EFFECT OF CYTOCHROMES P450 CHEMICAL INHIBITORS AND MONOCLONAL ANTIBODIES ON HUMAN LIVER MICROSOMAL ESTERASE ACTIVITY

2006 ◽  
Vol 34 (8) ◽  
pp. 1361-1366 ◽  
Author(s):  
Stacey L. Polsky-Fisher ◽  
Hong Cao ◽  
Ping Lu ◽  
Christopher R. Gibson
Keyword(s):  
Monoclonal Antibodies ◽  
Esterase Activity ◽  
Human Liver ◽  
Cytochromes P450 ◽  
Chemical Inhibitors

scholarly journals Monoester-DiterpeneAconitumAlkaloid Metabolism in Human Liver Microsomes: Predominant Role of CYP3A4 and CYP3A5

2013 ◽  
Vol 2013 ◽  
pp. 1-24 ◽  
Author(s):  
Ling Ye ◽  
Xiao-Shan Yang ◽  
Lin-lin Lu ◽  
Wei-Ying Chen ◽  
Shan Zeng ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Liver ◽  
High Resolution Mass Spectrometry ◽  
Parent Compound ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Diterpene Alkaloids ◽  
Cyp Enzymes ◽  
Chemical Inhibitors

Aconitum, widely used to treat rheumatoid arthritis for thousands of years, is a toxic herb that can frequently cause fatal cardiac poisoning.Aconitumtoxicity could be decreased by properly hydrolyzing diester-diterpene alkaloids into monoester-diterpene alkaloids. Monoester-diterpene alkaloids, including benzoylaconine (BAC), benzoylmesaconine (BMA), and benzoylhypaconine (BHA), are the primary active and toxic constituents of processedAconitum. Cytochrome P450 (CYP) enzymes protect the human body by functioning as the defense line that limits the invasion of toxicants. Our purposes were to identify the CYP metabolites of BAC, BMA, and BHA in human liver microsomes and to distinguish which isozymes are responsible for their metabolism through the use of chemical inhibitors, monoclonal antibodies, and cDNA-expressed CYP enzyme. High-resolution mass spectrometry was used to characterize the metabolites. A total of 7, 8, and 9 metabolites were detected for BAC, BMA, and BHA, respectively. The main metabolic pathways were demethylation, dehydrogenation, demethylation-dehydrogenation, hydroxylation and didemethylation, which produced less toxic metabolites by decomposing the group responsible for the toxicity of the parent compound. Taken together, the results of the chemical inhibitors, monoclonal antibodies, and cDNA-expressed CYP enzymes experiments demonstrated that CYP3A4 and CYP3A5 have essential functions in the metabolism of BAC, BMA, and BHA.

Interaction of isoflavonoids with human liver microsomal cytochromes P450: inhibition of CYP enzyme activities

Xenobiotica ◽  
2016 ◽  
Vol 47 (4) ◽  
pp. 324-331 ◽  
Author(s):  
Michaela Kopečná-Zapletalová ◽  
Kristýna Krasulová ◽  
Pavel Anzenbacher ◽  
Petr Hodek ◽  
Eva Anzenbacherová
Keyword(s):  
Enzyme Activities ◽  
Human Liver ◽  
Cytochromes P450

scholarly journals Multiple-Approaches to the Identification and Quantification of Cytochromes P450 in Human Liver Tissue by Mass Spectrometry

2009 ◽  
Vol 8 (4) ◽  
pp. 1672-1681 ◽  
Author(s):  
Cathrin Seibert ◽  
Brian R. Davidson ◽  
Barry J. Fuller ◽  
Laurence H. Patterson ◽  
William J. Griffiths ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liver Tissue ◽  
Human Liver ◽  
Cytochromes P450 ◽  
Human Liver Tissue ◽  
Identification And Quantification

Inhibitory monoclonal antibodies to human cytochrome P450 1A2: analysis of phenacetin O-deethylation in human liver

1998 ◽  
Vol 8 (5) ◽  
pp. 375-382 ◽  
Author(s):  
Tian J. Yang ◽  
Yang Sai ◽  
Kristopher W. Krausz ◽  
Frank J. Gonzalez ◽  
Harry V. Gelboin
Keyword(s):  
Cytochrome P450 ◽  
Monoclonal Antibodies ◽  
Human Liver ◽  
Cytochrome P450 1A2 ◽  
Human Cytochrome

Immunohistochemical Localization of Cytochromes P450 with Polyclonal and Monoclonal Antibodies

1989 ◽  
Vol 8 (2) ◽  
pp. 61-94 ◽  
Author(s):  
Lucy M. Anderson ◽  
Jerrold M. Ward ◽  
Sang S. Park ◽  
Jerry M. Rice
Keyword(s):  
Monoclonal Antibodies ◽  
Cytochromes P450 ◽  
Immunohistochemical Localization

scholarly journals INTERACTION OF AROMATIC CYTOKININS WITH HUMAN LIVER MICROSOMAL CYTOCHROMES P450

2005 ◽  
Vol 149 (2) ◽  
pp. 349-351 ◽  
Author(s):  
Eva Anzenbacherova ◽  
Jakub Janalik ◽  
Igor Popa ◽  
Miroslav Strnad ◽  
Pavel Anzenbacher
Keyword(s):  
Human Liver ◽  
Cytochromes P450 ◽  
Aromatic Cytokinins
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