scholarly journals An Intestinal Epithelium-Specific Cytochrome P450 (P450) Reductase-Knockout Mouse Model: Direct Evidence for a Role of Intestinal P450s in First-Pass Clearance of Oral Nifedipine

2008 ◽  
Vol 37 (3) ◽  
pp. 651-657 ◽  
Author(s):  
Qing-Yu Zhang ◽  
Cheng Fang ◽  
Jin Zhang ◽  
Deborah Dunbar ◽  
Laurence Kaminsky ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Mouse Model ◽  
Intestinal Epithelium ◽  
Knockout Mouse ◽  
Direct Evidence ◽  
Knockout Mouse Model ◽  
P450 Reductase ◽  
First Pass

Utility of a Novel Oatp1b2 Knockout Mouse Model for Evaluating the Role of Oatp1b2 in the Hepatic Uptake of Model Compounds

2008 ◽  
Vol 36 (9) ◽  
pp. 1840-1845 ◽  
Author(s):  
Cuiping Chen ◽  
Jeffery L. Stock ◽  
Xingrong Liu ◽  
Jilin Shi ◽  
Jeffrey W. Van Deusen ◽  
...  
Keyword(s):  
Mouse Model ◽  
Knockout Mouse ◽  
Hepatic Uptake ◽  
Model Compounds ◽  
Knockout Mouse Model

Severe protein aggregate myopathy in a knockout mouse model points to an essential role of cofilin2 in sarcomeric actin exchange and muscle maintenance

2014 ◽  
Vol 93 (5-6) ◽  
pp. 252-266 ◽  
Author(s):  
Christine B. Gurniak ◽  
Frédéric Chevessier ◽  
Melanie Jokwitz ◽  
Friederike Jönsson ◽  
Emerald Perlas ◽  
...  
Keyword(s):  
Mouse Model ◽  
Knockout Mouse ◽  
Essential Role ◽  
Protein Aggregate ◽  
Knockout Mouse Model ◽  
Protein Aggregate Myopathy

scholarly journals An inducible intestinal epithelial cell-specific NHE3 knockout mouse model mimicking congenital sodium diarrhea

2020 ◽  
Vol 134 (8) ◽  
pp. 941-953
Author(s):  
Jianxiang Xue ◽  
Linto Thomas ◽  
Maryam Tahmasbi ◽  
Alexandria Valdez ◽  
Jessica A. Dominguez Rieg ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Mouse Model ◽  
Knockout Mouse ◽  
Intestinal Epithelial Cell ◽  
Structural Integrity ◽  
Blood Parameters ◽  
Intestinal Epithelial ◽  
Acid Base ◽  
Knockout Mouse Model

Abstract The sodium–hydrogen exchanger isoform 3 (NHE3, SLC9A3) is abundantly expressed in the gastrointestinal tract and is proposed to play essential roles in Na+ and fluid absorption as well as acid–base homeostasis. Mutations in the SLC9A3 gene can cause congenital sodium diarrhea (CSD). However, understanding the precise role of intestinal NHE3 has been severely hampered due to the lack of a suitable animal model. To navigate this problem and better understand the role of intestinal NHE3, we generated a tamoxifen-inducible intestinal epithelial cell-specific NHE3 knockout mouse model (NHE3IEC-KO). Before tamoxifen administration, the phenotype and blood parameters of NHE3IEC-KO were unremarkable compared with control mice. After tamoxifen administration, NHE3IEC-KO mice have undetectable levels of NHE3 in the intestine. NHE3IEC-KO mice develop watery, alkaline diarrhea in combination with a swollen small intestine, cecum and colon. The persistent diarrhea results in higher fluid intake. After 3 weeks, NHE3IEC-KO mice show a ∼25% mortality rate. The contribution of intestinal NHE3 to acid–base and Na+ homeostasis under normal conditions becomes evident in NHE3IEC-KO mice that have metabolic acidosis, lower blood bicarbonate levels, hyponatremia and hyperkalemia associated with drastically elevated plasma aldosterone levels. These results demonstrate that intestinal NHE3 has a significant contribution to acid–base, Na+ and volume homeostasis, and lack of intestinal NHE3 has consequences on intestinal structural integrity. This mouse model mimics and explains the phenotype of individuals with CSD carrying SLC9A3 mutations.

scholarly journals Mllt10 knockout mouse model reveals critical role of Af10-dependent H3K79 methylation in midfacial development

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Honami Ogoh ◽  
Kazutsune Yamagata ◽  
Tomomi Nakao ◽  
Lisa L. Sandell ◽  
Ayaka Yamamoto ◽  
...  
Keyword(s):  
Mouse Model ◽  
Knockout Mouse ◽  
Critical Role ◽  
Knockout Mouse Model ◽  
H3k79 Methylation
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