High-Affinity Binding of Thyrotropin to the Extracellular Domain of Its Receptor Transfected in Chinese Hamster Ovary Cells

Thyroid ◽  
1993 ◽  
Vol 3 (2) ◽  
pp. 129-133 ◽  
Author(s):  
YUFEI SHI ◽  
MINJING ZOU ◽  
RANJIT S. PARHAR ◽  
NADIR R. FARID
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Extracellular Domain ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
Ovary Cells ◽  
High Affinity

Thyrotrophin-dependent desensitization by Chinese hamster ovary cells that express the recombinant human thyrotrophin receptor

1993 ◽  
Vol 139 (3) ◽  
pp. 425-429 ◽  
Author(s):  
K. Haraguchi ◽  
X. Peng ◽  
M. Kaneshige ◽  
E. Anzai ◽  
T. Endo ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Binding Sites ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Specific Factor ◽  
Affinity Binding ◽  
Ovary Cells ◽  
Subsequent Response ◽  
Higher Doses ◽  
Homologous Desensitization

ABSTRACT To determine whether thyrotrophin (TSH)-induced desensitization requires a thyroid-specific factor(s), the human TSH (hTSH) receptor was expressed in Chinese hamster ovary cells. The first incubation of the cells with TSH decreased the subsequent response of adenosine 3′,5′-cyclic monophosphate to freshly added TSH in the second incubation. This homologous desensitization was observed as early as after 3 h of the first incubation. The lowest dose of TSH that elicited desensitization was 0·1 nmol/l. The desensitization was not overcome by adding higher doses of TSH in the second incubation. A 125I-labelled TSH-binding study revealed a decrease in the number of high-affinity binding sites but not in that of low-affinity binding sites. The data suggest that TSH-induced desensitization in hTSH receptor-transfected cells is caused, at least in part, by a decrease in the number of TSH receptors on the cell surface. The evidence demonstrates, contrary to an earlier report, that a thyroid-specific factor(s) is not required for hTSH receptor desensitization. Journal of Endocrinology (1993) 139, 425–429

Efficient production and purification of extracellular domain of human FGFR-Fc fusion proteins from Chinese hamster ovary cells

2014 ◽  
Vol 99 ◽  
pp. 50-57 ◽  
Author(s):  
Aleksandra Sokolowska-Wedzina ◽  
Aleksandra Borek ◽  
Julia Chudzian ◽  
Piotr Jakimowicz ◽  
Malgorzata Zakrzewska ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Fusion Proteins ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Extracellular Domain ◽  
Efficient Production ◽  
Ovary Cells

scholarly journals Interferon γ Receptor Extracellular Domain Expressed as IgG Fusion Protein in Chinese Hamster Ovary Cells

1995 ◽  
Vol 270 (8) ◽  
pp. 3958-3964 ◽  
Author(s):  
Michael Fountoulakis ◽  
Cecilia Mesa ◽  
Georg Schmid ◽  
Reiner Gentz ◽  
Michael Manneberg ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Extracellular Domain ◽  
Interferon Γ ◽  
Ovary Cells

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells

Review of the current methods of Chinese Hamster Ovary (CHO) cells cultivation for production of therapeutic protein

2020 ◽  
Vol 17 ◽  
Author(s):  
Shazid Md. Sharker ◽  
Md. Atiqur Rahman
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Mass Production ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Therapeutic Protein ◽  
Specific Productivity ◽  
Ovary Cells ◽  
Further Development ◽  
Interesting Area

Most of clinical approved protein-based drugs or under in clinical trial have a profound impact in the treatment of critical diseases. The mammalian eukaryotic cells culture approaches, particularly the CHO (Chinese Hamster Ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of therapeutic protein. Recent advances in CHO cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. The developments of cell lines are possible to upgrade specific productivity. As a result, it holds an interesting area for academic as well as industrial researchers around the world. This review will concentrate on the recent progress of the mammalian CHO cells culture technology and the future scope of further development for the mass-production of protein therapeutics.

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