Therapeutic potential of a cell penetrating peptide (CPP, NP1) mediated siRNA delivery: Evidence in 3D spheroids of colon cancer cells

2020 ◽  
Vol 98 (6) ◽  
pp. 1240-1254 ◽  
Author(s):  
Khalsa Al‐Husaini ◽  
Erij Elkamel ◽  
Xiaoxia Han ◽  
Pu Chen
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Therapeutic Potential ◽  
Sirna Delivery ◽  
Cell Penetrating Peptide ◽  
Colon Cancer Cells ◽  
Cell Penetrating ◽  
A Cell ◽  
3D Spheroids

scholarly journals Blocking hepatic metastases of colon cancer cells using an shRNA against Rac1 delivered by activatable cell-penetrating peptide

Oncotarget ◽  
2016 ◽  
Vol 7 (47) ◽  
pp. 77183-77195 ◽  
Author(s):  
Ying Bao ◽  
Huihui Guo ◽  
Yongliang Lu ◽  
Wenming Feng ◽  
Xinrong Sun ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Hepatic Metastases ◽  
Cell Penetrating Peptide ◽  
Colon Cancer Cells ◽  
Cell Penetrating

Cytotoxic Effect of the Combination of Gemcitabine and Atorvastatin Loaded in Microemulsion on the HCT116 Colon Cancer Cells

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Mayson H. Alkhatib ◽  
Dalal Al-Saedi ◽  
Wadiah S. Backer
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Anticancer Drugs ◽  
Therapeutic Potential ◽  
Morphological Changes ◽  
In Vitro Study ◽  
Colon Cancer Cells ◽  
Apoptosis Detection ◽  
Hct116 Cells

The combination of anticancer drugs in nanoparticles has great potential as a promising strategy to maximize efficacies by eradicating resistant, reduce the dosage of the drug and minimize toxicities on the normal cells. Gemcitabine (GEM), a nucleoside analogue, and atorvastatin (ATV), a cholesterol lowering agent, have shown anticancer effect with some limitations. The objective of this in vitro study was to evaluate the antitumor activity of the combination therapy of GEM and ATVencapsulated in a microemulsion (ME) formulation in the HCT116 colon cancer cells. The cytotoxicity and efficacy of the formulation were assessed by the 3- (4,5dimethylthiazole-2-yl)-2,5-diphyneltetrazolium bromide (MTT) assay. The mechanism of cell death was examined by observing the morphological changes of treated cells under light microscope, identifying apoptosis by using the ApopNexin apoptosis detection kit, and viewing the morphological changes in the chromatin structure stained with 4′,6-diamidino-2-phenylindole (DAPI) under the inverted fluorescence microscope. It has been found that reducing the concentration of GEM loaded on ME (GEM-ME) from 5μM to 1.67μM by combining it with 3.33μM of ATV in a ME formulation (GEM/2ATV-ME) has preserved the strong cytotoxicity of GEM-ME against HCT116 cells. The current study proved that formulating GEM with ATV in ME has improved the therapeutic potential of GEM and ATV as anticancer drugs.

scholarly journals Establishment and Characterization of a Cell Line from Human Circulating Colon Cancer Cells

2015 ◽  
Vol 75 (5) ◽  
pp. 892-901 ◽  
Author(s):  
Laure Cayrefourcq ◽  
Thibault Mazard ◽  
Simon Joosse ◽  
Jérôme Solassol ◽  
Jeanne Ramos ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Colon Cancer Cells ◽  
A Cell

scholarly journals Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer Via Downregulation of Nuclear p65 and HIF-1α

Cancers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 113 ◽  
Author(s):  
Alaa A. A. Aljabali ◽  
Hamid A. Bakshi ◽  
Faruck L. Hakkim ◽  
Yusuf A. Haggag ◽  
Khalid M. Al-Batanyeh ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Anticancer Agents ◽  
Therapeutic Potential ◽  
Hypoxia Inducible Factor ◽  
In Vitro Cytotoxicity ◽  
Colon Cancer Cells ◽  
Chemical Colitis ◽  
Clinical Trails

Piceatannol (PIC) is known to have anticancer activity, which has been attributed to its ability to block the proliferation of cancer cells via suppression of the NF-kB signaling pathway. However, its effect on hypoxia-inducible factor (HIF) is not well known in cancer. In this study, PIC was loaded into bovine serum albumin (BSA) by desolvation method as PIC–BSA nanoparticles (NPs). These PIC–BSA nanoparticles were assessed for in vitro cytotoxicity, migration, invasion, and colony formation studies and levels of p65 and HIF-1α. Our results indicate that PIC–BSA NPs were more effective in downregulating the expression of nuclear p65 and HIF-1α in colon cancer cells as compared to free PIC. We also observed a significant reduction in inflammation induced by chemical colitis in mice by PIC–BSA NPs. Furthermore, a significant reduction in tumor size and number of colon tumors was also observed in the murine model of colitis-associated colorectal cancer, when treated with PIC–BSA NPs as compared to free PIC. The overall results indicate that PIC, when formulated as PIC–BSA NPs, enhances its therapeutic potential. Our work could prompt further research in using natural anticancer agents as nanoparticels with possible human clinical trails. This could lead to the development of a new line of safe and effective therapeutics for cancer patients.

Therapeutic Potential of Nitric Oxide-Modified Drugs in Colon Cancer Cells

2012 ◽  
Vol 82 (4) ◽  
pp. 700-710 ◽  
Author(s):  
Marija Mojic ◽  
Sanja Mijatovic ◽  
Danijela Maksimovic-Ivanic ◽  
Djordje Miljkovic ◽  
Stanislava Stosic-Grujicic ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Therapeutic Potential ◽  
Colon Cancer Cells

A Cell-Penetrating Peptide Targeting AAC-11 Specifically Induces Cancer Cells Death

2016 ◽  
Vol 76 (18) ◽  
pp. 5479-5490 ◽  
Author(s):  
Léonard Jagot-Lacoussiere ◽  
Ewa Kotula ◽  
Bruno O. Villoutreix ◽  
Heriberto Bruzzoni-Giovanelli ◽  
Jean-Luc Poyet
Keyword(s):  
Cancer Cells ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
A Cell ◽  
Peptide Targeting ◽  
Cells Death

A novel apoptosis-inducing monoclonal antibody (anti-LHK) against a cell surface antigen on colon cancer cells

2005 ◽  
Vol 40 (10) ◽  
pp. 945-955 ◽  
Author(s):  
Hidehiko Matsukawa ◽  
Takanori Kanai ◽  
Makoto Naganuma ◽  
Nobuhiko Kamada ◽  
Tadakazu Hisamatsu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Colon Cancer ◽  
Cell Surface ◽  
Cancer Cells ◽  
Surface Antigen ◽  
Cell Surface Antigen ◽  
Colon Cancer Cells ◽  
A Cell

Melatonin Enhances Anti-tumoral Effects of Menadione on Colon Cancer Cells

2021 ◽  
Vol 21 ◽  
Author(s):  
Alejandro Collin ◽  
Romina Kohan ◽  
Nori Tolosa de Talamoni ◽  
Gabriela Picotto
Keyword(s):  
Colon Cancer ◽  
Cell Migration ◽  
Cancer Cells ◽  
Nitrosative Stress ◽  
Therapeutic Potential ◽  
Cellular Growth ◽  
Dependent Manner ◽  
Colon Cancer Cells ◽  
Cancer Cell Growth ◽  
Oxidative And Nitrosative Stress

Background: Colon cancer is one of the most important causes of death in the entire world. New pharmacological strategies are always needed, especially in resistant variants of this pathology. We have previously reported that drugs such as menadione (MEN), D, L-buthionine-S,R-sulfoximine or calcitriol, used in combination, enhanced cell sensibility of breast and colon tumour models, due to their ability to modify the oxidative status of the cells. Melatonin (MEL), a hormone regulating circadian rhythms, has anti-oxidant and anti-apoptotic properties at low concentrations, while at high doses, it has been shown to inhibit cancer cell growth. Objective: The objective of this study is to determine the antitumoral action of the combination MEN and MEL on colon cancer cells. Methods: Caco-2 cells were employed to evaluate the effects of both compounds, used alone or combined, on cellular growth/morphology, oxidative and nitrosative stress, and cell migration. Results: MEN plus MEL dramatically reduced cell proliferation in a time and dose-dependent manner. The antiproliferative effects began at 48 h. At the same time, the combination modified the content of superoxide anion, induced the formation of reactive nitrogen species and enhanced catalase activity. Cell migration process was delayed. Also, changes in nuclear morphology consistent with cell death were observed. Conclusion: The enhanced effect of simultaneous use of MEN and MEL on Caco-2 cells suggests that this combined action may have therapeutic potential as an adjuvant on intestinal cancer acting in different oncogenic pathways.

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