Involvement of Different Human Glutathione Transferase Isoforms in the Glutathione Conjugation of Reactive Metabolites of Troglitazone

2011 ◽  
Vol 39 (12) ◽  
pp. 2290-2297 ◽  
Author(s):  
Ran Okada ◽  
Kazuya Maeda ◽  
Takahito Nishiyama ◽  
Shinsuke Aoyama ◽  
Zenzaburo Tozuka ◽  
...  
Keyword(s):  
Glutathione Transferase ◽  
Reactive Metabolites ◽  
Glutathione Conjugation

Glutathione conjugation of the fluorophotometric epoxide substrate, 7- glycidoxycoumarin (GOC), by rat liver glutathione transferase isoenzymes

1989 ◽  
Vol 38 (16) ◽  
pp. 2609-2613 ◽  
Author(s):  
Akira Hiratsuka ◽  
Akihiro Yokoi ◽  
Noriyuki Sebata ◽  
Tadashi Watabe ◽  
kimihiko Satoh ◽  
...  
Keyword(s):  
Rat Liver ◽  
Glutathione Transferase ◽  
Liver Glutathione ◽  
Glutathione Conjugation

Enzymatic Detoxification using Lipophilic Hollow-Fiber Membranes: IV. Glutathione Conjugation Reactions

1989 ◽  
Vol 12 (2) ◽  
pp. 121-128 ◽  
Author(s):  
D. Tsikas ◽  
G. Brunner
Keyword(s):  
Hollow Fiber ◽  
Glutathione Transferase ◽  
Hollow Fiber Membrane ◽  
Styrene Oxide ◽  
Acid Methyl Ester ◽  
Covalent Immobilization ◽  
Transferase Activity ◽  
Enzyme Preparations ◽  
Glutathione Conjugation ◽  
Epoxystearic Acid

A hollow-fiber technique was used in the enzymatic glutathione conjugation of lipophilic toxins. Native enzyme was circulated on the external side of a lipophilic hollow-fiber membrane while the toxin-containing media (blood, plasma or aqueous solution) were circulated inside the fiber. Glutathione conjugation reactions were catalyzed by rat liver cytosol, with a specific glutathione transferase activity of 40 nmol/min/mg protein (acceptor: 1,2-epoxy-3-(p-nitrophenoxy)propane). Clearance rates of 1,2-epoxy-3-(p-nitrophenoxy)propane, phenylglycidether, styrene oxide, cis-9, 10-epoxystearic acid, cis-9, 10-epoxystearic acid methyl ester, 5a, 6a-cholesterol oxide, 16-, 17a-pregnenolone oxide, and p-nitrobenzylchloride were 10.44, 13.37, 32.25, 7.60, 7.31, 3.92, 4.20 and 29.24 nmol/mg protein/h/cm2 hollow-fiber surface respectively. This technique makes possible glutathione conjugation reactions with crude enzyme preparations over long periods without loss of activity from covalent immobilization and without loss of cofactor from (auto)oxidation. The lipophilic membrane ensures the absence of hemolysis, immunological hazards and hormone loss, while elimination of the toxin is not impaired.

Glutathione conjugation of styrene 7,8-oxide enantiomers by major glutathione transferase isoenzymes isolated from rat livers

1989 ◽  
Vol 38 (24) ◽  
pp. 4405-4413 ◽  
Author(s):  
Hiratsuka Akira ◽  
Yokoi Akihiro ◽  
Iwata Hiroshi ◽  
Watabe Tadashi ◽  
Satoh Kimihiko ◽  
...  
Keyword(s):  
Glutathione Transferase ◽  
Glutathione Conjugation ◽  
Rat Livers
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