scholarly journals Resveratrol Sensitizes Acute Myelogenous Leukemia Cells to Histone Deacetylase Inhibitors through Reactive Oxygen Species-Mediated Activation of the Extrinsic Apoptotic Pathway

2012 ◽  
Vol 82 (6) ◽  
pp. 1030-1041 ◽  
Author(s):  
Alae Yaseen ◽  
Shuang Chen ◽  
Stefanie Hock ◽  
Roberto Rosato ◽  
Paul Dent ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Histone Deacetylase ◽  
Acute Myelogenous Leukemia ◽  
Histone Deacetylase Inhibitors ◽  
Reactive Oxygen ◽  
Myelogenous Leukemia ◽  
Leukemia Cells ◽  
Oxygen Species ◽  
Apoptotic Pathway ◽  
Acute Myelogenous

scholarly journals Generation of Reactive Oxygen Species during Apoptosis Induced by DNA-Damaging Agents and/or Histone Deacetylase Inhibitors

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Barbora Brodská ◽  
Aleš Holoubek
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Line ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitors ◽  
Actinomycin D ◽  
Reactive Oxygen ◽  
Leukemia Cell Line ◽  
Ros Production ◽  
Oxygen Species ◽  
Dna Damaging Agents

Reactive oxygen species play an important role in the process of apoptosis in many cell types. In this paper, we analyzed the role of ROS in DNA-damaging agents (actinomycin D or decitabine), which induced apoptosis of leukemia cell line CML-T1 and normal peripheral blood lymphocytes (PBL). The possibility of synergism with histone deacetylase inhibitors butyrate or SAHA is also reported. We found that in cancer cell line, ROS production significantly contributed to apoptosis triggering, while in normal lymphocytes treated by cytostatic or cytotoxic drugs, necrosis as well as apoptosis occurred and large heterogeneity of ROS production was measured. Combined treatment with histone deacetylase inhibitor did not potentiate actinomycin D action, whereas combination of decitabine and SAHA brought synergistic ROS generation and apoptotic features in CML cell line. Appropriate decrease of cell viability indicated promising therapeutic potential of this combination in CML, but side effects on normal PBL should be taken into attention.

Sign in / Sign up

Export Citation Format

Share Document