SU-D-137-06: Effect of Dose-Per-Pulse and Pulse Repetition Frequency in X-Ray Beam Irradiation of In-Vitro Cell Cultures

2013 ◽  
Vol 40 (6Part3) ◽  
pp. 103-103
Author(s):  
G Lasio ◽  
M Guerrero ◽  
W Goetz ◽  
F Lima ◽  
J Baulch
Keyword(s):  
Cell Cultures ◽  
Pulse Repetition Frequency ◽  
Repetition Frequency ◽  
Pulse Repetition ◽  
Beam Irradiation ◽  
X Ray ◽  
In Vitro Cell Cultures

scholarly journals Effects of Non-thermal Ultrasound on a Fibroblast Monolayer Culture: Influence of Pulse Number and Pulse Repetition Frequency

Sensors ◽  
2021 ◽  
Vol 21 (15) ◽  
pp. 5040
Author(s):  
Silvia Ronda Peñacoba ◽  
Mar Fernández Gutiérrez ◽  
Julio San Román del Barrio ◽  
Francisco Montero de Espinosa
Keyword(s):  
Short Pulse ◽  
Pulse Repetition Frequency ◽  
Pulse Number ◽  
Repetition Frequency ◽  
Pulse Repetition ◽  
Treatment Protocols ◽  
Culture Plate ◽  
New Treatment ◽  
Long Pulse

Despite the use of therapeutic ultrasound in the treatment of soft tissue pathologies, there remains some controversy regarding its efficacy. In order to develop new treatment protocols, it is a common practice to carry out in vitro studies in cell cultures before conducting animal tests. The lack of reproducibility of the experimental results observed in the literature concerning in vitro experiments motivated us to establish a methodology for characterizing the acoustic field in culture plate wells. In this work, such acoustic fields are fully characterized in a real experimental configuration, with the transducer being placed in contact with the surface of a standard 12-well culture plate. To study the non-thermal effects of ultrasound on fibroblasts, two different treatment protocols are proposed: long pulse (200 cycles) signals, which give rise to a standing wave in the well with the presence of cavitation (ISPTP max = 19.25 W/cm2), and a short pulse (five cycles) of high acoustic pressure, which produces a number of echoes in the cavity (ISPTP = 33.1 W/cm2, with Pmax = 1.01 MPa). The influence of the acoustic intensity, the number of pulses, and the pulse repetition frequency was studied. We further analyzed the correlation of these acoustic parameters with cell viability, population, occupied surface, and cell morphology. Lytic effects when cavitation was present, as well as mechanotransduction reactions, were observed.

Influence of pulse repetition frequency and high pass filter on color Doppler maps of converging flow in vitro

1996 ◽  
Vol 12 (4) ◽  
pp. 257-261 ◽  
Author(s):  
Martin Giesler ◽  
Veit Göller ◽  
Alexander Pfob ◽  
Dionyz Bajtay ◽  
Matthias Kochs ◽  
...  
Keyword(s):  
Pulse Repetition Frequency ◽  
Color Doppler ◽  
Repetition Frequency ◽  
Pulse Repetition ◽  
Pass Filter ◽  
High Pass Filter ◽  
Converging Flow ◽  
High Pass

scholarly journals Influence of changing pulse repetition frequency on chemical and biological effects induced by low-intensity ultrasound in vitro

2009 ◽  
Vol 16 (3) ◽  
pp. 392-397 ◽  
Author(s):  
Mikhail A. Buldakov ◽  
Mariame A. Hassan ◽  
Qing-Li Zhao ◽  
Loreto B. Feril ◽  
Nobuki Kudo ◽  
...  
Keyword(s):  
Biological Effects ◽  
Pulse Repetition Frequency ◽  
Repetition Frequency ◽  
Pulse Repetition ◽  
Low Intensity ◽  
Low Intensity Ultrasound

scholarly journals Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 490
Author(s):  
Martin Sák ◽  
Ivana Dokupilová ◽  
Šarlota Kaňuková ◽  
Michaela Mrkvová ◽  
Daniel Mihálik ◽  
...  
Keyword(s):  
Cell Wall ◽  
Vitis Vinifera ◽  
Cell Cultures ◽  
Trichoderma Viride ◽  
Vitis Vinifera L ◽  
Treated Plant ◽  
Abiotic Elicitors ◽  
In Vitro Cell Cultures ◽  
Red Grape

The in vitro cell cultures derived from the grapevine (Vitis vinifera L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from Trichoderma viride, the cell wall homogenate from Fusarium oxysporum and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds trans-resveratrol, trans-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from F. oxysporum. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from T. viride. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of trans-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates.

Aqueous Benzene Oxidation in Low-Temperature Plasma of Pulsed Corona Discharge

2016 ◽  
Vol 19 (2) ◽  
Author(s):  
Iakov Kornev ◽  
Sergei Preis
Keyword(s):  
Low Temperature ◽  
Corona Discharge ◽  
Gas Phase ◽  
Pulse Repetition Frequency ◽  
Repetition Frequency ◽  
Pulse Repetition ◽  
Benzene Oxidation ◽  
Low Temperature Plasma ◽  
Temperature Plasma ◽  
Pulsed Corona

AbstractWastewaters polluted with non-biodegradable volatile organic compounds (VOCs), such as aromatic substances, present a growing problem meeting no adequately affordable technological response. Low-temperature plasma generated in the gas-phase pulsed corona discharge (PCD) presents competitive advanced oxidation technology in abatement of various classes of pollutants, although the process parameters, the pulse repetition frequency and the liquid spray rate, require optimization. The experimental research into aqueous benzene oxidation with PCD was undertaken to establish the impact of the parameters to the energy efficiency. The oxidation reaction was found under the experimental conditions to mostly proceed in the gas phase showing little influence of the pulse repetition frequency and the gas-liquid contact surface. Oxidation of benzene and, presumably, other volatile pollutants in the volume of PCD reactor compartment presents an effective strategy of aqueous VOCs abatement.

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