scholarly journals Subtype-Specific Mechanisms for Functional Interaction between α6β4* Nicotinic Acetylcholine Receptors and P2X Receptors

2014 ◽  
Vol 86 (3) ◽  
pp. 263-274 ◽  
Author(s):  
Walrati Limapichat ◽  
Dennis A. Dougherty ◽  
Henry A. Lester
Keyword(s):  
Nicotinic Acetylcholine Receptors ◽  
Acetylcholine Receptors ◽  
P2x Receptors ◽  
Functional Interaction ◽  
Nicotinic Acetylcholine

scholarly journals Functional interaction between Lypd6 and nicotinic acetylcholine receptors

2016 ◽  
Vol 138 (6) ◽  
pp. 806-820 ◽  
Author(s):  
Maria Arvaniti ◽  
Majbrit M. Jensen ◽  
Neeraj Soni ◽  
Hong Wang ◽  
Anders B. Klein ◽  
...  
Keyword(s):  
Nicotinic Acetylcholine Receptors ◽  
Acetylcholine Receptors ◽  
Functional Interaction ◽  
Nicotinic Acetylcholine

scholarly journals Cross-inhibition between nicotinic acetylcholine receptors and P2X receptors in myenteric neurons and HEK-293 cells

2009 ◽  
Vol 296 (6) ◽  
pp. G1267-G1276 ◽  
Author(s):  
Dima A. Decker ◽  
James J. Galligan
Keyword(s):  
Nicotinic Acetylcholine Receptors ◽  
Acetylcholine Receptors ◽  
P2x Receptors ◽  
P2x Receptor ◽  
Nicotinic Acetylcholine ◽  
Hek 293 ◽  
Myenteric Neurons ◽  
Hek 293 Cells ◽  
293 Cells ◽  
Cross Inhibition

The enteric nervous system (ENS) controls gut function. P2X receptors and nicotinic acetylcholine receptors (nAChRs) are ligand-gated cation channels that mediate fast synaptic excitation in the ENS. Close molecular coupling in enteric neuronal membranes contributes to a mutually inhibitory interaction between these receptors; this effect is called cross-inhibition. We studied the molecular mechanisms responsible for cross-inhibition. Whole cell patch-clamp techniques were used to measure P2X- and nAChR-mediated currents in cultured enteric neurons and HEK-293 cells. In cultured myenteric neurons, ACh (3 mM) and ATP (1 mM) coapplication evoked an inward current that was only 57 ± 6% ( P < 0.05) of the predicted current that would have occurred if the two populations of channels were activated independently. In HEK-293 cells coexpressing α3β4 nAChR/P2X2 receptors, coapplication of ATP and ACh caused a current that was 58 ± 7% of the predicted current ( P < 0.05). To test the importance of P2X subunit COOH-terminal tail length on cross-inhibition, P2X3 and P2X4 subunits, which have shorter COOH-terminal tails, were studied. Cross-inhibition with α3β4 nAChRs and P2X3 or P2X4 subunits was similar to that occurring with P2X2 subunits. P2X receptor or α3β4 nAChR desensitization did not prevent receptor cross-inhibition. These data indicate that the α3β4-P2X receptor interaction is not restricted to P2X2 subunits. In addition, active and desensitized conformations of the P2X receptor inhibit nAChR function. These molecular interactions may modulate the function of synapses that use ATP and ACh as fast synaptic transmitters in the ENS.

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