scholarly journals Regional Proteomic Quantification of Clinically Relevant Non-Cytochrome P450 Enzymes along the Human Small Intestine

2020 ◽  
Vol 48 (7) ◽  
pp. 528-536 ◽  
Author(s):  
Haeyoung Zhang ◽  
Chris Wolford ◽  
Abdul Basit ◽  
Albert P. Li ◽  
Peter W. Fan ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Small Intestine ◽  
Cytochrome P450 Enzymes ◽  
Human Small Intestine

scholarly journals Application of Caco-2 Cell Line in Herb-Drug Interaction Studies: Current Approaches and Challenges

2014 ◽  
Vol 17 (1) ◽  
pp. 1 ◽  
Author(s):  
Charles Awortwe ◽  
P.S. Fasinu ◽  
B. Rosenkranz
Keyword(s):  
Small Intestine ◽  
Drug Interactions ◽  
Cell Line ◽  
Cytochrome P450 Enzymes ◽  
Human Small Intestine ◽  
Clinical Investigations ◽  
New Chemical Entities ◽  
Biopharmaceutics Classification ◽  
Gastrointestinal Permeability ◽  
Laboratory Protocol

The Caco-2 model is employed in pre-clinical investigations to predict the likely gastrointestinal permeability of drugs because it expresses cytochrome P450 enzymes, transporters, microvilli and enterocytes of identical characteristics to the human small intestine. The FDA recommends this model as integral component of the Biopharmaceutics Classification System (BCS). Most dedicated laboratories use the Caco-2 cell line to screen new chemical entities through prediction of its solubility, bioavailability and the possibility of drug-drug or herb-drug interactions in the gut lumen. However, challenges in the inherent characteristics of Caco-2 cell and inter-laboratory protocol variations have resulted to generation of irreproducible data. These limitations affect the extrapolation of data from pre-clinical research to clinical studies involving drug-drug and herb-drug interactions. This review addresses some of these caveats and enumerates the plausible current and future approaches to reduce the anomalies associated with Caco-2 cell line investigations focusing on its application in herb-drug interactions. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Disruption of cytochrome P450 enzymes in the liver and small intestine in chicken embryos in ovo exposed to glyphosate

2020 ◽  
Vol 27 (14) ◽  
pp. 16865-16875 ◽  
Author(s):  
Mohamed Ahmed Fathi ◽  
Guofeng Han ◽  
Ruifen Kang ◽  
Dan Shen ◽  
Jiakun Shen ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Small Intestine ◽  
Cytochrome P450 Enzymes ◽  
In Ovo ◽  
Chicken Embryos

scholarly journals Immunochemical detection of cytochrome P450 enzymes in small intestine microsomes of male and female untreated juvenile cynomolgus monkeys

Xenobiotica ◽  
2014 ◽  
Vol 44 (9) ◽  
pp. 769-774 ◽  
Author(s):  
Shotaro Uehara ◽  
Norie Murayama ◽  
Yasuharu Nakanishi ◽  
Chika Nakamura ◽  
Takanori Hashizume ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Small Intestine ◽  
Cytochrome P450 Enzymes ◽  
Male And Female ◽  
Cynomolgus Monkeys ◽  
Immunochemical Detection

cDNA Cloning and Expression of a Novel Cytochrome P450 (CYP4F12) from Human Small Intestine

2001 ◽  
Vol 280 (4) ◽  
pp. 1135-1141 ◽  
Author(s):  
Takanori Hashizume ◽  
Susumu Imaoka ◽  
Toyoko Hiroi ◽  
Yoshiaki Terauchi ◽  
Toshihiko Fujii ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Small Intestine ◽  
Cdna Cloning ◽  
Cloning And Expression ◽  
Human Small Intestine

Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

1986 ◽  
Vol 44 ◽  
pp. 134-135
Author(s):  
A. J. Tousimis
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Elemental Composition ◽  
Isotope Labeling ◽  
Structural Components ◽  
X Ray ◽  
Human Small Intestine ◽  
Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

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