scholarly journals Optimization of Experimental Conditions of Automated Glucuronidation Assays in Human Liver Microsomes Using a Cocktail Approach and Ultra-High Performance Liquid Chromatography–Tandem Mass Spectrometry

2018 ◽  
Vol 47 (2) ◽  
pp. 124-134 ◽  
Author(s):  
Justine Badée ◽  
Nahong Qiu ◽  
Neil Parrott ◽  
Abby C. Collier ◽  
Stephan Schmidt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Liver ◽  
High Performance ◽  
Liver Microsomes ◽  
Tandem Mass ◽  
Experimental Conditions ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Optimization Of Experimental Conditions ◽  
Cocktail Approach

scholarly journals Reactive intermediates in naquotinib metabolism identified by liquid chromatography-tandem mass spectrometry: phase I metabolic profiling

RSC Advances ◽  
2019 ◽  
Vol 9 (18) ◽  
pp. 10211-10225 ◽  
Author(s):  
Mohamed W. Attwa ◽  
Adnan A. Kadi ◽  
Haitham AlRabiah ◽  
Hany W. Darwish
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Metabolic Profiling ◽  
Human Liver ◽  
Liver Microsomes ◽  
Reactive Intermediates ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

LC-MS/MS was used to screen for in vitro metabolites of NQT formed during incubation with human liver microsomes (HLMs) and then evaluated the generation of reactive electrophiles using capturing agents.

Validated assay for the evaluation of multiple glucuronidation activities in human liver microsomes via liquid chromatography-tandem mass spectrometry

RSC Advances ◽  
2014 ◽  
Vol 4 (99) ◽  
pp. 56132-56138 ◽  
Author(s):  
Rong Shi ◽  
Yuanyuan Yang ◽  
Jie Zhong ◽  
Tianming Wang ◽  
Yueming Ma
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Human Liver ◽  
Liver Microsomes ◽  
Tandem Mass ◽  
Activity Determination ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

A faster and more accurate LC-MS/MS method was established for the activity determination of multiple UGT isoforms in HLMs.

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