Histotripsy of a collagen tissue-mimicking phantom

2020 ◽  
Vol 148 (4) ◽  
pp. 2801-2801
Author(s):  
Jacob C. Elliott ◽  
Molly Smallcomb ◽  
Julianna Simon ◽  
Meghan E. Vidt ◽  
Sujata Khandare ◽  
...  
Keyword(s):  
Collagen Tissue

Electrospun PLGA and PLGA/gelatin scaffolds for tubularized urethral replacement: Studies in vitro and in vivo

2021 ◽  
pp. 088532822110309
Author(s):  
Jinhua Hu ◽  
Bin Ai ◽  
Shibo Zhu ◽  
Zhen Wang ◽  
Huimin Xia ◽  
...  
Keyword(s):  
Tensile Deformation ◽  
Canine Model ◽  
Urothelial Cells ◽  
Acellular Matrix ◽  
Urethral Strictures ◽  
Acid Copolymer ◽  
Hematoxylin Eosin ◽  
Collagen Tissue

To investigate the biocompatibility of polylactic acid-glycolic acid copolymer (PLGA) and PLGA/gelatin scaffolds and their suitability for tubular urethral replacement in a canine model. PLGA and PLGA/gelatin scaffolds was constructed by electrospinning. Microstructural differences between the scaffolds was examined by Scanning electron microscopy (SEM) followed by mechanical properties testing. Biocompatibility of the material was evaluated using SEM 4, 8, 12 and 72 h after PLGA and PLGA/gelatin scaffolds co-culture with urothelial cells. And confocal analysis was also used to showed the cell adhesive and growth at 12 h. Approximately 2 cm of the anterior urethra of twelve dogs were removed and replaced with a scaffold. After the surgery for 1 month performed urethrography and for 3 month perform hematoxylin–eosin (H&E) and Masson. The results indicated that PLGA and PLGA/gelatin scaffolds had a void microfilament structure, similar to that of normal acellular matrix tissue. And the tensile strength was decreased whereas the tensile deformation and suture retention strength was increased in PLGA/gelatin scaffolds compared to that in PLGA scaffolds Urothelial cells grew well on both scaffolds. Postoperatively, animals recovered well and urinated spontaneously. However, urethrography showed varying degrees of urethral strictures in the reconstructed urethras. H&E and Masson showed that multilayer urothelial cells were formed in both the proximal and distal segments of the reconstructed urethras but without continuity. There was a small amount of smooth muscle and blood vessels under the epithelium, but regenerative urothelial cells at the midpoint of the reconstructed segment did not continue. Lots of lymphocyte infiltration was observed under the epithelium, some collagen tissue was deposited under the neo-urethral epithelium were observed. In conclusion, PLGA and PLGA/gelatin scaffolds are not suitable for tubularized urethral replacement in the canine model.

scholarly journals Can NLR, PLR and LMR be used as prognostic indicators in patients with pulmonary embolism? Author’s reply on commentary

2020 ◽  
Author(s):  
Nuri Kose ◽  
Tarık Yıldırım ◽  
Fatih Akın ◽  
Seda Elçim Yıldırım ◽  
Ibrahim Altun
Keyword(s):  
Inflammatory Diseases ◽  
Emergency Admission ◽  
Prognostic Indicators ◽  
Measuring Time ◽  
Duration Of Symptoms ◽  
Blood Samples ◽  
Inflammatory Parameters ◽  
Inflammatory Drugs ◽  
The Mean ◽  
Collagen Tissue

We appreciate the comments made by Dr Bedel and colleagues. NLR, PLR and LMR are affected by various diseases such as oncological, collagen tissue, inflammatory, or severe renal/liver diseases [1]. Because of this, we have listed some of the above-mentioned disorders in the tables. Hematological diseases, collagen tissue disease, inflammatory diseases, congenital heart disease, or severe renal/liver disease were therefore excluded from the study. However, the presence of malignancy did not affect our results in regression analysis.Platelets swell until 120 minutes in ethylene diamine tetra acetic (EDTA) and until 60 minutes in citrate [2]. Authors suggest that optimal measuring time should not exceed 120 minutes. The blood samples of the patients were taken within 1 hour after their emergency admission. All blood samples in our study were tested within 1 hour of collection [3]. We used EDTA for whole blood anticoagulation. The mean duration of symptoms prior to admission was 5.04 ± 6.9 days. The drugs such as corticosteroids affect inflammatory parameters. Therefore, we excluded inflammatory diseases without emphasizing corticosteroids or other anti-inflammatory drugs.

Diabetes mellitus in anaesthesia

2012 ◽  
Vol 12 (2) ◽  
pp. 60-64 ◽  
Author(s):  
Jadelis Giquel ◽  
Yiliam F Rodriguez-Blanco ◽  
Christina Matadial ◽  
Keith Candiotti
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Immune Deficiency ◽  
Autonomic Neuropathy ◽  
Endocrine System ◽  
Morbidity And Mortality ◽  
Cardiovascular Autonomic Neuropathy ◽  
Operative Morbidity ◽  
Endocrine Diseases ◽  
Collagen Tissue

Anaesthesiologists frequently encounter patients with diseases of the endocrine system, in particular diabetes mellitus. The major risk factors for people with diabetes undergoing surgery are the associated end-organ diseases: cardiovascular autonomic neuropathy, joint collagen tissue, and immune deficiency. Due to the fact that endocrine diseases can be associated with significant peri-operative morbidity and mortality, it is critical that anaesthesiologists understand these disorders and when indicated request the appropriate investigations.

Structural and mechanical characteristics of collagen tissue coated with chitosan in a liquid CO2/water system at different pressures

2019 ◽  
Vol 94 ◽  
pp. 213-221
Author(s):  
Ivan S. Chaschin ◽  
Gennady A. Badun ◽  
Maria G. Chernysheva ◽  
Timofey E. Grigoriev ◽  
Sergey V. Krasheninnikov ◽  
...  
Keyword(s):  
Mechanical Characteristics ◽  
Water System ◽  
Liquid Co2 ◽  
Collagen Tissue

scholarly journals Novel Application of Human Periodontal Ligament Stem Cells and Water-Soluble Chitin for Collagen Tissue Regeneration:In VitroandIn VivoInvestigations

2012 ◽  
Vol 18 (5-6) ◽  
pp. 643-653 ◽  
Author(s):  
Im Hee Jung ◽  
Jung Chul Park ◽  
Jane C. Kim ◽  
Dong Won Jeon ◽  
Seong Ho Choi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Periodontal Ligament ◽  
Water Soluble ◽  
Periodontal Ligament Stem Cells ◽  
Collagen Tissue

scholarly journals Effect of Capacitive Radiofrequency on the Fibrosis of Patients with Cellulite

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Rodrigo Marcel Valentim da Silva ◽  
Priscila Arend Barichello ◽  
Melyssa Lima Medeiros ◽  
Waléria Cristina Miranda de Mendonça ◽  
Jung Siung Camel Dantas ◽  
...  
Keyword(s):  
Blood Circulation ◽  
Collagen Fiber ◽  
Structural Changes ◽  
Active Electrode ◽  
Septal Thickness ◽  
Subcutaneous Adipose ◽  
Consequent Improvement ◽  
Degree Of Severity ◽  
Fiber Thickness ◽  
Collagen Tissue

Background. Cellulite is a type of lipodystrophy that develops primarily from an alteration in blood circulation or of the lymphatic system that causes structural changes in subcutaneous adipose tissue, collagen, and adjacent proteoglycans. The radiofrequency devices used for cutaneous applications have shown different physiological treatment effects, but there is controversy about the suitable parameters for this type of treatment.Objectives. The aim of this study was to evaluate the effects of low-temperature radiofrequency to confirm the thinning of the collagen tissue and interlobular septa and consequent improvement of cellulite.Methods. A sample of eight women was used to collect ultrasonographic data with a 12 MHz probe that measured collagen fiber thickness. The Vip Electromedicina (Argentina) device, frequency of 0.55 MHz and active electrode 3.5 cm in diameter (area = 9.61 cm2), was applied to a 10 cm2region of the gluteal region for 2 minutes per area of active electrode, during 10 biweekly sessions.Results. The Wilcoxon matched paired test was applied using GraphPad InStat 3.01 for Win95-NT software. Pre- and posttreatment mean collagen fiber thickness showed a 24.66% reduction from 1.01 to 0.67 mm. Statistical analysis using the Wilcoxon matched paired test obtained a significant two-tailedPvalue of 0.0391.Conclusion. It was concluded that the use of more comfortable temperatures favored a reduction in fibrous septum thickness and consequent cellulite improvement, evidenced by the lower degree of severity and decrease in interlobular septal thickness.

scholarly journals Impact of dental implant insertion method on the peri-implant bone tissue: Experimental study

2013 ◽  
Vol 70 (9) ◽  
pp. 807-816
Author(s):  
Novak Stamatovic ◽  
Smiljana Matic ◽  
Zoran Tatic ◽  
Aleksandra Petkovic-Curcin ◽  
Danilo Vojvodic ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Collagen Fibers ◽  
Histopathological Analysis ◽  
Moderate Increase ◽  
Tissue Necrosis ◽  
Two Stage ◽  
Bone Implant ◽  
Osteoblastic Activity ◽  
One Stage ◽  
Collagen Tissue

Background/Aim. The function of dental implants depends on their stability in bone tissue over extended period of time, i.e. on osseointegration. The process through which osseointegration is achieved depends on several factors, surgical insertion method being one of them. The aim of this study was to histopathologically compare the impact of the surgical method of implant insertion on the peri-implant bone tissue. Methods. The experiment was performed on 9 dogs. Eight weeks following the extraction of lower premolars implants were inserted using the one-stage method on the right mandibular side and two-stage method on the left side. Three months after implantation the animals were sacrificed. Three distinct regions of bone tissue were histopathologically analyzed, the results were scored and compared. Results. In the specimens of one-stage implants increased amount of collagen fibers was found in 5 specimens where tissue necrosis was also observed. Only moderate osteoblastic activity was found in 3 sections. The analysis of bone-to-implant contact region revealed statistically significantly better results regarding the amount of collagen tissue fibers for the implants inserted in the two-stage method (Wa = 59 < 66,5, ? = 0.05), but necrosis was found in all specimens, and no osteoblastic activity. Histopathological analysis of bone-implant interface of one-stage implants revealed increased amount of collagen fibers in all specimens, moderate osteoblastic activity and neovascularization in 2 specimens. No inflammation was observed. The analysis of two-stage implants revealed a marked increase of collagen fibers in 5 specimens, inflammation and bone necrosis were found in only one specimen. There were no statistically significant differences between the two methods regarding bone-implant interface region. Histopathological analysis of bone tissue adjacent to the one-stage implant revealed moderate increase of collagen tissue in only 1 specimen, moderate increase of osteoblasts and osteocytes in 3 specimens. No necrotic tissue was found. The analyzed specimens of bone adjacent to two-stage implants revealed a moderate increase in the number of osteocytes in 3 and a marked increase in 6 specimens respectively. This difference was statistically significant (Wb = 106.5 > 105, ? = 0.05). No necrosis and osteoblastic activity were observed. Conclusion. Better results were achieved by the two-stage method in bone-to-implant contact region regarding the amount of collagen tissue, while the results were identical regarding the osteoblastic activity and bone tissue necrosis. There was no difference between the methods in the bone-implant interface region. In the bone tissue adjacent to the implant the results were identical regarding the amount of collagen tissue, osteoblastic reaction and bone tissue necrosis, while better results were achieved by the two-stage method regarding the number of osteocytes.

Management of a root fracture in an immature permanent tooth

2006 ◽  
Vol 30 (2) ◽  
pp. 127-130 ◽  
Author(s):  
Kenshi Maki ◽  
Takahiro Nishioka ◽  
Reishi Seo ◽  
Mitsusaka Kimura
Keyword(s):  
Permanent Tooth ◽  
Periodontal Therapy ◽  
Radiographic Examination ◽  
Central Incisor ◽  
Adhesive Resin ◽  
Root Fracture ◽  
Pressure Pain ◽  
Collagen Tissue

A 9-year-old girl with trauma to the maxillary right permanent central incisor is reported. Clinically the tooth appeared extruded with one third of the root visible. The radiographic examination revealed a root fracture. The extruded tooth was repositioned and splinted with a heavy wire and adhesive resin, after which a resorbable collagen tissue was placed to cover the buccal region. Subsequent to the injury, swelling was observed and the patient complained of pressure pain in the periapical region of the tooth with no response to electric pulp testing. It was treated successfully with periodontal therapy and endodontic therapy with Vitapex®

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