SU-FF-J-104: In Vitro Model to Study the Biological Effect of Dose Gradients On a Cell Culture System

2005 ◽  
Vol 32 (6Part6) ◽  
pp. 1944-1944 ◽  
Author(s):  
R Bromley ◽  
L Oliver ◽  
R Harvie ◽  
R Davey
Keyword(s):  
Cell Culture ◽  
Biological Effect ◽  
Culture System ◽  
In Vitro Model ◽  
Cell Culture System ◽  
A Cell ◽  
Vitro Model

scholarly journals Isospora suis in an Epithelial Cell Culture System – An In Vitro Model for Sexual Development in Coccidia

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e69797 ◽  
Author(s):  
Hanna Lucia Worliczek ◽  
Bärbel Ruttkowski ◽  
Lukas Schwarz ◽  
Kirsti Witter ◽  
Waltraud Tschulenk ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Sexual Development ◽  
Culture System ◽  
In Vitro Model ◽  
Cell Culture System ◽  
Epithelial Cell Culture ◽  
Isospora Suis ◽  
Vitro Model

scholarly journals Characterization of a Cell Culture System of Persistent Hepatitis E Virus Infection in the Human HepaRG Hepatic Cell Line

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 406
Author(s):  
Marie Pellerin ◽  
Edouard Hirchaud ◽  
Yannick Blanchard ◽  
Nicole Pavio ◽  
Virginie Doceul
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Inhibitory Effect ◽  
Cell Culture System ◽  
Efficient Replication ◽  
A Cell ◽  
Vitro Model

Hepatitis E virus (HEV) is considered as an emerging global health problem. In most cases, hepatitis E is a self-limiting disease and the virus is cleared spontaneously without the need of antiviral therapy. However, immunocompromised individuals can develop chronic infection and liver fibrosis that can progress rapidly to cirrhosis and liver failure. The lack of efficient and relevant cell culture system and animal models has limited our understanding of the biology of HEV and the development of effective drugs for chronic cases. In the present study, we developed a model of persistent HEV infection in human hepatocytes in which HEV replicates efficiently. This HEV cell culture system is based on differentiated HepaRG cells infected with an isolate of HEV-3 derived from a patient suffering from acute hepatitis E. Efficient replication was maintained for several weeks to several months as well as after seven successive passages on HepaRG naïve cells. Moreover, after six passages onto HepaRG, we found that the virus was still infectious after oral inoculation into pigs. We also showed that ribavirin had an inhibitory effect on HEV replication in HepaRG. In conclusion, this system represents a relevant and efficient in vitro model of HEV replication that could be useful to study HEV biology and identify effective antiviral drugs against chronic HEV infection.

A Perfused Two-Chamber System for Anticancer Drug Screening

2010 ◽  
Author(s):  
Liang Ma ◽  
Jeremy Barker ◽  
Changchun Zhou ◽  
Biaoyang Lin ◽  
Wei Li
Keyword(s):  
Cell Culture ◽  
Cancer Cells ◽  
Anticancer Drug ◽  
Drug Screening ◽  
Culture System ◽  
Liver Cells ◽  
Cell Culture System ◽  
Chamber System ◽  
A Cell

A cell culture microfluidic device has been developed to test the cytotoxicity of anticancer drugs while reproducing multi-organ interactions in vitro. Cells were cultured in separate chambers representing the liver and tumor. The two chambers were connected through a channel to mimick the blood flow. Glioblastoma (GBM) cancer cells (M059K) and hepatoma cells (HepG2) were cultured in the tumor and the liver chambers, respectively. The cytotoxic effect of cancer treatment drug Temolozomide (TMZ) was tested using this two chamber system. The experimental results showed that with the liver cells, the cancer cells showed much higher viability than those without the liver cells. This indicates that the liver metabolism has strong effect on the toxicity of the anticancer drug. The results demonstrated that the perfused two chamber cell culture system has the potential to be used as a platform for drug screening in a more physiologically realistic environment.

A New In Vitro Model for Predicting the Toxicity of Cosmetic Products

1992 ◽  
Vol 20 (1) ◽  
pp. 138-143
Author(s):  
Maria Carrara ◽  
Lorenzo Cima ◽  
Roberto Cerini ◽  
Maurizio Dalle Carbonare
Keyword(s):  
Cell Culture ◽  
Cultured Cells ◽  
Neutral Red ◽  
Total Protein Content ◽  
Cosmetic Products ◽  
Cell Culture Insert ◽  
A Cell ◽  
Vitro Model ◽  
Cosmetic Emulsions

A method has been developed whereby cosmetic products which are not soluble in water or in alcohol can be brought into contact with cell cultures by being placed in a cell culture insert, which is then placed in the cell culture well. Preliminary experiments were carried out with L929 cells, and cytotoxicity was evaluated by measuring neutral red uptake and the total protein content of treated cultured cells. Encouraging results were obtained in comparisons of three cosmetic emulsions and of one emulsion containing a range of concentrations of two preservatives, Kathon CG and Bronopol.

In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

2020 ◽  
Vol 45 (5) ◽  
pp. 631-637
Author(s):  
Cansu Ozel-Tasci ◽  
Gozde Pilatin ◽  
Ozgur Edeer ◽  
Sukru Gulec
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Metabolic Diseases ◽  
Enzymatic Digestion ◽  
Cell Culture System ◽  
Culture Studies ◽  
Experimental Approaches ◽  
In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

A Cell Culture System for Study of the Development of Toxoplasma gondii Bradyzoites

1995 ◽  
Vol 42 (2) ◽  
pp. 150-157 ◽  
Author(s):  
LOUIS M. WEISS ◽  
DENISE LAPLACE ◽  
PETER M. TAKVORIAN ◽  
HERBERT B. TANOWITZ ◽  
ANN CALI ◽  
...  
Keyword(s):  
Cell Culture ◽  
Toxoplasma Gondii ◽  
Culture System ◽  
Cell Culture System ◽  
A Cell

scholarly journals ISOLATION OF VIRUS CAUSING HEMORRHAGIC FEVER WITH RENAL SYNDROME (HFRS) THROUGH A CELL CULTURE SYSTEM

1983 ◽  
Vol 36 (1) ◽  
pp. 17-25 ◽  
Author(s):  
TAKASHI KITAMURA ◽  
TOSHIHIKO KOMATSU ◽  
KAZUYOSHI SUGIYAMA ◽  
CHIHARU MORITA ◽  
KIYOSHI IMAIZUMI ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Hemorrhagic Fever ◽  
Cell Culture System ◽  
A Cell
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