use as sensors. This review provides an overview of the current state of the art of such wearable sensors and end-to-end solutions for physiological and biomarker monitoring. We further discuss the current constraints and bottlenecks of these devices and systems and provide an outlook on the development of TENG-enabled PoC/monitoring devices that could eventually meet criteria formulated specifically for use in LMICs.

scholarly journals Toward High‐Throughput Texturing of Polymer Foils for Enhanced Light Trapping in Flexible Perovskite Solar Cells Using Roll‐to‐Roll Hot Embossing

2020 ◽  
Vol 22 (4) ◽  
pp. 1901217 ◽  
Author(s):  
Marcos Soldera ◽  
Qiong Wang ◽  
Flavio Soldera ◽  
Valentin Lang ◽  
Antonio Abate ◽  
...  
Keyword(s):  
Solar Cells ◽  
High Throughput ◽  
Light Trapping ◽  
Perovskite Solar Cells ◽  
Hot Embossing ◽  
Roll To Roll ◽  
Polymer Foils

scholarly journals HIGH-THROUGHPUT ROLL-TO-ROLL PRODUCTION OF BIO-FUNCTIONALIZED POLYMER COMPONENTS

2020 ◽  
Vol 5 (1) ◽  
pp. 11-16
Author(s):  
Pelin TOREN ◽  
◽  
Markus RUMPLER ◽  
Martin SMOLKA ◽  
Anja HAASE ◽  
...  
Keyword(s):  
High Throughput ◽  
Roll To Roll

Influences of overall thermal balance on local inputs for drive of evaporation in men

1987 ◽  
Vol 62 (3) ◽  
pp. 926-931 ◽  
Author(s):  
M. Heising ◽  
J. Werner
Keyword(s):  
Thermal Load ◽  
Sweat Rate ◽  
Thermal Balance ◽  
Whole Body ◽  
Thermal Loads ◽  
Climatic Chamber ◽  
Local Skin ◽  
Local Skin Temperature ◽  
Local Mean ◽  
Regulatory Models

Three kinds of experiments were carried out in a climatic chamber: experiments with warm load on the whole body at 36 degrees C (4 subjects); experiments at 36 degrees C with reduction of thermal load (28 degrees C) on the left leg (right leg at 36 degrees C) (8 subjects); and experiments at 36 degrees C with antisymmetric thermal load on the legs of 44 degrees C (right leg) and 28 degrees C (left leg), which resulted in additional thermal loads of +/- 30 W/leg (8 subjects). The additional thermal loads, which were applied via two climatic boxes, produced measurable effects on sweat rate when applied to one leg only. In comparison to the experiment 1, experiment 2 brought about a significant reduction of local evaporation on the left leg. With antisymmetric thermal loads on both legs (experiment 3), which did not influence the overall thermal balance, there was no significant influence on local evaporation, although significant changes of local temperatures were measured. It is suggested that the well-known regulatory models, declaring local, mean skin, and core temperatures as local evaporation drive should be supplemented with an important additional feature: local control of evaporation by local skin temperature may be blocked by an overall thermal balance.

Forearm cutaneous vascular and sudomotor responses to whole body passive heat stress in young smokers

2015 ◽  
Vol 309 (1) ◽  
pp. R36-R42 ◽  
Author(s):  
Nicole E. Moyen ◽  
Hannah M. Anderson ◽  
Jenna M. Burchfield ◽  
Matthew A. Tucker ◽  
Melina A. Gonzalez ◽  
...  
Keyword(s):  
Heat Stress ◽  
Sweat Gland ◽  
Sweat Rate ◽  
Whole Body ◽  
Mean Body Temperature ◽  
Doppler Flowmetry ◽  
Cutaneous Vasodilation ◽  
Local Sweat Rate ◽  
Vasomotor Activity ◽  
Young Smokers

The purpose of this study was to compare smokers and nonsmokers' sudomotor and cutaneous vascular responses to whole body passive heat stress. Nine regularly smoking (SMK: 29 ± 9 yr; 10 ± 6 cigarettes/day) and 13 nonsmoking (N-SMK: 27 ± 8 yr) males were passively heated until core temperature (TC) increased 1.5°C from baseline. Forearm local sweat rate (LSR) via ventilated capsule, sweat gland activation (SGA), sweat gland output (SGO), and cutaneous vasomotor activity via laser-Doppler flowmetry (CVC) were measured as mean body temperature increased (ΔTb) during passive heating using a water-perfused suit. Compared with N-SMK, SMK had a smaller ΔTb at the onset of sweating (0.52 ± 0.19 vs. 0.35 ± 0.14°C, respectively; P = 0.03) and cutaneous vasodilation (0.61 ± 0.21 vs. 0.31 ± 0.12°C, respectively; P < 0.01). Increases in LSR and CVC per °C ΔTb (i.e., sensitivity) were similar in N-SMK and SMK (LSR: 0.63 ± 0.21 vs. 0.60 ± 0.40 Δmg/cm2/min/°C ΔTb, respectively, P = 0.81; CVC: 82.5 ± 46.2 vs. 58.9 ± 23.3 Δ%max/°C ΔTb, respectively; P = 0.19). However, the plateau in LSR during whole body heating was higher in N-SMK vs. SMK (1.00 ± 0.13 vs. 0.79 ± 0.26 mg·cm−2·min−1; P = 0.03), which was likely a result of higher SGO (8.94 ± 3.99 vs. 5.94 ± 3.49 μg·gland−1·min−1, respectively; P = 0.08) and not number of SGA (104 ± 7 vs. 121 ± 9 glands/cm2, respectively; P = 0.58). During whole body passive heat stress, smokers had an earlier onset for forearm sweating and cutaneous vasodilation, but a lower local sweat rate that was likely due to lower sweat output per gland. These data provide insight into local (i.e., forearm) thermoregulatory responses of young smokers during uncompensatory whole body passive heat stress.

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