scholarly journals Regulation of Sucrase-Isomaltase Gene Expression in Human Intestinal Epithelial Cells by Inflammatory Cytokines

1996 ◽  
Vol 271 (2) ◽  
pp. 1237-1242 ◽  
Author(s):  
Theodoros Ziambaras ◽  
Deborah C. Rubin ◽  
David H. Perlmutter
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Inflammatory Cytokines ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Human Intestinal Epithelial Cells

scholarly journals Interferon Regulatory Factor 1 (IRF-1) and IRF-2 Distinctively Up-Regulate Gene Expression and Production of Interleukin-7 in Human Intestinal Epithelial Cells

2004 ◽  
Vol 24 (14) ◽  
pp. 6298-6310 ◽  
Author(s):  
Shigeru Oshima ◽  
Tetsuya Nakamura ◽  
Shin Namiki ◽  
Eriko Okada ◽  
Kiichiro Tsuchiya ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Intestinal Mucosa ◽  
Intestinal Epithelial Cells ◽  
Core Promoter ◽  
Expression Patterns ◽  
Intestinal Epithelial ◽  
Regulatory Factor ◽  
Human Intestinal Epithelial Cells ◽  
Ifn Γ

ABSTRACT Intestinal epithelial cell-derived interleukin (IL)-7 functions as a pleiotropic and nonredundant cytokine in the human intestinal mucosa; however, the molecular basis of its production has remained totally unknown. We here showed that human intestinal epithelial cells both constitutively and when induced by gamma interferon (IFN-γ) produced IL-7, while several other factors we tested had no effect. Transcriptional regulation via an IFN regulatory factor element (IRF-E) on the 5′ flanking region, which lacks canonical core promoter sequences, was pivotal for both modes of IL-7 expression. IRF-1 and IRF-2, the latter of which is generally known as a transcriptional repressor, were shown to interact with IRF-E and transactivate IL-7 gene expression in an IFN-γ-inducible and constitutive manner, respectively. Indeed, tetracycline-inducible expression experiments revealed that both of these IRF proteins up-regulated IL-7 protein production, and their exclusive roles were further confirmed by small interfering RNA-mediated gene silencing systems. Moreover, these IRFs displayed distinct properties concerning the profile of IL-7 transcripts upon activation and expression patterns within human colonic epithelial tissues. These results suggest that the functional interplay between IRF-1 and IRF-2 serves as an elaborate and cooperative mechanism for timely as well as continuous regulation of IL-7 production that is essential for local immune regulation within human intestinal mucosa.

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