scholarly journals Role of freeze-drying in the presence of mannitol on the echogenicity of echogenic liposomes

2017 ◽  
Vol 142 (6) ◽  
pp. 3670-3676 ◽  
Author(s):  
Krishna N. Kumar ◽  
Sanku Mallik ◽  
Kausik Sarkar
Keyword(s):  
Freeze Drying ◽  
Echogenic Liposomes

scholarly journals Glutathione Protects Lactobacillus sanfranciscensis against Freeze-Thawing, Freeze-Drying, and Cold Treatment

2010 ◽  
Vol 76 (9) ◽  
pp. 2989-2996 ◽  
Author(s):  
Juan Zhang ◽  
Guo-Cheng Du ◽  
Yanping Zhang ◽  
Xian-Yan Liao ◽  
Miao Wang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Stress Responses ◽  
Freeze Drying ◽  
Unsaturated Fatty Acids ◽  
Cold Treatment ◽  
Protective Role ◽  
Intracellular Accumulation ◽  
Lactobacillus Sanfranciscensis

ABSTRACT Lactobacillus sanfranciscensis DSM20451 cells containing glutathione (GSH) displayed significantly higher resistance against cold stress induced by freeze-drying, freeze-thawing, and 4°C cold treatment than those without GSH. Cells containing GSH were capable of maintaining their membrane structure intact when exposed to freeze-thawing. In addition, cells containing GSH showed a higher proportion of unsaturated fatty acids in cell membranes upon long-term cold treatment. Subsequent studies revealed that the protective role of GSH against cryodamage of the cell membrane is partly due to preventing peroxidation of membrane fatty acids and protecting Na+,K+-ATPase. Intracellular accumulation of GSH enhanced the survival and the biotechnological performance of L. sanfranciscensis, suggesting that the robustness of starters for sourdough fermentation can be improved by selecting GSH-accumulating strains. Moreover, the results of this study may represent a further example of mechanisms for stress responses in lactic acid bacteria.

Role of Cryoprotectants on the Viability and Functional Properties of Probiotic Lactic Acid Bacteria during Freeze Drying

2009 ◽  
Vol 23 (3) ◽  
pp. 243-265 ◽  
Author(s):  
Kanchi Bhasker Praveen Kumar Reddy ◽  
Sharda Prasad Awasthi ◽  
Arenahalli Ningegowda Madhu ◽  
Siddalingaiya Gurudutt Prapulla
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Functional Properties ◽  
Freeze Drying ◽  
Probiotic Lactic Acid Bacteria

Role of Freeze Drying in Nanotechnology

2007 ◽  
Vol 25 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Guohua Chen ◽  
Wei Wang
Keyword(s):  
Freeze Drying

scholarly journals Role of gellan gum microstructure in freeze drying and rehydration mechanisms

2018 ◽  
Vol 75 ◽  
pp. 51-61 ◽  
Author(s):  
Mattia Cassanelli ◽  
Ian Norton ◽  
Tom Mills
Keyword(s):  
Freeze Drying ◽  
Gellan Gum

scholarly journals Pectin as Rheology Modifier of a Gelatin-Based Biomaterial Ink

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 3109
Author(s):  
Anna Lapomarda ◽  
Elena Pulidori ◽  
Giorgia Cerqueni ◽  
Irene Chiesa ◽  
Matteo De Blasi ◽  
...  
Keyword(s):  
Rheological Properties ◽  
Freeze Drying ◽  
Crosslinking Agent ◽  
Three Dimensional ◽  
Compressive Modulus ◽  
Crosslinking Reaction ◽  
Intrinsic Properties ◽  
Pectin Gels ◽  
Rheology Modifier

Gelatin is a natural biopolymer extensively used for tissue engineering applications due to its similarities to the native extracellular matrix. However, the rheological properties of gelatin formulations are not ideal for extrusion-based bioprinting. In this work, we present an approach to improve gelatin bioprinting performances by using pectin as a rheology modifier of gelatin and (3-glycidyloxypropyl)trimethoxysilane (GPTMS) as a gelatin–pectin crosslinking agent. The preparation of gelatin–pectin formulations is initially optimized to obtain homogenous gelatin–pectin gels. Since the use of GPTMS requires a drying step to induce the completion of the crosslinking reaction, microporous gelatin–pectin–GPTMS sponges are produced through freeze-drying, and the intrinsic properties of gelatin–pectin–GPTMS networks (e.g., porosity, pore size, degree of swelling, compressive modulus, and cell adhesion) are investigated. Subsequently, rheological investigations together with bioprinting assessments demonstrate the key role of pectin in increasing the viscosity and the yield stress of low viscous gelatin solutions. Water stable, three-dimensional, and self-supporting gelatin–pectin–GPTMS scaffolds with interconnected micro- and macroporosity are successfully obtained by combining extrusion-based bioprinting and freeze-drying. The proposed biofabrication approach does not require any additional temperature controller to further modulate the rheological properties of gelatin solutions and it could furthermore be extended to improve the bioprintability of other biopolymers.

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