Effect of interfacial slip on the deformation of a viscoelastic drop in uniaxial extensional flow field

2017 ◽  
Vol 29 (3) ◽  
pp. 032105 ◽  
Author(s):  
Sayan Das ◽  
Shubhadeep Mandal ◽  
S. K. Som ◽  
Suman Chakraborty
Keyword(s):  
Flow Field ◽  
Extensional Flow ◽  
Interfacial Slip ◽  
Uniaxial Extensional Flow

scholarly journals Rheological behavior of human blood in uniaxial extensional flow

2018 ◽  
Vol 62 (2) ◽  
pp. 447-456 ◽  
Author(s):  
P. C. Sousa ◽  
R. Vaz ◽  
A. Cerejo ◽  
M. S. N. Oliveira ◽  
M. A. Alves ◽  
...  
Keyword(s):  
Human Blood ◽  
Rheological Behavior ◽  
Extensional Flow ◽  
Uniaxial Extensional Flow

scholarly journals Inducing protein aggregation by extensional flow

2017 ◽  
Vol 114 (18) ◽  
pp. 4673-4678 ◽  
Author(s):  
John Dobson ◽  
Amit Kumar ◽  
Leon F. Willis ◽  
Roman Tuma ◽  
Daniel R. Higazi ◽  
...  
Keyword(s):  
Flow Field ◽  
Protein Aggregation ◽  
Large Scale ◽  
Extensional Flow ◽  
Cysteine Residue ◽  
Hydrodynamic Flow ◽  
Extrinsic Factors ◽  
Aggregation Propensity ◽  
Industrial Manufacture

Relative to other extrinsic factors, the effects of hydrodynamic flow fields on protein stability and conformation remain poorly understood. Flow-induced protein remodeling and/or aggregation is observed both in Nature and during the large-scale industrial manufacture of proteins. Despite its ubiquity, the relationships between the type and magnitude of hydrodynamic flow, a protein’s structure and stability, and the resultant aggregation propensity are unclear. Here, we assess the effects of a defined and quantified flow field dominated by extensional flow on the aggregation of BSA, β2-microglobulin (β2m), granulocyte colony stimulating factor (G-CSF), and three monoclonal antibodies (mAbs). We show that the device induces protein aggregation after exposure to an extensional flow field for 0.36–1.8 ms, at concentrations as low as 0.5 mg mL−1. In addition, we reveal that the extent of aggregation depends on the applied strain rate and the concentration, structural scaffold, and sequence of the protein. Finally we demonstrate the in situ labeling of a buried cysteine residue in BSA during extensional stress. Together, these data indicate that an extensional flow readily unfolds thermodynamically and kinetically stable proteins, exposing previously sequestered sequences whose aggregation propensity determines the probability and extent of aggregation.

Dilute Polyacrylamide Solutions under Uniaxial Extensional Flow

Langmuir ◽  
2003 ◽  
Vol 19 (3) ◽  
pp. 559-565 ◽  
Author(s):  
Eric Pelletier ◽  
Christer Viebke ◽  
John Meadows ◽  
Peter A. Williams
Keyword(s):  
Extensional Flow ◽  
Uniaxial Extensional Flow

Structural response of an ordered block copolymer melt to uniaxial extensional flow

Soft Matter ◽  
2014 ◽  
Vol 10 (33) ◽  
pp. 6198-6207 ◽  
Author(s):  
Ruinan Mao ◽  
Erica M. McCready ◽  
Wesley R. Burghardt
Keyword(s):  
Block Copolymer ◽  
Extensional Flow ◽  
Structural Response ◽  
Two Dimensional ◽  
Hexagonally Ordered ◽  
Uniaxial Extensional Flow

Two-dimensional SAXS patterns reveal deformation and re-orientation of microdomains in a hexagonally ordered block copolymer melt subjected to uniaxial extensional flow.

Resistance to uniaxial extensional flow of fibre suspensions

2004 ◽  
Vol 43 (3) ◽  
pp. 223-231 ◽  
Author(s):  
Yong Wee Ooi ◽  
Tamarapu Sridhar
Keyword(s):  
Extensional Flow ◽  
Fibre Suspensions ◽  
Uniaxial Extensional Flow
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