Flow of multigrade motor oils in an extensional flow field

1990 ◽  
Vol 34 (8) ◽  
pp. 1373-1386 ◽  
Author(s):  
R. K. Gupta ◽  
R. C. Chan ◽  
A. K. Deysarkar
Keyword(s):  
Flow Field ◽  
Extensional Flow ◽  
Motor Oils

scholarly journals Inducing protein aggregation by extensional flow

2017 ◽  
Vol 114 (18) ◽  
pp. 4673-4678 ◽  
Author(s):  
John Dobson ◽  
Amit Kumar ◽  
Leon F. Willis ◽  
Roman Tuma ◽  
Daniel R. Higazi ◽  
...  
Keyword(s):  
Flow Field ◽  
Protein Aggregation ◽  
Large Scale ◽  
Extensional Flow ◽  
Cysteine Residue ◽  
Hydrodynamic Flow ◽  
Extrinsic Factors ◽  
Aggregation Propensity ◽  
Industrial Manufacture

Relative to other extrinsic factors, the effects of hydrodynamic flow fields on protein stability and conformation remain poorly understood. Flow-induced protein remodeling and/or aggregation is observed both in Nature and during the large-scale industrial manufacture of proteins. Despite its ubiquity, the relationships between the type and magnitude of hydrodynamic flow, a protein’s structure and stability, and the resultant aggregation propensity are unclear. Here, we assess the effects of a defined and quantified flow field dominated by extensional flow on the aggregation of BSA, β2-microglobulin (β2m), granulocyte colony stimulating factor (G-CSF), and three monoclonal antibodies (mAbs). We show that the device induces protein aggregation after exposure to an extensional flow field for 0.36–1.8 ms, at concentrations as low as 0.5 mg mL−1. In addition, we reveal that the extent of aggregation depends on the applied strain rate and the concentration, structural scaffold, and sequence of the protein. Finally we demonstrate the in situ labeling of a buried cysteine residue in BSA during extensional stress. Together, these data indicate that an extensional flow readily unfolds thermodynamically and kinetically stable proteins, exposing previously sequestered sequences whose aggregation propensity determines the probability and extent of aggregation.

Numerical Simulation of Dynamics on Plant Fibers Suspension in Shear-Planar Extensional Flow

2014 ◽  
Vol 1056 ◽  
pp. 66-69 ◽  
Author(s):  
Zan Huang
Keyword(s):  
Differential Equation ◽  
Numerical Simulation ◽  
Flow Field ◽  
Mathematical Simulation ◽  
Extensional Flow ◽  
Simulation Method ◽  
Complex Flow ◽  
Plant Fibers ◽  
Fibers Orientation ◽  
Planar Extensional Flow

A mathematical simulation method is applied to simulate dynamics on plant fibers suspensions in shear-planar extensional flow. Furthermore, the result of differential equation on plant fibers orientation can be obtained in complex flow field.

Effect of interfacial slip on the deformation of a viscoelastic drop in uniaxial extensional flow field

2017 ◽  
Vol 29 (3) ◽  
pp. 032105 ◽  
Author(s):  
Sayan Das ◽  
Shubhadeep Mandal ◽  
S. K. Som ◽  
Suman Chakraborty
Keyword(s):  
Flow Field ◽  
Extensional Flow ◽  
Interfacial Slip ◽  
Uniaxial Extensional Flow
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