Functional Expression and Comparative Characterization of Nine Murine Cytochromes P450 by Fluorescent Inhibition Screening

2008 ◽  
Vol 36 (7) ◽  
pp. 1322-1331 ◽  
Author(s):  
Lesley A. McLaughlin ◽  
Leslie J. Dickmann ◽  
C. Roland Wolf ◽  
Colin J. Henderson
Keyword(s):  
Cytochromes P450 ◽  
Functional Expression ◽  
Comparative Characterization

Functional expression and comparative characterization of four feline P450 cytochromes using fluorescent substrates

Xenobiotica ◽  
2016 ◽  
Vol 47 (11) ◽  
pp. 951-961 ◽  
Author(s):  
Gaku Okamatsu ◽  
Kei Kawakami ◽  
Tetsuya Komatsu ◽  
Takio Kitazawa ◽  
Yasuhiro Uno ◽  
...  
Keyword(s):  
Functional Expression ◽  
Comparative Characterization

A Comprehensive Comparative Characterization of Keratinocytes; The Significant Wound Healing Agents

2016 ◽  
Vol 2 (2) ◽  
pp. 3
Author(s):  
Muhammad Irfan-maqsood ◽  
Hojjat Naderi-Meshkin ◽  
Asieh Heirani-Tabasi ◽  
Monireh Bahrami ◽  
Mahdi Mirahmadi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Comparative Characterization

scholarly journals Functional expression of the eukaryotic proton pump rhodopsin OmR2 in Escherichia coli and its photochemical characterization

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Masuzu Kikuchi ◽  
Keiichi Kojima ◽  
Shin Nakao ◽  
Susumu Yoshizawa ◽  
Shiho Kawanishi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Proton Pump ◽  
Expression System ◽  
Spectroscopic Characterization ◽  
Functional Expression ◽  
Proton Pumping ◽  
E Coli ◽  
Oxyrrhis Marina ◽  
Domains Of Life

AbstractMicrobial rhodopsins are photoswitchable seven-transmembrane proteins that are widely distributed in three domains of life, archaea, bacteria and eukarya. Rhodopsins allow the transport of protons outwardly across the membrane and are indispensable for light-energy conversion in microorganisms. Archaeal and bacterial proton pump rhodopsins have been characterized using an Escherichia coli expression system because that enables the rapid production of large amounts of recombinant proteins, whereas no success has been reported for eukaryotic rhodopsins. Here, we report a phylogenetically distinct eukaryotic rhodopsin from the dinoflagellate Oxyrrhis marina (O. marina rhodopsin-2, OmR2) that can be expressed in E. coli cells. E. coli cells harboring the OmR2 gene showed an outward proton-pumping activity, indicating its functional expression. Spectroscopic characterization of the purified OmR2 protein revealed several features as follows: (1) an absorption maximum at 533 nm with all-trans retinal chromophore, (2) the possession of the deprotonated counterion (pKa = 3.0) of the protonated Schiff base and (3) a rapid photocycle through several distinct photointermediates. Those features are similar to those of known eukaryotic proton pump rhodopsins. Our successful characterization of OmR2 expressed in E. coli cells could build a basis for understanding and utilizing eukaryotic rhodopsins.

Comparative characterization of phosphorylated wood holocelluloses and celluloses for nanocellulose production

Cellulose ◽  
2021 ◽  
Author(s):  
Mengchen Zhao ◽  
Yuko Ono ◽  
Yuichi Noguchi ◽  
Shuji Fujisawa ◽  
Tsuguyuki Saito
Keyword(s):  
Comparative Characterization

scholarly journals Functional expression and characterization of the C. elegans G-protein-coupled FLP-2 Receptor (T19F4.1) in mammalian cells and yeast

2013 ◽  
Vol 3 ◽  
pp. 1-7 ◽  
Author(s):  
Martha J. Larsen ◽  
Elizabeth Ruiz Lancheros ◽  
Tracey Williams ◽  
David E. Lowery ◽  
Timothy G. Geary ◽  
...  
Keyword(s):  
G Protein ◽  
Mammalian Cells ◽  
Functional Expression ◽  
C Elegans ◽  
G Protein Coupled

Synthesis and Comparative Characterization of 9-Boraanthracene, 5-Boranaphthacene, and 6-Borapentacene Stabilized by the H2IMes Carbene

2010 ◽  
Vol 16 (40) ◽  
pp. 12199-12206 ◽  
Author(s):  
Thomas K. Wood ◽  
Warren E. Piers ◽  
Brian A. Keay ◽  
Masood Parvez
Keyword(s):  
Comparative Characterization
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