High Affinity Binding of [3H]Propionyl-[Met(O2)11]Substance P(7–11), a Tritiated Septide-Like Peptide, in Chinese Hamster Ovary Cells Expressing Human Neurokinin-1 Receptors and in Rat Submandibular Glands

1997 ◽  
Vol 52 (1) ◽  
pp. 120-127 ◽  
Author(s):  
Sandrine Sagan ◽  
Jean-Claude Beaujouan ◽  
Yvette Torrens ◽  
Monique Saffroy ◽  
Gérard Chassaing ◽  
...  
Keyword(s):  
Substance P ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
Ovary Cells ◽  
Submandibular Glands ◽  
High Affinity ◽  
Neurokinin 1

Thyrotrophin-dependent desensitization by Chinese hamster ovary cells that express the recombinant human thyrotrophin receptor

1993 ◽  
Vol 139 (3) ◽  
pp. 425-429 ◽  
Author(s):  
K. Haraguchi ◽  
X. Peng ◽  
M. Kaneshige ◽  
E. Anzai ◽  
T. Endo ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Binding Sites ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Specific Factor ◽  
Affinity Binding ◽  
Ovary Cells ◽  
Subsequent Response ◽  
Higher Doses ◽  
Homologous Desensitization

ABSTRACT To determine whether thyrotrophin (TSH)-induced desensitization requires a thyroid-specific factor(s), the human TSH (hTSH) receptor was expressed in Chinese hamster ovary cells. The first incubation of the cells with TSH decreased the subsequent response of adenosine 3′,5′-cyclic monophosphate to freshly added TSH in the second incubation. This homologous desensitization was observed as early as after 3 h of the first incubation. The lowest dose of TSH that elicited desensitization was 0·1 nmol/l. The desensitization was not overcome by adding higher doses of TSH in the second incubation. A 125I-labelled TSH-binding study revealed a decrease in the number of high-affinity binding sites but not in that of low-affinity binding sites. The data suggest that TSH-induced desensitization in hTSH receptor-transfected cells is caused, at least in part, by a decrease in the number of TSH receptors on the cell surface. The evidence demonstrates, contrary to an earlier report, that a thyroid-specific factor(s) is not required for hTSH receptor desensitization. Journal of Endocrinology (1993) 139, 425–429

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells
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