Complex Intracellular Messenger Pathways Regulate One Type of Neuronal α-Bungarotoxin-Resistant Nicotinic Acetylcholine Receptors Expressed in Insect Neurosecretory Cells (Dorsal Unpaired Median Neurons)

2001 ◽  
Vol 60 (1) ◽  
pp. 80-91 ◽  
Author(s):  
Raphaël Courjaret ◽  
Bruno Lapied
Keyword(s):  
Nicotinic Acetylcholine Receptors ◽  
Acetylcholine Receptors ◽  
Neurosecretory Cells ◽  
Nicotinic Acetylcholine ◽  
Dorsal Unpaired Median ◽  
Intracellular Messenger

Imidacloprid actions on insect neuronal acetylcholine receptors

1997 ◽  
Vol 200 (21) ◽  
pp. 2685-2692 ◽  
Author(s):  
S Buckingham ◽  
B Lapied ◽  
H Corronc ◽  
F Sattelle
Keyword(s):  
Nervous System ◽  
Nicotinic Acetylcholine Receptors ◽  
Acetylcholine Receptors ◽  
Periplaneta Americana ◽  
Muscarinic Acetylcholine Receptors ◽  
Nicotinic Acetylcholine ◽  
Neonicotinoid Insecticide ◽  
Dose Dependent ◽  
Dorsal Unpaired Median ◽  
Giant Interneurone

The neonicotinoid insecticide Imidacloprid acts at three pharmacologically distinct acetylcholine receptor (AChR) subtypes in the cockroach (Periplaneta americana) nervous system, but is ineffective on muscarinic receptors. Imidacloprid (3-100µmoll-1) induced dose-dependent depolarizations at cockroach cercal afferent/giant interneurone synapses. These responses were insensitive to 20µmoll-1 atropine but were completely blocked by the nicotinic antagonist mecamylamine (50µmoll-1). Similarly, Imidacloprid-induced depolarizations of cultured cockroach dorsal unpaired median (DUM) neurones dissociated from the same (terminal abdominal) ganglion were also completely blocked by 100µmoll-1 mecamylamine. However, two components of the response could be distinguished on the basis of their differential sensitivities to 0.1µmoll-1-bungarotoxin (-BTX), which selectively blocks AChRs with 'mixed' nicotinic/muscarinic pharmacology in this preparation. This indicates that Imidacloprid affects both AChRs sensitive to -BTX and -BTX-insensitive nicotinic acetylcholine receptors (nAChRs). Thus, in the cockroach, Imidacloprid activates -BTX-sensitive synaptic nAChRs in giant interneurones, -BTX-insensitive extrasynaptic nAChRs in DUM neurones, and a recently characterized DUM neurone 'mixed' AChR that is sensitive to both nicotinic and muscarinic ligands. Imidacloprid does not act on muscarinic acetylcholine receptors (mAChRs) present on DUM neurone cell bodies and at the cercal afferent/giant interneurone synapses. This study shows that Imidacloprid can act on pharmacologically diverse nAChR subtypes.

Nicotinic acetylcholine receptors in the patients with Parkinson's disease: 5IA-SPECT

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S586-S586 ◽  
Author(s):  
Kazuo Hashikawa ◽  
Hidefumi Yoshida ◽  
Nobukatsu Sawamoto ◽  
Shigetoshi Takaya ◽  
Chihiro Namiki ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Nicotinic Acetylcholine Receptors ◽  
Acetylcholine Receptors ◽  
Nicotinic Acetylcholine
Sign in / Sign up

Export Citation Format

Share Document