scholarly journals TCL1A Single-Nucleotide Polymorphisms and Estrogen-Mediated Toll-Like Receptor-MYD88–Dependent Nuclear Factor-κB Activation: Single-Nucleotide Polymorphism– and Selective Estrogen Receptor Modulator–Dependent Modification of Inflammation and Immune Response

2017 ◽  
Vol 92 (2) ◽  
pp. 175-184 ◽  
Author(s):  
Ming-Fen Ho ◽  
James N. Ingle ◽  
Tim Bongartz ◽  
Krishna R. Kalari ◽  
Paul E. Goss ◽  
...  
Keyword(s):  
Immune Response ◽  
Single Nucleotide Polymorphism ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Nucleotide Polymorphisms ◽  
Toll Like Receptor ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Receptor Modulator ◽  
Dependent Modification

A toll-like receptor 3 single nucleotide polymorphism in Japanese patients with sarcoidosis

2015 ◽  
Vol 85 (3) ◽  
pp. 204-208 ◽  
Author(s):  
K. Ikezoe ◽  
T. Handa ◽  
K. Tanizawa ◽  
T. Kubo ◽  
I. Ito ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Japanese Patients ◽  
Toll Like Receptor ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide

scholarly journals Toll-like Receptor 1 N248S Single-Nucleotide Polymorphism Is Associated With Leprosy Risk and Regulates Immune Activation During Mycobacterial Infection

2013 ◽  
Vol 208 (1) ◽  
pp. 120-129 ◽  
Author(s):  
Carolinne de Sales Marques ◽  
Vânia N. Brito-de-Souza ◽  
Luana Tatiana Albuquerque Guerreiro ◽  
João H. Martins ◽  
Evaldo P. Amaral ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Immune Activation ◽  
Mycobacterial Infection ◽  
Toll Like Receptor ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide

scholarly journals Single-Nucleotide-Polymorphism-Specific PCR for Quantification and Discrimination of Chlamydia pneumoniae Genotypes by Use of a “Locked” Nucleic Acid

2006 ◽  
Vol 72 (5) ◽  
pp. 3785-3787 ◽  
Author(s):  
Jan Rupp ◽  
Werner Solbach ◽  
Jens Gieffers
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Chlamydia Pneumoniae ◽  
Locked Nucleic Acid ◽  
Nucleotide Polymorphisms ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Specific Pcr ◽  
Intraspecies Diversity

ABSTRACT Single-nucleotide polymorphisms (SNPs) are targets to discriminate intraspecies diversity of bacteria and to correlate a genotype with a potential pathotype. Quantification of polygenotypic populations supports this task for in vitro and in vivo applications. We present a novel assay capable of quantifying mixtures of two genotypes differing by only one SNP.

scholarly journals Toll-Like Receptor-1 Single-Nucleotide Polymorphism 1805T/G Is Associated With Predisposition to Multibacillary Tuberculosis

2018 ◽  
Vol 9 ◽  
Author(s):  
Raphaela Honorato Barletta-Naveca ◽  
Felipe Gomes Naveca ◽  
Vanessa Alves de Almeida ◽  
Jorge Ivan Rebelo Porto ◽  
George Allan Villarouco da Silva ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Toll Like Receptor ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide

scholarly journals Quantitative Analysis of Single-Nucleotide Polymorphism for Rapid Detection of TR34/L98H- and TR46/Y121F/T289A-Positive Aspergillus fumigatus Isolates Obtained from Patients in Iran from 2010 to 2014

2015 ◽  
Vol 60 (1) ◽  
pp. 387-392 ◽  
Author(s):  
Faezeh Mohammadi ◽  
Seyed Jamal Hashemi ◽  
Jan Zoll ◽  
Willem J. G. Melchers ◽  
Haleh Rafati ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Quantitative Analysis ◽  
Single Nucleotide Polymorphisms ◽  
Aspergillus Fumigatus ◽  
Tandem Repeat ◽  
Promoter Region ◽  
Nucleotide Polymorphisms ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Content Type

ABSTRACTWe employed an endpoint genotyping method to update the prevalence rate of positivity for the TR34/L98H mutation (a 34-bp tandem repeat mutation in the promoter region of thecyp51Agene in combination with a substitution at codon L98) and the TR46/Y121F/T289A mutation (a 46-bp tandem repeat mutation in the promoter region of thecyp51Agene in combination with substitutions at codons Y121 and T289) among clinicalAspergillus fumigatusisolates obtained from different regions of Iran over a recent 5-year period (2010 to 2014). The antifungal activities of itraconazole, voriconazole, and posaconazole against 172 clinicalA. fumigatusisolates were investigated using the European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth microdilution method. For the isolates with an azole resistance phenotype, thecyp51Agene and its promoter were amplified and sequenced. In addition, using a LightCycler 480 real-time PCR system, a novel endpoint genotyping analysis method targeting single-nucleotide polymorphisms was evaluated to detect the L98H and Y121F mutations in thecyp51Agene of all isolates. Of the 172A. fumigatusisolates tested, the MIC values of itraconazole (≥16 mg/liter) and voriconazole (>4 mg/liter) were high for 6 (3.5%). Quantitative analysis of single-nucleotide polymorphisms showed the TR34/L98H mutation in thecyp51Agenes of six isolates. No isolates harboring the TR46/Y121F/T289A mutation were detected. DNA sequencing of thecyp51Agene confirmed the results of the novel endpoint genotyping method. By microsatellite typing, all of the azole-resistant isolates had genotypes different from those previously recovered from Iran and from the Dutch TR34/L98H controls. In conclusion, there was not a significant increase in the prevalence of azole-resistantA. fumigatusisolates harboring the TR34/L98H resistance mechanism among isolates recovered over a recent 5-year period (2010 to 2014) in Iran. A quantitative assay detecting a single-nucleotide polymorphism in thecyp51Agene ofA. fumigatusis a reliable tool for the rapid screening and monitoring of TR34/L98H- and TR46/Y121F/T289A-positive isolates and can easily be incorporated into clinical mycology algorithms.

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