scholarly journals Generation of Reactive Oxygen Species during Apoptosis Induced by DNA-Damaging Agents and/or Histone Deacetylase Inhibitors

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Barbora Brodská ◽  
Aleš Holoubek
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Line ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitors ◽  
Actinomycin D ◽  
Reactive Oxygen ◽  
Leukemia Cell Line ◽  
Ros Production ◽  
Oxygen Species ◽  
Dna Damaging Agents

Reactive oxygen species play an important role in the process of apoptosis in many cell types. In this paper, we analyzed the role of ROS in DNA-damaging agents (actinomycin D or decitabine), which induced apoptosis of leukemia cell line CML-T1 and normal peripheral blood lymphocytes (PBL). The possibility of synergism with histone deacetylase inhibitors butyrate or SAHA is also reported. We found that in cancer cell line, ROS production significantly contributed to apoptosis triggering, while in normal lymphocytes treated by cytostatic or cytotoxic drugs, necrosis as well as apoptosis occurred and large heterogeneity of ROS production was measured. Combined treatment with histone deacetylase inhibitor did not potentiate actinomycin D action, whereas combination of decitabine and SAHA brought synergistic ROS generation and apoptotic features in CML cell line. Appropriate decrease of cell viability indicated promising therapeutic potential of this combination in CML, but side effects on normal PBL should be taken into attention.

scholarly journals GATA‐1 isoforms differently contribute to the production and compartmentation of reactive oxygen species in the myeloid leukemia cell line K562

2019 ◽  
Vol 234 (11) ◽  
pp. 20829-20846
Author(s):  
Patrizia Riccio ◽  
Raffaele Sessa ◽  
Sergio Nicola ◽  
Fara Petruzziello ◽  
Silvia Trombetti ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Line ◽  
Myeloid Leukemia ◽  
Leukemia Cell ◽  
Reactive Oxygen ◽  
Leukemia Cell Line ◽  
Oxygen Species ◽  
Myeloid Leukemia Cell

scholarly journals Cyclic mechanical strain increases reactive oxygen species production in pulmonary epithelial cells

2005 ◽  
Vol 289 (5) ◽  
pp. L834-L841 ◽  
Author(s):  
Kenneth E. Chapman ◽  
Scott E. Sinclair ◽  
Daming Zhuang ◽  
Aviv Hassid ◽  
Leena P. Desai ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Epithelial Cells ◽  
Cell Line ◽  
Primary Cultures ◽  
Reactive Oxygen ◽  
Lung Epithelial Cells ◽  
Alveolar Type ◽  
Ros Production ◽  
Oxygen Species ◽  
Type Ii

Overdistention of lung tissue during mechanical ventilation may be one of the factors that initiates ventilator-induced lung injury (VILI). We hypothesized that cyclic mechanical stretch (CMS) of the lung epithelium is involved in the early events of VILI through the production of reactive oxygen species (ROS). Cultures of an immortalized human airway epithelial cell line (16HBE), a human alveolar type II cell line (A549), and primary cultures of rat alveolar type II cells were cyclically stretched, and the production of superoxide (O2−) was measured by dihydroethidium fluorescence. CMS stimulated increased production of O2− after 2 h in each type of cell. 16HBE cells exhibited no significant stimulation of ROS before 2 h of CMS (20% strain, 30 cycles/min), and ROS production returned to control levels after 24 h. Oxidation of glutathione (GSH), a cellular antioxidant, increased with CMS as measured by a decrease in the ratio of the reduced GSH level to the oxidized GSH level. Strain levels of 10% did not increase O2− production in 16HBE cells, whereas 15, 20, and 30% significantly increased generation of O2−. Rotenone, a mitochondrial complex I inhibitor, partially abrogated the stretch-induced generation of O2− after 2 h CMS in 16HBE cells. NADPH oxidase activity was increased after 2 h of CMS, contributing to the production of O2−. Increased ROS production in lung epithelial cells in response to elevated stretch may contribute to the onset of VILI.

Fetal hemoglobin induction by histone deacetylase inhibitors involves generation of reactive oxygen species

2006 ◽  
Vol 34 (3) ◽  
pp. 264-273 ◽  
Author(s):  
Cheng-Hui Hsiao ◽  
Wei Li ◽  
Tzu-Fang Lou ◽  
B. Surendra Baliga ◽  
Betty S. Pace
Keyword(s):  
Reactive Oxygen Species ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitors ◽  
Reactive Oxygen ◽  
Fetal Hemoglobin ◽  
Oxygen Species
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