scholarly journals Writing in the granular gel medium

2015 ◽  
Vol 1 (8) ◽  
pp. e1500655 ◽  
Author(s):  
Tapomoy Bhattacharjee ◽  
Steven M. Zehnder ◽  
Kyle G. Rowe ◽  
Suhani Jain ◽  
Ryan M. Nixon ◽  
...  
Keyword(s):  
Flexible Electronics ◽  
Smart Materials ◽  
Three Dimensional ◽  
Polymeric Materials ◽  
Living Cells ◽  
Particle Diffusion ◽  
Particle Engineering ◽  
3D Objects ◽  
Solid States ◽  
Closed Shells

Gels made from soft microscale particles smoothly transition between the fluid and solid states, making them an ideal medium in which to create macroscopic structures with microscopic precision. While tracing out spatial paths with an injection tip, the granular gel fluidizes at the point of injection and then rapidly solidifies, trapping injected material in place. This physical approach to creating three-dimensional (3D) structures negates the effects of surface tension, gravity, and particle diffusion, allowing a limitless breadth of materials to be written. With this method, we used silicones, hydrogels, colloids, and living cells to create complex large aspect ratio 3D objects, thin closed shells, and hierarchically branched tubular networks. We crosslinked polymeric materials and removed them from the granular gel, whereas uncrosslinked particulate systems were left supported within the medium for long times. This approach can be immediately used in diverse areas, contributing to tissue engineering, flexible electronics, particle engineering, smart materials, and encapsulation technologies.

scholarly journals Current Advances in 3D Bioprinting Technology and Its Applications for Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2958
Author(s):  
JunJie Yu ◽  
Su A Park ◽  
Wan Doo Kim ◽  
Taeho Ha ◽  
Yuan-Zhu Xin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Cell Density ◽  
Tissue Regeneration ◽  
Three Dimensional ◽  
Living Cells ◽  
3D Bioprinting ◽  
3D Objects ◽  
Advantages And Disadvantages ◽  
Resolution Cell

Three-dimensional (3D) bioprinting technology has emerged as a powerful biofabrication platform for tissue engineering because of its ability to engineer living cells and biomaterial-based 3D objects. Over the last few decades, droplet-based, extrusion-based, and laser-assisted bioprinters have been developed to fulfill certain requirements in terms of resolution, cell viability, cell density, etc. Simultaneously, various bio-inks based on natural–synthetic biomaterials have been developed and applied for successful tissue regeneration. To engineer more realistic artificial tissues/organs, mixtures of bio-inks with various recipes have also been developed. Taken together, this review describes the fundamental characteristics of the existing bioprinters and bio-inks that have been currently developed, followed by their advantages and disadvantages. Finally, various tissue engineering applications using 3D bioprinting are briefly introduced.

Fabrication of 3D Temperature Sensor Using Magnetostrictive Inkjet Printhead

2020 ◽  
Vol 64 (5) ◽  
pp. 50405-1-50405-5
Author(s):  
Young-Woo Park ◽  
Myounggyu Noh
Keyword(s):  
Flexible Electronics ◽  
Inkjet Printing ◽  
Temperature Sensor ◽  
Computer Aided Design ◽  
Low Cost ◽  
Three Dimensional ◽  
Drop On Demand ◽  
Aided Design ◽  
Nanoparticle Ink ◽  
Inkjet Printhead

Abstract Recently, the three-dimensional (3D) printing technique has attracted much attention for creating objects of arbitrary shape and manufacturing. For the first time, in this work, we present the fabrication of an inkjet printed low-cost 3D temperature sensor on a 3D-shaped thermoplastic substrate suitable for packaging, flexible electronics, and other printed applications. The design, fabrication, and testing of a 3D printed temperature sensor are presented. The sensor pattern is designed using a computer-aided design program and fabricated by drop-on-demand inkjet printing using a magnetostrictive inkjet printhead at room temperature. The sensor pattern is printed using commercially available conductive silver nanoparticle ink. A moving speed of 90 mm/min is chosen to print the sensor pattern. The inkjet printed temperature sensor is demonstrated, and it is characterized by good electrical properties, exhibiting good sensitivity and linearity. The results indicate that 3D inkjet printing technology may have great potential for applications in sensor fabrication.

Autonomous Operation of 3D DNA Walkers in Living Cells for MicroRNA Imaging

Nanoscale ◽  
2021 ◽  
Author(s):  
Hui Hu ◽  
Fu Zhou ◽  
Baojuan Wang ◽  
Xin Chang ◽  
Tianyue Dai ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Three Dimensional ◽  
Living Cells ◽  
Autonomous Operation ◽  
Dna Walkers

Three dimensional (3D) DNA walkers possesses the potential as ideal candidates for signal transduction and amplification in bioassays. However, intracellularly autonomous operation of 3D DNA walkers is still limitedly implemented...

scholarly journals 2P-022 Investigating protein three-dimensional structures inside living cells by in-cell NMR spectroscopy(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S78
Author(s):  
Junpei Hamatsu ◽  
Daisuke Sakakibara ◽  
Atsuko Sasaki ◽  
Teppei Ikeya ◽  
Masaki Mishima ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Annual Meeting ◽  
Three Dimensional ◽  
Living Cells ◽  
Biophysical Society

ELEMENT-FREE METHODS VS. MESH-LESS CAE

2006 ◽  
Vol 03 (04) ◽  
pp. 445-464 ◽  
Author(s):  
HIDEYUKI SAKURAI
Keyword(s):  
Mesh Generation ◽  
Flow Analysis ◽  
Three Dimensional ◽  
Generation Task ◽  
3D Objects ◽  
Computer Aided ◽  
Analysis System ◽  
Mesh Less ◽  
Element Free ◽  
Cae System

Element-free methods (EFreeMs) are expected to eliminate the mesh generation task. However, a computer aided engineering (CAE) system by EFreeM for complex three-dimensional (3D) objects has not yet been developed. This paper discusses the obstacles to the CAE and way to solve them. A 3D groundwater flow analysis system with an EFreeM is presented as a practical CAE. In the system, instead of pursuing mesh-less CAE, a unique mesh is employed to achieve the practical CAE. Some 3D examples show the performance and usefulness of the system. Two serious drawbacks of the EFreeM are also discussed from the viewpoint of the practical CAE.

New method of holographic three-dimensional tracking of living cells exploiting their morphological properties

2013 ◽  
Author(s):  
Pasquale Memmolo ◽  
Maria Iannone ◽  
Maurizio Ventre ◽  
Paolo Antonio Netti ◽  
Andrea Finizio ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
New Method ◽  
Morphological Properties

scholarly journals Developmental enhancers and chromosome topology

Science ◽  
2018 ◽  
Vol 361 (6409) ◽  
pp. 1341-1345 ◽  
Author(s):  
Eileen E. M. Furlong ◽  
Michael Levine
Keyword(s):  
Three Dimensional ◽  
Cell Types ◽  
Living Cells ◽  
Specific Cell ◽  
Transcriptional Dynamics ◽  
Spatiotemporal Expression ◽  
Chromosome Topology ◽  
Classical Models ◽  
And Function ◽  
Target Promoters

Developmental enhancers mediate on/off patterns of gene expression in specific cell types at particular stages during metazoan embryogenesis. They typically integrate multiple signals and regulatory determinants to achieve precise spatiotemporal expression. Such enhancers can map quite far—one megabase or more—from the genes they regulate. How remote enhancers relay regulatory information to their target promoters is one of the central mysteries of genome organization and function. A variety of contrasting mechanisms have been proposed over the years, including enhancer tracking, linking, looping, and mobilization to transcription factories. We argue that extreme versions of these mechanisms cannot account for the transcriptional dynamics and precision seen in living cells, tissues, and embryos. We describe emerging evidence for dynamic three-dimensional hubs that combine different elements of the classical models.

scholarly journals Supercritical carbon dioxide design strategies: from drug carriers to soft killers

2015 ◽  
Vol 373 (2057) ◽  
pp. 20150009 ◽  
Author(s):  
Ana Aguiar-Ricardo ◽  
Vasco D. B. Bonifácio ◽  
Teresa Casimiro ◽  
Vanessa G. Correia
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Three Dimensional ◽  
Polymeric Materials ◽  
Drug Carriers ◽  
Water Soluble ◽  
Materials Processing ◽  
Design Strategies ◽  
Simple Economic ◽  
Supercritical Carbon

The integrated use of supercritical carbon dioxide (scCO 2 ) and micro- and nanotechnologies has enabled new sustainable strategies for the manufacturing of new medications. ‘Green’ scCO 2 -based methodologies are well suited to improve either the synthesis or materials processing leading to the assembly of three-dimensional multifunctional constructs. By using scCO 2 either as C1 feedstock or as solvent, simple, economic, efficient and clean routes can be designed to synthesize materials with unique properties such as polyurea dendrimers and oxazoline-based polymers/oligomers. These new biocompatible, biodegradable and water-soluble polymeric materials can be engineered into multifunctional constructs with antimicrobial activity, targeting moieties, labelling units and/or efficiently loaded with therapeutics. This mini-review highlights the particular features exhibited by these materials resulting directly from the followed supercritical routes.

Novel Behavior in Smart Polymeric Materials: Stress Memory and its Potential Applications

2016 ◽  
Vol 97 ◽  
pp. 93-99
Author(s):  
Jin Lian Hu ◽  
Harishkumar Narayana
Keyword(s):  
Magnetic Field ◽  
Shape Memory ◽  
Smart Materials ◽  
Shape Memory Polymers ◽  
Polymeric Materials ◽  
External Stimulus ◽  
Artificial Muscles ◽  
Recovery Stress ◽  
Stress Memory ◽  
Potential Applications

Materials, structures and systems, responsive to an external stimulus are smart and adaptive to our human demands. Among smart materials, polymers with shape memory effect are at the forefront of research leading to comprehensive publications and wide applications. In this paper, we extend the concept of shape memory polymers to stress memory ones, which have been discovered recently. Like shape memory, stress memory represents a phenomenon where the stress in a polymer can be programmed, stored and retrieved reversibly with an external stimulus such as temperature and magnetic field. Stress memory may be mistaken as the recovery stress which was studied quite broadly. Our further investigation also reveals that stress memory is quite different from recovery stress containing multi-components including elastic and viscoelastic forces in addition to possible memory stress. Stress memory could be used into applications such as sensors, pressure garments, massage devices, electronic skins and artificial muscles. The current revelation of stress memory potentials is emanated from an authentic application of memory fibres, films, and foams in the smart compression devices for the management of chronic and therapeutic disorders.

scholarly journals Label-free prediction of three-dimensional fluorescence images from transmitted light microscopy

2018 ◽  
Author(s):  
Chawin Ounkomol ◽  
Sharmishtaa Seshamani ◽  
Mary M. Maleckar ◽  
Forrest Collman ◽  
Gregory R. Johnson
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Living Cells ◽  
Transmitted Light ◽  
Integrated Systems ◽  
Label Free ◽  
Subcellular Structure ◽  
Modern Biology ◽  
Transmitted Light Microscopy ◽  
Fluorescence Images

Understanding living cells as integrated systems, a challenge central to modern biology, is complicated by limitations of available imaging methods. While fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, slow, and damaging to cells. Here, we present a label-free method for predicting 3D fluorescence directly from transmitted light images and demonstrate that it can be used to generate multi-structure, integrated images.

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