In vivo gene expression profile of human intestinal epithelial cells: from the viewpoint of drug metabolism and pharmacokinetics

2020 ◽  
pp. DMD-AR-2020-000283
Author(s):  
Kazuo Takayama ◽  
Kohei Ito ◽  
Akiko Matsui ◽  
Tomoki Yamashita ◽  
Kentaro Kawakami ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Drug Metabolism ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

Influence of TNF-α on the gene expression profile in src transtormed intestinal epithelial cells

2001 ◽  
Vol 120 (5) ◽  
pp. A300 ◽  
Author(s):  
Naoki Kawai ◽  
Shingo Tsuji ◽  
Masahiko Tsujii ◽  
Masato Komori ◽  
Arata Kimura ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Tnf Α

Influence of TNF-α on the gene expression profile in src transtormed intestinal epithelial cells

2001 ◽  
Vol 120 (5) ◽  
pp. A300-A300
Author(s):  
N KAWAI ◽  
S TSUJI ◽  
M TSUJII ◽  
M KOMORI ◽  
A KIMURA ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Tnf Α

Comparison of gene expression and activation of transcription factors in organoid-derived monolayer intestinal epithelial cells and organoids

2021 ◽  
Author(s):  
Yu Takahashi ◽  
Yu Inoue ◽  
Keitaro Kuze ◽  
Shintaro Sato ◽  
Makoto Shimizu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Regulation ◽  
Intestinal Epithelial Cells ◽  
Three Dimensional ◽  
Culture Conditions ◽  
Dependent Manner ◽  
Intestinal Epithelial

Abstract Intestinal organoids better represent in vivo intestinal properties than conventionally used established cell lines in vitro. However, they are maintained in three-dimensional culture conditions that may be accompanied by handling complexities. We characterized the properties of human organoid-derived two-dimensionally cultured intestinal epithelial cells (IECs) compared with those of their parental organoids. We found that the expression of several intestinal markers and functional genes were indistinguishable between monolayer IECs and organoids. We further confirmed that their specific ligands equally activate intestinal ligand-activated transcriptional regulators in a dose-dependent manner. The results suggest that culture conditions do not significantly influence the fundamental properties of monolayer IECs originating from organoids, at least from the perspective of gene expression regulation. This will enable their use as novel biological tools to investigate the physiological functions of the human intestine.

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