Therapeutic Potential of Nitric Oxide-Modified Drugs in Colon Cancer Cells

2012 ◽  
Vol 82 (4) ◽  
pp. 700-710 ◽  
Author(s):  
Marija Mojic ◽  
Sanja Mijatovic ◽  
Danijela Maksimovic-Ivanic ◽  
Djordje Miljkovic ◽  
Stanislava Stosic-Grujicic ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Therapeutic Potential ◽  
Colon Cancer Cells

Cytotoxic Effect of the Combination of Gemcitabine and Atorvastatin Loaded in Microemulsion on the HCT116 Colon Cancer Cells

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Mayson H. Alkhatib ◽  
Dalal Al-Saedi ◽  
Wadiah S. Backer
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Anticancer Drugs ◽  
Therapeutic Potential ◽  
Morphological Changes ◽  
In Vitro Study ◽  
Colon Cancer Cells ◽  
Apoptosis Detection ◽  
Hct116 Cells

The combination of anticancer drugs in nanoparticles has great potential as a promising strategy to maximize efficacies by eradicating resistant, reduce the dosage of the drug and minimize toxicities on the normal cells. Gemcitabine (GEM), a nucleoside analogue, and atorvastatin (ATV), a cholesterol lowering agent, have shown anticancer effect with some limitations. The objective of this in vitro study was to evaluate the antitumor activity of the combination therapy of GEM and ATVencapsulated in a microemulsion (ME) formulation in the HCT116 colon cancer cells. The cytotoxicity and efficacy of the formulation were assessed by the 3- (4,5dimethylthiazole-2-yl)-2,5-diphyneltetrazolium bromide (MTT) assay. The mechanism of cell death was examined by observing the morphological changes of treated cells under light microscope, identifying apoptosis by using the ApopNexin apoptosis detection kit, and viewing the morphological changes in the chromatin structure stained with 4′,6-diamidino-2-phenylindole (DAPI) under the inverted fluorescence microscope. It has been found that reducing the concentration of GEM loaded on ME (GEM-ME) from 5μM to 1.67μM by combining it with 3.33μM of ATV in a ME formulation (GEM/2ATV-ME) has preserved the strong cytotoxicity of GEM-ME against HCT116 cells. The current study proved that formulating GEM with ATV in ME has improved the therapeutic potential of GEM and ATV as anticancer drugs.

scholarly journals Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α

Cancers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 113 ◽  
Author(s):  
Alaa A. A. Aljabali ◽  
Hamid A. Bakshi ◽  
Faruck L. Hakkim ◽  
Yusuf A. Haggag ◽  
Khalid M. Al-Batanyeh ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Anticancer Agents ◽  
Therapeutic Potential ◽  
Hypoxia Inducible Factor ◽  
In Vitro Cytotoxicity ◽  
Colon Cancer Cells ◽  
Chemical Colitis ◽  
Clinical Trails

Piceatannol (PIC) is known to have anticancer activity, which has been attributed to its ability to block the proliferation of cancer cells via suppression of the NF-kB signaling pathway. However, its effect on hypoxia-inducible factor (HIF) is not well known in cancer. In this study, PIC was loaded into bovine serum albumin (BSA) by desolvation method as PIC–BSA nanoparticles (NPs). These PIC–BSA nanoparticles were assessed for in vitro cytotoxicity, migration, invasion, and colony formation studies and levels of p65 and HIF-1α. Our results indicate that PIC–BSA NPs were more effective in downregulating the expression of nuclear p65 and HIF-1α in colon cancer cells as compared to free PIC. We also observed a significant reduction in inflammation induced by chemical colitis in mice by PIC–BSA NPs. Furthermore, a significant reduction in tumor size and number of colon tumors was also observed in the murine model of colitis-associated colorectal cancer, when treated with PIC–BSA NPs as compared to free PIC. The overall results indicate that PIC, when formulated as PIC–BSA NPs, enhances its therapeutic potential. Our work could prompt further research in using natural anticancer agents as nanoparticels with possible human clinical trails. This could lead to the development of a new line of safe and effective therapeutics for cancer patients.

Influence of the nitric oxide donor glyceryl trinitrate on apoptotic pathways in human colon cancer cells

2002 ◽  
Vol 123 (1) ◽  
pp. 235-246 ◽  
Author(s):  
Anne Millet ◽  
Ali Bettaieb ◽  
Flore Renaud ◽  
Laurent Prevotat ◽  
Arlette Hammann ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Colon Cancer ◽  
Glyceryl Trinitrate ◽  
Cancer Cells ◽  
Human Colon ◽  
Nitric Oxide Donor ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Apoptotic Pathways ◽  
Human Colon Cancer Cells

QS36. Nitric Oxide-Mediated Radiosensitization to Ionizing Radiation-Induced Apoptosis of Colon Cancer Cells

2008 ◽  
Vol 144 (2) ◽  
pp. 283-284
Author(s):  
Derrick Chen ◽  
Laura Ortega ◽  
David Chen ◽  
Edward H. Livingston ◽  
Sergio Huerta
Keyword(s):  
Nitric Oxide ◽  
Colon Cancer ◽  
Ionizing Radiation ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Radiation Induced ◽  
Induced Apoptosis
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